Dietary methionine source and level affect hepatic sulfur amino acid metabolism of broiler breeder hens

Jian-mei, Wan; Ding, Xuemei; Wang, Jianping; Bai, Shiping; Peng, Huanwei; Luo, Yuheng; Su, Zuowei; Xuan, Yue; Zhang, Keying

doi:10.1111/asj.12882

Cited by 13 publications

(13 citation statements)

References 29 publications

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“…When the Met was supplied insufficiently, the levels of Ser and Cys may be altered ( 36 ). At week 16, the lower content of Ala in Met- group was observed, which was consistent with the study by Wan et al ( 37 ). They found that plasma Ala concentration was linearly increased as DL-Met supplementation level increased.…”

Section: Discussionsupporting

confidence: 92%

Effect of Methionine Deficiency on the Growth Performance, Serum Amino Acids Concentrations, Gut Microbiota and Subsequent Laying Performance of Layer Chicks

et al. 2022

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This study was conducted to investigate the effect of methionine (Met) deficiency in the rearing period on the growth performance, amino acids metabolism, intestinal development and gut microbiome of egg-laying chicks and the continuous effects on the performance, egg quality, and serum amino acids metabolism of the subsequent development process. Three hundred sixty one-day-old chicks were randomly divided into two groups and fed on a basal diet (NC group, Met 0.46%) and Met deficiency diet (Met- group, Met 0.27%). Each group included six replicates with 30 chicks per replicate. The trial lasted 6 weeks (0–6 weeks), both groups were fed the same basal diet which met the needs of Met during the observation period (7–24 weeks). Results showed that Met deficiency significantly decreased (P < 0.05) body weight (BW), average daily weight gain (ADG), average daily feed intake (ADFI) and tibia length (TL) compared to the NC group during the trial period (0–6 weeks). Also, Met deficiency dramatically increased (P < 0.05) feed conversion ratio (FCR) during the trial and observation period (7–24 weeks). In addition, during the observation period, the BW and ADG were decreased (P < 0.05) in the Met- group. Moreover, Met- group decreased (P < 0.05) villi height and villi height/crypt depth ratio in jejunum at 6th weeks. In addition, the concentrations of serum main free amino acids (FAA) in the Met- group were significantly increased (P < 0.05) at 6th weeks, while were decreased at 16th weeks. Based on the α-diversity and PCoA analysis in β-diversity, there were no significant differences in the cecal microbial composition between NC and Met- groups. However, the LEfSe analysis revealed that differential genera were enriched in the NC or Met- groups. The Haugh unit, shell thickness and egg production in the Met- group were significantly lower (P < 0.05) than in the NC group. In conclusion, these results revealed that dietary supplementation of appropriate Met could substantially improve the growth performance, host amino acid metabolism and intestinal development and continuously improve the laying performance and thus boost the health of growing hens.

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Section: Discussionsupporting

confidence: 92%

Effect of Methionine Deficiency on the Growth Performance, Serum Amino Acids Concentrations, Gut Microbiota and Subsequent Laying Performance of Layer Chicks

et al. 2022

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“…As one of MAT isomerases, mat2a is essential to modulate catalytic activity of MAT. In the current study, compared to the higher mat2a level in shrimp fed the PC diet, methionine deficiency signals in the NC group caused the reduction of methionine consumption in methylation cycle ( Wan et al, 2017 ). Most marine animals cannot synthesize enough taurine by themselves to fulfil the requirement for healthy growth and development ( Tong et al, 2019 ).…”

Section: Discussionmentioning

confidence: 65%

Comparative study on the effects of crystalline L-methionine and methionine hydroxy analogue calcium supplementations in the diet of juvenile Pacific white shrimp (Litopenaeus vannamei)

