Resveratrol Protects Human Endothelium from H2O2-Induced Oxidative Stress and Senescence via SirT1 Activation

Kao, Chung Lan; Chen, Liang Kung; Chang, Yuh Lih; Yung, Ming Chih; Hsu, Chuan Chih; Chen, Yu‐Chih; Lo, Wen-Liang; Chen, Shih‐Jen; Ku, Hung Hai; Hwang, Shin Jang

doi:10.5551/jat.4333

Cited by 218 publications

(148 citation statements)

References 44 publications

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“…Pretreatment with astaxanthin decreased ROS production in response to both acute and long term stimulation with H2O2. Resveratrol, epigallocatechin gallate, quercetin, and a combination of simvastatin and nifedipine also have been shown to reduce ROS production in HUVEC stimulated with H2O2 (Choi et al, 2005;Chen et al, 2010;Kao et al, 2010). Therefore, stimulation with H2O2 suppressed antioxidant function, increased ROS accumulation, and induced oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Chew¹,

Mathison²,

Kimble³

et al. 2013

AJAFST

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Oxidative stress and inflammation are strongly linked to the development of cardiovascular disease (CVD). As a potent antioxidant, astaxanthin may downregulate inflammation-associated factors involved in endothelial dysfunction and CVD progression. We studied the possible protective effects of astaxanthin against endothelial dysfunction in human umbilical vein endothelial cells (HUVEC) induced with hydrogen peroxide (H2O2). Cells were pre-incubated with 0, 0.01, 0.1, or 1.0 μmol/L astaxanthin for 48 h, then oxidative stress induced with 100 μmol/L H2O2 overnight. Uptake kinetics of astaxanthin showed a time-dependent uptake of astaxanthin (1.0 μmol/L) by HUVEC over the 48 h incubation period. Oxidative stress induction with H2O2 in HUVEC decreased intracellular antioxidant activity and increased the production of inflammatory biomarkers and reactive oxygen species (ROS). In contrast, pre-incubation of HUVEC with astaxanthin prior to H2O2 stress increased (P < 0.05) superoxide dismutase activity and decreased ROS, prostaglandin E2, leukotriene B4, NO, IL-8, and IFN- production. Pre-treatment with astaxanthin also downregulated the transcriptional activation of NF-κB and activator protein-1, thereby inhibiting downstream production of inflammatory mediators and cytokines. Therefore, astaxanthin protects against factors initiated by H2O2 addition, likely by scavenging ROS required for transcriptional activation and inhibiting the production of inflammatory biomarkers involved in endothelial dysfunction and CVD.Abbreviations: AP-1: activator protein-1; CVD: cardiovascular disease; CS: culture supernatant; EGM-2: endothelial growth medium-2; GPx: glutathione peroxidase; H2O2: hydrogen peroxide; HUVEC: human umbilical vein endothelial cell; LTB4: leukotriene B4; PGE2: prostaglandin E2; ROS: reactive oxygen species; SOD: superoxide dismutase; THF: tetrahydrofuran.

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Section: Discussionmentioning

confidence: 99%

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Chew¹,

Mathison²,

Kimble³

et al. 2013

AJAFST

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“…Kao et al demonstrated that RSV reduces reactive oxygen species (ROS) production in human endothelium exposed to H2O2 8) . We also found that RSV inhibited ROS expression of Ets-2 (Fig.…”

Section: Ets-1 Mediated the Induction Of The Mir-126 Expression By Rsvmentioning

confidence: 99%

“…Resveratrol (RSV), an edible polyphenolic phytoalexin present in grapes, peanuts and other plant species, provides protection against numerous ageassociated diseases, including cancer, Alzheimer's disease and cardiovascular disease [5][6][7] . Both in vivo and in vitro treatment with RSV results in reduced markers of oxidative stress 8) . It has been reported that RSV plays an important role in the antioxidation of human umbilical vascular endothelial cells (HUVEC) 9) .…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Inhibits Hydrogen Peroxide-Induced Apoptosis in Endothelial Cells via the Activation of PI3K/Akt by miR-126

