Dietary effect of mead acid on DMBA‑induced breast cancer in female Sprague‑Dawley rats

Abstract

In the present study, the dietary effects of mead acid (MA; 5,8,11-eicosatrienoic acid) on 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer in female Sprague-Dawley rats were examined. The 2.4 and 4.8% MA diets were commenced when the rats were 6 weeks of age. DMBA was administered by a single oral ingestion when the rats were 7 weeks of age, and the rats were maintained on the respective diets until 19 weeks of age. Tumor weight, histopathology, cell kinetics, and the fatty acid composition in breast tissue and serum were examined. In the control (CTR) group, the DMBA-exposed rats were fed a basal diet (0% MA). The results revealed that there were no significant differences in tumor incidence, cell kinetics and in the N-6/N-3 ratio in breast tissue between the groups. Only the N-6/N-3 ratio of fatty acid composition in serum was significantly decreased in the 2.4% MA diet group. In previous studies, the 2.4% MA diet was shown to suppress N-methyl-N-nitrosourea-induced luminal A mammary cancer by decreasing cancer cell proliferation. The findings of the present study differ from those of previous studies with different breast cancer models. To further clarify the effects of MA against breast carcinogenesis, further investigations with different experimental breast cancer models are recommended.

Histopathological study of the protective effect of vitamin E against DMBA induced mammary cancer in rats

“…Since DMBA changed the standard biochemical parameters of the animal body, altered antioxidants enzymes activities, increased reactive oxygen species (ROS) & lipid peroxidation which resulted in damaging the cellular structure and membrane of organelles including: DNA, lipids & proteins as represented by Rojas-Armas et al [16]. This result agreed with Kinoshita et al & Rojas-Armas et al [16,17].…”

Effect of Eleuhterine Palmifolia (L) Merr on Tumor Cell Proliferation in Sprague Dawley Rats With Breast Cancer

“…Jenis tikus dipilih karena berkaitan dengan sifatnya yang hormonaldependent sehingga sensitif terhadap rangsang dimetylbenz (α) antrasene (DMBA). 14,15 Berdasarkan penelitian sebelumnya, tingkat keberhasilan induksi DMBA secara oral adalah sebesar 70%, sehingga peneliti melakukan induksi DMBA terhadap 27 ekor tikus Sprague-Dawley. Setelah aklimatisasi selama 1 minggu, tikus diinduksi DMBA.…”

The physiological and pathological properties of Mead acid, an endogenous multifunctional n-9 polyunsaturated fatty acid

“…The initiation and promotion phases of mammary carcinogenesis in N -methyl- N -nitrosourea-induced cancer model in rats were also suppressed upon the administration of MA [ 36 ]. On the other hand, MA administration did not suppress 7,12‑dimethylbenz[a]anthracene‑induced breast cancer in rats [ 72 ]. Different results depending on the carcinogens were reported.…”