Dielectrophoresis for Bioparticle Manipulation

Qian, Cheng; Huang, Haibo; Chen, Liguo; Li, Xiangpeng; Ge, Zunbiao; Chen, Tao; Yang, Zhan; Sun, Lining

doi:10.3390/ijms151018281

Cited by 153 publications

(133 citation statements)

References 126 publications

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“…Moreover, in a previous study, we demonstrated that the method that we proposed based on negative DEP has high sensitivity that enables detection of as little as 80 molecules, which corresponds to 13 attomolars, per PM combined with the high specificity of an immunoassay with monoclonal antibodies [15,16]. DEP is a force produced by an electric field gradient in dielectric particles [17][18][19][20][21]. Negative DEP is a force produced by an electric field gradient to dielectric particles that is opposite to the direction of the electric field gradient.…”

Section: Introductionmentioning

confidence: 98%

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

et al. 2018

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Abstract:We show that negative dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism of a biosensor for the diagnosis and prognosis of pancreatic cancer using the biomarker CA 19-9. A substantial change in the negative DEP force applied to functionalized polystyrene microspheres (PM) was observed with respect to both the concentration level of the pancreatic cancer biomarker CA 19-9 and the frequency of the electric field produced by a pearl shaped interdigitated gold micro-electrode. The velocity of repulsion of a set of PM functionalized to a monoclonal antibody to CA 19-9 was calculated for several concentration cutoff levels of CA 19-9, including 0 U/mL and 37 U/mL, at the frequency range from 0.5 to 2 MHz. The velocity of repulsion of the PM from the electrode was determined using a side illumination and an automated software using a real-time image processing technique that captures the Mie scattering from the PM. Since negative DEP spectroscopy is an effective transduction mechanism for the detection of the cutoff levels of CA 19-9, it has the potential to be used in the early stage diagnosis and in the prognosis of pancreatic cancer.

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Section: Introductionmentioning

confidence: 98%

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

et al. 2018

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“…Since the dielectrophoretic force is proportional to the electric field gradient, it can appear either when direct current (DC) or alternate current (AC) is applied. The non-uniform electric field can be generated using an array of electrodes (electrode based, eDEP) [5][6][7] or an array of insulating posts (insulator based, iDEP).…”

Section: Introductionmentioning

confidence: 99%

A new approach to design an efficient micropost array for enhanced direct-current insulator-based dielectrophoretic trapping

et al. 2016

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Aquesta és una còpia de la versió author's final draft d'un article publicat a la revista Analytical and bioanalytical chemistry.La publicació final està disponible a Springer a través de http://dx.doi.org/10.1007/s00216-016-9629-2 This is a copy of the author 's final draft version of an article published in the journal Analytical and bioanalytical chemistry. AbstractDirect-current insulator-based dielectrophoresis (DC-iDEP) is a well-known technique that benefits from the electric field gradients generated by an array of insulating posts to separate or trap biological particles. In this work, we propose a novel figure of merit to find an efficient design of the post-array distribution in a microfluidic channel. To characterization can be used to reduce the required electric field to achieve effective particle trapping and, therefore, avoid the negative effects of Joule heating in cells or viable particles.

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“…[11][12][13][14][15][16] In recent years, studies of microelectrodebased DEP fluidic device for cell separation have rapidly become more popular and attracted much attention due to advanced micro-processing technologies. 17 A cell sorting technique using a combination of platinum electrodes with a photo-patterned insulator was proposed to analyze and synchronize yeast cell division. 18 Polymeric microfluidic device with integrated thick Carbon-Polydimethylsiloxane (C-PDMS) composite electrodes was proposed to carry out DEP trapping of low abundance biological cells.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of continuous-flow separation of viable/nonviable yeast cells using a nonuniform alternating current electric field with complex spatial distribution

et al. 2016

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The variability in cell response to AC electric fields is selective enough to separate not only the cell types but also the activation states of similar cells. In this work, we use dielectrophoresis (DEP), which exploits the differences in the dielectric properties of cells, to separate nonviable and viable cells. A parallel-plate DEP device consisting of a bottom face with an array of micro-fabricated interdigitated electrodes and a top face with a plane electrode was proposed to facilitate the separation of cells by creating a nonuniform electric field throughout the flow channel. The operation and performance of the device were evaluated using live and dead yeast cells as model biological particles. Further, numerical simulations were conducted for the cell suspensions flowing in a channel with a nonuniform AC electric field, modeled on the basis of the equation of motion of particles, to characterize the separation efficiency by changing the frequency of applied AC voltage. Results demonstrated that dead cells traveling through the channel were focused onto a site around the minimum electric field gradient in the middle of the flow stream, while live cells were trapped on the bottom face. Cells were thus successfully separated under the appropriately tuned frequency of 1 MHz. Predictions showed good agreement with the observation. The proposed DEP device provides a new approach to, for instance, hematological analysis or the separation of different cancer cells for application in circulating tumor cell identification. Published by AIP Publishing.

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Dielectrophoresis for Bioparticle Manipulation

Cited by 153 publications

References 126 publications

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

Label-Free Biosensing Method for the Detection of a Pancreatic Cancer Biomarker Based on Dielectrophoresis Spectroscopy

A new approach to design an efficient micropost array for enhanced direct-current insulator-based dielectrophoretic trapping

Enhancement of continuous-flow separation of viable/nonviable yeast cells using a nonuniform alternating current electric field with complex spatial distribution

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