Sui

Xie

et al. 2014

JAT

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Aim: Resveratrol (RSV) is an edible polyphenolic phytoalexin present in different plant species that plays an important role in improving endothelial dysfunction. However, the molecular mechanisms underlying these effects are unknown. In the present study, the mechanism underlying the protection of CRL-1730 cells by RSV against oxidative stress was examined. Methods: We first assessed the effects of RSV on the cell viability and apoptosis of CRL-1730 cells exposed to hydrogen peroxide (H2O2). Real-time PCR was used to determine the microRNA-126 (miR-126) expression in cells treated with RSV and/or H2O2. We also evaluated the PI3K/Akt signaling pathway in CRL-1730 cells following upregulation of the miR-126 expression. Finally, we determined the effects of miR-126 on RSV against oxidative injury using an miR-126 inhibitor. Results: Treatment with RSV resulted in a significant increase in survival and a decrease in the apoptosis of CRL-1730 cells exposed to H2O2. We also found that H2O2 significantly suppressed the expression of miR-126, which was reversed by RSV in a dose-dependent manner. The overexpression of miR-126 decreased PIK3R2 (p85-β) and enhanced Akt phosphorylation, which resulted in an increase in the survival of CRL-1730 cells exposed to H2O2. More importantly, the downregulation of the miR-126 expression reversed the effects of RSV on the survival and apoptosis of CRL-1730 cells exposed to H2O2. In addition, the knockdown of Ets-1 reversed the effects of RSV on the miR-126 expression in CRL-1730 cells exposed to H2O2. Conclusions:In this study, we demonstrated that the protection of endothelial cells by RSV against oxidative injury is due to the activation of PI3K/Akt by miR-126.

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“…In the cardiovascular system, the reduced expression of SIRT1 is specifically limited to aged and/or atherosclerotic vessels (Kao et al, 2010). Also, it has been demonstrated that RV prevents free radical-induced senescence in human umbilical vein endothelial cells (HUVECs) but fails to protect from oxidative damage when the gene for SIRT1 is silenced (Kao et al, 2010).…”

Section: Considerations For the Use Of Resveratrolmentioning

confidence: 99%

“…For instance, RV has been shown to reverse oxidative damage by reducing the expression of NADPH oxidase while increasing the expression of SIRT1 in the aorta of aged rats (Tang et al, 2012). In the cardiovascular system, the reduced expression of SIRT1 is specifically limited to aged and/or atherosclerotic vessels (Kao et al, 2010). Also, it has been demonstrated that RV prevents free radical-induced senescence in human umbilical vein endothelial cells (HUVECs) but fails to protect from oxidative damage when the gene for SIRT1 is silenced (Kao et al, 2010).…”

Section: Considerations For the Use Of Resveratrolmentioning

confidence: 99%

The effects of resveratrol on aging vessels

Díaz

Degens

Vanhees

et al. 2016

Experimental Gerontology

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Aging is a major risk factor for the development of cardiovascular disease. Despite a significant reduction in the mortality and morbidity rates over the last decade, the socio-economic burden of cardiovascular disease is still substantial. Consequently, there is a considerable need for alternative strategies, such as nutraceutical supplementation, that delay the functional vascular decline present in the elderly.Compromised autophagy and oxidative stress (OS) are considered major causes of the age-related endothelial dysfunction. OS reduces the bioavailability of nitric oxide (NO), which has been associated with hypertension, arteriosclerosis and a reduced vasodilatory response. High levels of free radicals and the low bioavailability of NO lead to a positive feedback loop of further OS, organelle damage, poor repair and endothelial dysfunction. Here we draw attention to the relationship between OS and autophagy in the aged vasculature. We have reviewed the published literature and provided arguments that support that treatment with resveratrol stimulates autophagy and thereby has the potential to restore oxidative balance in the endothelium, which indicates that treatment with resveratrol might have therapeutic potential to restore endothelial function in the elderly.

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Resveratrol Protects Human Endothelium from H2O2-Induced Oxidative Stress and Senescence via SirT1 Activation

Cited by 218 publications

References 44 publications

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Resveratrol Inhibits Hydrogen Peroxide-Induced Apoptosis in Endothelial Cells via the Activation of PI3K/Akt by miR-126

The effects of resveratrol on aging vessels

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