Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes

Streich, Katharina; Smoczek, Margarethe; Hegermann, Jan; Dittrich–Breiholz, Oliver; Bornemann, Melanie; Siebert, Anja; Bleich, André; Büettner, Manuela

doi:10.1016/j.jare.2020.04.020

Cited by 9 publications

(17 citation statements)

References 56 publications

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“…The gut is the primary site for nutrient absorption in all animals and humans ( 1 ). Many single traditional Chinese medicine (TCM), extracts of TCM, and some TCM monomers enter the blood through the intestinal tract after oral administration ( 2 – 4 ). Many parameters affect the intestinal absorption of substances and their bioavailability, such as transit and absorption time ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Uptake and Transport of Naringenin and Its Antioxidant Effects in Human Intestinal Epithelial Caco-2 Cells

et al. 2022

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Naringenin, a flavanone, has been reported for a wide range of pharmacological activities. However, there are few reports on the absorption, transport and antioxidant effects of naringenin. The study was to explore the uptake, transport and antioxidant effects of naringenin in vitro. Cell transmembrane resistance, lucifer yellow transmission rate, and alkaline phosphatase activity were used to evaluate the successful construction of cell model. The results showed that the absorption and transport of naringenin by Caco-2 cells were time- and concentration-dependent. Different temperatures (37 and 4°C) had a significant effect on the uptake and transport of naringenin. Verapamil, potent inhibitor of P-glycoprotein, significantly inhibit naringenin transport in Caco-2 cells. The results revealed that naringenin was a moderately absorbed biological macromolecule and can penetrate Caco-2 cells, mainly mediated by the active transport pathway involved in P-glycoprotein. At the same time, naringenin pretreatment could significantly increase the viability of H2O2-induced Caco-2 cells. Twenty four differential metabolites were identified based on cellular metabolite analysis, mainly including alanine, aspartate and glutamate metabolism, histidine metabolism, taurine and hypotaurine metabolism, pyruvate metabolism, purine metabolism, arginine biosynthesis, citrate cycle, riboflavin metabolism, and D-glutamine and D-glutamate metabolism. We concluded that the transport of naringenin by Caco-2 cells is mainly involved in active transport mediated by P-glycoprotein and naringenin may play an important role in oxidative stress-induced intestinal diseases.

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Section: Introductionmentioning

confidence: 99%

Uptake and Transport of Naringenin and Its Antioxidant Effects in Human Intestinal Epithelial Caco-2 Cells

et al. 2022

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“…The authors describe that under a high-calorie diet in the parenchyma of the lymph nodes increases the proportion of macrophages and stromal cells that contain lipid inclusions. In this study, the authors showed that stromal cells express a large number of genes involved in lipid metabolism, indicating that lymph nodes are involved in lipid metabolism [19].…”

Section: Discussionmentioning

confidence: 71%

Morphometric and Electron Microscopic Changes of Structural Components of Lymph Nodes During Correction of the Action of Sodium Glutamate With Orlistat

Harapko

Матешук-Вацеба

2021

Wiad Lek

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The aim: To study morphometric and electron microscopic changes in the parenchyma of rat lymph nodes under the action of sodium glutamate and its correction by orlistat. Materials and methods: The article presents and analyzes the data of an experimental study conducted on 66 white male rats and females of reproductive age. Experimental animals are divided into 4 groups. Results: After six weeks of exposure to monosodium glutamate, there was a significant decrease in the relative area of the cortical substance in the lymph nodes of white male and female rats by 11.95% and 9.31% and, respectively, an increase in the relative area of the medullary substance by 18.76% and 14.7% in compared with an intact group of animals. After six weeks of sodium glutamate and the next six weeks of the standard diet of vivarium and orlistat, the relative area of the cortical substance in the lymph node parenchyma was 2.55% and 0.38% more than the parameters of the intact group of animals, respectively. Accordingly, the relative area of the medullary substance decreased and was 4.01% and 0.59% less compared to the intact group of animals. Conclusions: Electron microscopic examination showed that monosodium glutamate causes changes in the parenchyma of the lymph nodes as in a high-calorie diet. The introduction of orlistat (xenical) leads to a partial restoration of the structural organization, and hence the function of this organ.

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“…Moreover, transmission electron microscopy enabled the identification of lipid droplets in lymphatic endothelial cells, different reticulum cells, and macrophages, and the lipid droplet sizes, as well as their numbers and intercellular distances, increased after 10 weeks of high-fat diet feeding mice [ 115 ]. The results indicate that changes in the microarchitecture and increased accumulation of lipid droplets in stromal cells and macrophages influence the immunological function of the microarchitecture of the mesenteric lymph node [ 115 ].…”

Section: Lymphatic System—physiology and Functionsmentioning

confidence: 99%

Adipose Tissue and Biological Factors. Possible Link between Lymphatic System Dysfunction and Obesity

et al. 2021

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The World Health Organization (WHO) has recognised obesity as one of the top ten threats to human health. Obesity is not only a state of abnormally increased adipose tissue in the body, but also of an increased release of biologically active metabolites. Moreover, obesity predisposes the development of metabolic syndrome and increases the incidence of type 2 diabetes (T2DM), increases the risk of developing insulin resistance, atherosclerosis, ischemic heart disease, polycystic ovary syndrome, hypertension and cancer. The lymphatic system is a one-directional network of thin-walled capillaries and larger vessels covered by a continuous layer of endothelial cells that provides a unidirectional conduit to return filtered arterial and tissue metabolites towards the venous circulation. Recent studies have shown that obesity can markedly impair lymphatic function. Conversely, dysfunction in the lymphatic system may also be involved in the pathogenesis of obesity. This review highlights the important findings regarding obesity related to lymphatic system dysfunction, including clinical implications and experimental studies. Moreover, we present the role of biological factors in the pathophysiology of the lymphatic system and we propose the possibility of a therapy supporting the function of the lymphatic system in the course of obesity.

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Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes

Cited by 9 publications

References 56 publications

Uptake and Transport of Naringenin and Its Antioxidant Effects in Human Intestinal Epithelial Caco-2 Cells

Uptake and Transport of Naringenin and Its Antioxidant Effects in Human Intestinal Epithelial Caco-2 Cells

Morphometric and Electron Microscopic Changes of Structural Components of Lymph Nodes During Correction of the Action of Sodium Glutamate With Orlistat

Adipose Tissue and Biological Factors. Possible Link between Lymphatic System Dysfunction and Obesity

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