Diferentes suplementos no cultivo in vitro de embriões de pinhão-manso

Nunes, Claudinéia Ferreira; Pasqual, Moacir; Santos, Dalilhia Nazaré dos; Custódio, Telde Natel; Araujo, Aparecida Gomes de

doi:10.1590/s0100-204x2008000100002

Cited by 33 publications

(36 citation statements)

References 13 publications

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“…Activated charcoal is one of the compounds used to induce root formation in vitro, and it aids in the adsorption of excess toxic substances in the culture medium, such as phenols and growth regulators. It also reduces in the incidence of light in the active growth zone of the root system, which stimulates the formation and lengthening of roots (BONGA, 1985) as they search for light (NUNES et al, 2008). This principle explains the linearly positive action of the expansion of the roots of barueiros.…”

Section: Resultsmentioning

confidence: 99%

In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

Silva

Asmar

Oliveira

et al. 2016

SCA

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The barueiro (Dipteryx alata Vog.) is a native fruit species of the Cerrado ecoregion that has multiple uses. It is a wild species, and its cultivation is difficult. Furthermore, it is threatened with extinction. Plant tissue culture is a major tool for the conservation of germplasm, as well as a means of propagating highquality seedlings on a large scale. However, this technique has not been used with barueiro, although it might provide valuable contributions to the process of barueiro domestication. The most popular method of cultivation is the use of the Murashige and Skoog medium (MS), which is considered one of the most nutritionally complete media. Woody plant medium (WPM) is indicated for the propagation of woody species, but there are no reports of its use for barueiro cultivation. Woody plants tend to have problems with rust in vitro during the establishment phase. Activated charcoal acts as an adjuvant for the adsorption of phenolic compounds, mitigating its effects in the medium. Thus, the objective of this study was to test four activated charcoal doses (0, 2, 4 and 6 g L -1 ) and three culture media: MS, WPM, and AA (over water agar) in the in vitro establishment of barueiro. The experimental design was a completely randomised (DIC), 4 × 3 factorial design with three replications. At 60 days after inoculation, the explants were evaluated for dry matter, fresh weight, stem diameter, shoot length, number of leaves, longest root length, germination rate, and chlorophyll contents. The MS medium supplemented with 3,0 g L -1 activated charcoal appeared to be the best for in vitro establishment of barueiro. Key words: Native fruit. Activated charcoal. Culture medium. ResumoO barueiro (Dipteryx alata Vog.) é uma espécie frutífera nativa do Cerrado que possui múltiplas possibilidades de utilização. Por estar em estado praticamente selvagem, seu cultivo é dificultado. Além disso, encontra-se ameaçada de extinção. A cultura de tecidos vegetais constitui uma importante ferramenta para a conservação de germoplasma, além de proporcionar a rápida propagação de mudas de qualidade em larga escala. Porém, esta técnica não vem sendo utilizada em barueiro, mas pode ser aplicada de forma valiosa contribuindo para seu processo de domesticação. O meio de cultivo mais utilizado é o MS, considerado um dos mais completos nutricionalmente. O meio WPM é indicado para a propagação de espécies lenhosas, porém não há relatos da sua utilização para o cultivo do barueiro.

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Section: Resultsmentioning

confidence: 99%

In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

Silva

Asmar

Oliveira

et al. 2016

SCA

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“…A água de coco fornece açúcares e outros metabólitos para embriões cultivados. Vários trabalhos citam sua utilização no cultivo in vitro de espécies como orquídeas (Araujo et al, 2006) e pinhão-manso (Nunes et al, 2008).…”

Section: Introductionunclassified

Otimização de um protocolo para micropropagação da oliveira Ascolano 315

Villa

Pasqual²,

Freitas³

2010

Rev. Ceres

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Optimization of a protocol for the micropropagation of olive tree cv. Ascolano 315Micropropagation can be a viable technique for the multiplication of olive trees. The objective of this work was to induce multiplication in explants of olive tree. Nodal segments with 2 cm length, without leaves, derived from in vitro plantlets of cultivar Ascolano 315 were excised and inoculated in test tubes. The tubes contained 15 mL of OM (Olive medium) culture medium supplemented with 2 g L -1 of activated charcoal, 4 concentrations of 6-benzilaminopurin (BAP) and 4 concentrations of coconut water and solidified with 5.5 g L -1 of agar. The pH was adjusted to 5.8 before medium sterilization at 121ºC and 1 atm for 20 min. The experimental design was complete randomized in a 4 x 4 factorial scheme. The explants were kept in a growth room 25±1ºC, light intensity of 32 ì mol m -2 s -1 and photoperiod of 16 hours, for 70 days. The culture medium OM added of 1.0 mg L -1 of BAP and 100 mL L -1 of coconut water provided the greatest length and biomass of the aerial part. The largest number of roots was obtained with 0.5 mg L -1 of BAP associated with 25 mL L -1 of coconut water.

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“…É o caso de trabalhos de suplementação dos meios de cultura com fontes exógenas de carboidratos, que favorecem a germinação in vitro de Jatropha curcas em frutos imaturos (NUNES et al, 2008). Essa suplementação é importante tanto para maximizar a taxa de germinação dessa espécie quanto para obter plântulas com elevado potencial fisiológico para serem utilizadas como fonte de explante, em estudos sobre embriogênese somática ou organogênese.…”

Section: O Objetivo Do Presente Trabalho Foi Avaliar In Vitro a Relaçunclassified