Dietary sodium butyrate protects lipopolysaccharide-induced inflammatory response in lambs through inhibiting TLR4/NF-κB signalling pathway

Abstract

This study evaluated the effects of dietary coated sodium butyrate (CSB) on lipopolysaccharide (LPS)-induced inflammatory response in weaned lambs. A basal diet was supplemented with CSB at 0, 2 or 3 g/kg feed for 28 days and/or intraperitoneally injected with 100 lg/kg BW Escherichia coli LPS (0 g/kg CSB, 0 g/kg CSB and LPS, 2 g/kg CSB and LPS, 3 g/kg CSB and LPS). The results showed that CSB supplementation conferred significant protective effects against LPS-induced inflammatory response by reducing in the levels of D-lactate (DLA), diamine oxidase (DAO), inflammatory cytokines (TNF-a, IL-1b and IL-8) in the serum and upregulated expression of tight junction proteins (claudin-3, ZO-1 and occludin) in the ileum. Meanwhile, CSB pre-treatment significantly suppressed LPS-induced TLR4/NF-jB signalling pathway mRNA expression (MyD88, TLR4, NF-jB, IL-6 and IL-1b) and down-regulated TLR4/NF-jB signalling pathway protein expression (MyD88, TLR4, NF-jB p65 and p-NF-jB p65). Furthermore, dietary CSB supplementation improved intestinal morphology. Lastly, bacterial 16S rRNA gene sequencing indicated that CSB could alter intestinal microbiota composition at genus levels. Taken together, our results indicate CSB could maintain intestinal health, improve intestinal morphology, modulate the microbial community and decrease LPS-induced inflammatory response in lambs.

Combined Omics Analysis Further Unveils the Specific Role of Butyrate in Promoting Growth in Early-Weaning Animals

“…Sodium butyrate, a fatty acid salt, induces the functional maturation of intestinal epithelial cells by promoting their proliferation and inhibiting their apoptosis, thereby improving digestion and fostering intestinal mucosal development [ 27 , 28 , 29 ]. It has been reported that the supplementation of the diets of weaning piglets, calves, and lambs with an appropriate amount of butyrate can enhance their disease resistance, improve the intestinal microbiome, and increase growth performance [ 30 , 31 , 32 ]. In this study, we found that, in the weaned rabbits, dietary butyrate supplementation significantly increased the average daily feed intake and the average daily weight gain while reducing the rate of diarrhea.…”

Effect of tea polyphenols on intestinal barrier and immune function in weaned lambs

“…The depth of crypt determines the speed of epithelial cell formation by mitosis of intestinal villi, and reflects the colonization rate and maturity of crypt cells. Therefore, the value of villus height/ crypt depth is an important index to measure intestinal development and function (17,18). In addition, some studies have shown that there is a positive correlation between villi length, nutrient absorption and digestibility.…”