Effect of Methionine Deficiency on the Growth Performance, Serum Amino Acids Concentrations, Gut Microbiota and Subsequent Laying Performance of Layer Chicks

Abstract

This study was conducted to investigate the effect of methionine (Met) deficiency in the rearing period on the growth performance, amino acids metabolism, intestinal development and gut microbiome of egg-laying chicks and the continuous effects on the performance, egg quality, and serum amino acids metabolism of the subsequent development process. Three hundred sixty one-day-old chicks were randomly divided into two groups and fed on a basal diet (NC group, Met 0.46%) and Met deficiency diet (Met- group, Met 0.27%). Each group included six replicates with 30 chicks per replicate. The trial lasted 6 weeks (0–6 weeks), both groups were fed the same basal diet which met the needs of Met during the observation period (7–24 weeks). Results showed that Met deficiency significantly decreased (P < 0.05) body weight (BW), average daily weight gain (ADG), average daily feed intake (ADFI) and tibia length (TL) compared to the NC group during the trial period (0–6 weeks). Also, Met deficiency dramatically increased (P < 0.05) feed conversion ratio (FCR) during the trial and observation period (7–24 weeks). In addition, during the observation period, the BW and ADG were decreased (P < 0.05) in the Met- group. Moreover, Met- group decreased (P < 0.05) villi height and villi height/crypt depth ratio in jejunum at 6th weeks. In addition, the concentrations of serum main free amino acids (FAA) in the Met- group were significantly increased (P < 0.05) at 6th weeks, while were decreased at 16th weeks. Based on the α-diversity and PCoA analysis in β-diversity, there were no significant differences in the cecal microbial composition between NC and Met- groups. However, the LEfSe analysis revealed that differential genera were enriched in the NC or Met- groups. The Haugh unit, shell thickness and egg production in the Met- group were significantly lower (P < 0.05) than in the NC group. In conclusion, these results revealed that dietary supplementation of appropriate Met could substantially improve the growth performance, host amino acid metabolism and intestinal development and continuously improve the laying performance and thus boost the health of growing hens.

Different methionine to cysteine supplementation ratios altered bone quality of broilers with or without Eimeria challenge assessed by dual energy X-ray absorptiometry and microtomography

“…As for the effects of MCR on the whole body compositions, decreased MCR linearly decreased lean tissue weight and BW in the NCG which corresponds with previous studies ( Jariyahatthakij et al, 2018 ; Liu et al, 2022 ) suggesting the importance of Met in body growth cannot be replaced by Cys. While in the CG, partially substituting Met with Cys did not affect the BW and lean tissue weight.…”

“…Methionine ( Met ) is considered as the first limiting amino acid in broilers fed corn soybean meal based diets ( Bunchasak, 2009 ; Attia et al, 2020 ) and maintains the normal growth and functions of the broilers ( Fagundes et al, 2020 ; Liu et al, 2022 ). Cysteine ( Cys ) is considered a semi-essential amino acid since it can be converted from Met ( Castro and Kim, 2020 ).…”

Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction

“… Fagundes et al (2020) assigned 40 broiler chicks into Met-sufficient or Met-deficient groups and found that birds fed with Met-deficient diet had worse growth and feed efficiency. Liu et al (2022) also provided chicks with Met-deficient diet and reported that body weight, weight gain, and feed efficiency were impaired by restricting dietary Met contents. However, in this study, we did not observe that dietary Met restriction had negative effects on BWG, FI, and feed efficiency of broiler chicks ( Table 3 ).…”

Peningkatan Produktivitas Ayam dengan Formulasi Ransum Padat Nutrisi Berbahan Baku DDGS di Lahan Pantai

“…Mutu pakan dipengaruhi kualitas bahan-bahan yang digunakan ransum dan penyimpanan bahanbahan pakan (Tarigan & Manalu, 2019) Penambahan DDGS 30% dengan metionin menurukan konversi hingga 2,01 dibandingkan tanpa pemberian metionin yaitu 2,43 dan 2,15. Penelitian sebelumnya menunjukkan bahwa kekurangan metionin dalam pakan akan menambah FCR baik untuk layer maupun broiler, selain itu akan mempengaruhi kualitas telur (Liu et al, 2022). Penurunan FCR akan berpengaruh pada biaya produksi suatu peternakan, semakin kecil FCR menunjukkan semakin rendah terjadinya pemborosan pakan sehingga keuntungan semakin tinggi.…”