et al. 2023

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An 8-week feeding trial was conducted to evaluate the effects of L-methionine and methionine hydroxy analogue calcium (MHA-Ca) supplements in low-fishmeal diet on growth performance, hepatopancreas morphology, protein metabolism, anti-oxidative capacity, and immunity of Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous and isoenergetic diets were designed: PC (203.3 g/kg fishmeal), NC (100 g/kg fishmeal), MET (100 g/kg fishmeal +3 g/kg L-methionine) and MHA-Ca (100 g/kg fishmeal +3 g/kg MHA-Ca). White shrimp (initial body weight 0.23 ± 0.00 g, 50 shrimp per tank) were allocated to 12 tanks and divided among 4 treatments in triplicates. In response to L-methionine and MHA-Ca supplementations, the shrimp exhibited higher weight gain rate (WGR), specific growth rate (SGR), condition factor (CF), and lower hepatosomatic index (HSI) compared to those fed the NC diet (p < 0.05). The WGR and SGR of shrimp fed L-methionine and MHA-Ca showed no difference with those in the PC diet (p > 0.05). Both of L-methionine and MHA-Ca supplementary diets significantly decreased the malondialdehyde (MDA) levels of shrimp when compared with the NC diet (p < 0.05). L-methionine supplementation improved the lysozyme (LZM) activity and total antioxidant capacity (T-AOC) of shrimp, while the MHA-Ca addition elevated the reduced glutathione (GSH) levels in comparison with those fed the NC diet (p < 0.05). Hypertrophied blister cells in hepatocytes were observed in shrimp fed the NC diet, and alleviated with L-methionine and MHA-Ca supplementations. Shrimp fed the MET and MHA-Ca diets had higher mRNA expression levels of target of rapamycin (tor) than those fed the NC diet (p < 0.05). Compared to the NC group, dietary MHA-Ca supplementation upregulated the expression level of cysteine dioxygenase (cdo) (p < 0.05), while L-methionine supplementation had no significant impact (p > 0.05). The expression levels of superoxide dismutase (sod) and glutathione peroxidase (gpx) were significantly upregulated by L-methionine supplemented diet in comparison with those in the NC group (p < 0.05). Overall, the addition of both L-methionine and MHA-Ca elevated the growth performance, facilitated protein synthesis, and ameliorated hepatopancreatic damage induced by plant-protein enriched diet in L. vannamei. L-methionine and MHA-Ca supplements enhanced anti-oxidants differently.

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“…The endogenous AAs were referred to those coming from cellular proteolysis and de novo AA synthesis inside of the body, while the exogenous AAs were referred to those obtained from the dietary supply outside of the body. In laying hens, the increasing methionine supplementation induced a linear increase in plasma concentrations of Ala, Tyr, Val, Gly, and Ser ( Wan, et al., 2017 ), and serum albumin level increased significantly in response to supplemental L-Val ( Azzam, et al., 2015 ). The disappearance of AAs from blood pool was mainly influenced by the absorption from blood by cells for synthesis of new proteins or by utilization as fuel for body energy requirement ( Liao, et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Dynamic variations in serum amino acid and the related gene expression in liver, ovary, and oviduct of pigeon during one egg-laying cycle

Ren

Han

et al. 2021

Poultry Science

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The present study was carried to investigate dynamic variations in serum amino acid ( AA ) contents and the relative mRNA abundance of the AA transporters and AA synthesis-related enzymes in liver, ovary and oviduct of pigeons during one egg-laying cycle ( ELC ). In experiment 1, seventy laying pigeons (American Silver King) were randomly divided into 14 groups by different days of one ELC ( DELC ) and arranged as a 2 × 7 factorial design, which included 2 ages (6-mo-old or 12-mo-old) and 7 DELCs. For experiment 2, 35 six-mo-old laying pigeons (American Silver King) were randomly divided into 7 groups by different DELCs and immediately treated with a 12-h fasting. Dynamic variations in serum AAs were detected during one ELC, characterized by high levels of Lys, Met, Leu, Phe, Tyr, Asp, Ser, Glu, Ala, and TAA on day 1 ( D1 ) of one ELC ( P < 0.05). Fasting caused obvious decreases in serum levels of Leu, Ile, Val, Phe, Tyr, and TAA from day 2 (D2 ) to day 7 ( D7 ) ( P < 0.05). Relative organ weights of ovary and oviduct increased to the peak values on day 13 ( D13 ) ( P < 0.05). Serum calcium decreased to the lowest level on day 4 ( D4 ) ( P < 0.05) and serum total triglyceride was kept in a high level on D1, D7, day 10 ( D10 ), and D13 ( P < 0.05). Relative mRNA expression of the AA synthesis genes and the AA transport genes exhibited different variation patterns in liver, ovary and oviduct, but Pearson correlation test showed the percentage of positive r values with significant differences were much higher in oviduct than those in liver or ovary. In conclusion, dynamic variations of serum AAs during one ELC were positively related with the expression of the AA transport genes and AA synthesis genes in oviduct, suggesting the upregulated serum AAs might be necessary to meet the AAs requirement for egg white formation in pigeon.

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Dietary methionine source and level affect hepatic sulfur amino acid metabolism of broiler breeder hens

Cited by 13 publications

References 29 publications

Effect of Methionine Deficiency on the Growth Performance, Serum Amino Acids Concentrations, Gut Microbiota and Subsequent Laying Performance of Layer Chicks

Effect of Methionine Deficiency on the Growth Performance, Serum Amino Acids Concentrations, Gut Microbiota and Subsequent Laying Performance of Layer Chicks

Comparative study on the effects of crystalline L-methionine and methionine hydroxy analogue calcium supplementations in the diet of juvenile Pacific white shrimp (Litopenaeus vannamei)

Dynamic variations in serum amino acid and the related gene expression in liver, ovary, and oviduct of pigeon during one egg-laying cycle

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