Dietary resveratrol administration increases MnSOD expression and activity in mouse brain

Robb, Ellen L.; Winkelmolen, Lieke; Visanji, Naomi P.; Brotchie, Jonathan M.; Stuart, Jeffrey A.

doi:10.1016/j.bbrc.2008.05.028

Cited by 102 publications

(66 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On this issue, it is well known that resveratrol potentiates mitochondrial activity. 28 In agreement, we found in ex vivo HD STA that 6 hours of incubation with resveratrol induced significant overexpression of MnSOD, a main antioxidant defense mechanism of mitochondria, but did not alter Cu-Zn SOD, which is mainly localized in the cytoplasmic fraction ( Figure 3E). In contrast, incubation with exogenous BH 4 did not significantly influence MnSOD expression (data not shown).…”

supporting

confidence: 88%

Resveratrol Improves Vascular Function in Patients With Hypertension and Dyslipidemia by Modulating NO Metabolism

et al. 2013

View full text Add to dashboard Cite

Abstract-Epidemiological studies have demonstrated that the Mediterranean diet, which is rich in resveratrol, is associated with a significantly reduced risk of cardiovascular disease. However, the molecular mechanisms that underlie the beneficial effects of resveratrol on cardiovascular function remain incompletely understood. Therefore, we set out to identify the molecular target(s) mediating the protective action of resveratrol on vascular function. To this end, we performed vascular reactivity studies to evaluate the effects of resveratrol on superior thyroid artery obtained from 59 patients with hypertension and dyslipidemia. We found that resveratrol evoked vasorelaxation and reduced endothelial dysfunction through the modulation of NO metabolism via (1) an 5′ adenosine monophosphate-activated protein kinase-mediated increase in endothelial NO synthase activity; (2) a rise in tetrahydrobiopterin levels, which also increases endothelial NO synthase activity; and (3) attenuation of vascular oxidative stress, brought about by overexpression of manganese superoxide dismutase via an nuclear factor erythroid-derived 2-like 2-dependent mechanism. The effects of resveratrol on acetylcholine vasorelaxation were also tested in vessels from patients with nonhypertensive nondyslipidemia undergoing thyroid surgery. In this setting, resveratrol failed to exert any effect. Thus, our finding that resveratrol reduces endothelial dysfunction, an early pathophysiological feature and independent predictor of poor prognosis in most forms of cardiovascular disease, supports the concept that the risk of vascular events could be further reduced by adherence to a set of dietary and behavioral guidelines. MethodsFor detailed methodology, please see the online-only Data Supplement. In brief, we collected superior thyroid artery (STA) from patients with HD (Table). Each vessel was divided into rings for use in different experimental series. Some rings were treated with resveratrol at 37°C, and then proteins were rapidly extracted for molecular studies to evaluate total and phosphorylated endothelial NO synthase (eNOS) protein levels, eNOS dimerization, GTP cyclohydrolase 1, copper-zinc superoxide dismutase (Cu-ZnSOD) and MnSOD expression, and NRF2 translocation; other rings were kept in 4°C Krebs solution for vascular reactivity studies; some were used for highperformance liquid chromatography (HPLC) dosage of BH 4 ; and others were rapidly used for measurement of O 2 − . Studies were also performed on vessels removed from patients with nonhypertensive, nondyslipidemia (nHD) undergoing thyroid surgery. Results Resveratrol Exerts a Vasorelaxant Effect by Modulating the Phosphorylation Status of eNOS and NO ReleaseEvaluation of the vascular reactivity of ex vivo STA from patients with HD revealed that endothelially mediated (acetylcholine-evoked) vasorelaxation was significantly blunted compared with relaxation induced by nitroglycerin ( Figure 1A). However, when HD STA rings were preconstricted with phenylephrine, resveratrol was ca...

show abstract

supporting

confidence: 88%

Resveratrol Improves Vascular Function in Patients With Hypertension and Dyslipidemia by Modulating NO Metabolism

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In addition to its antioxidant properties, it has been shown that RES progressively increases the protein levels and the activity of an antioxidant enzyme, MnSOD, in MRC-5 cells 41) and induces SIRT1 protein expression which promotes DNA repair and the maintenance of genome stability 42) . In our study model, RES treatment significantly attenuated SSB and 8-OHdG levels in DNA, consequently DNA damage, following dentin bonding agent treatments.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

View full text Add to dashboard Cite

Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

show abstract

“…Increasing levels of ROS have also been shown to restrict the lifespan of hematopoietic stem cells 41) . Recently, Robb et al demonstrated that RV can markedly induce mitochondrial MnSOD expression and activity in MRC-5 cells, as well as in mouse brain tissues 42) . Moreover, RV has been shown to protect against ROS-induced cell death by activating AMP-activated kinase in cardiac muscle cells 43) .…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Protects Human Endothelium from H2O2-Induced Oxidative Stress and Senescence via SirT1 Activation

Kao

Chen

Chang

et al. 2010

JAT

218

143

View full text Add to dashboard Cite

Dietary resveratrol administration increases MnSOD expression and activity in mouse brain

Cited by 102 publications

References 34 publications

Resveratrol Improves Vascular Function in Patients With Hypertension and Dyslipidemia by Modulating NO Metabolism

Resveratrol Improves Vascular Function in Patients With Hypertension and Dyslipidemia by Modulating NO Metabolism

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Resveratrol Protects Human Endothelium from H2O2-Induced Oxidative Stress and Senescence via SirT1 Activation

Contact Info

Product

Resources

About