Spectroscopic and tomographic studies on the solid tissues of a human tooth have been carried out in the terahertz (THz) frequency range. It is experimentally shown that, in samples of dental tissue damaged by caries, the absorption lines of water in the THz region have higher intensity by comparison with the intensity of healthy tissue. The radiation intensity in the THz frequency range reflected from samples of human dental enamel damaged by caries in this case is smaller by a factor of 2.2 and from samples of dentine is smaller by a factor of 4.5 by comparison with samples of healthy tissue.
INTRODUCTIONTerahertz (THz) pulse spectroscopy and tomography are relatively new methods that have been used in both technical 1,2 and biological 3,4 applications. This is because many substances have definite absorption lines in the THz frequency range that can be used to identify the materials. For example, vibrational, rotational, and conformational modes associated with protein and DNA molecules appear in this frequency range, and this makes it possible to estimate the status of a biological object. 3 If one simultaneously uses information concerning the reflection spectra of a substance and concerning the temporal shapes of the reflected pulses in the THz region, it is possible to obtain an image that allows one to determine the internal structure and composition of an object composed of several layers of dielectrics. 5,6 As has been mentioned, THz radiation can be used for biomedical diagnosis 1,7-15 -in particular, to diagnose carious damage of teeth. [9][10][11][12][13][14][15] Caries is a widespread disease, and therefore early detection of latent caries is very important, since timely intervention can prevent its development at an early stage. There is no practical technology in stomatology for obtaining an objective three-dimensional image for the analysis of defects of dental enamel. 16 The development of a method of nondestructive diagnosis of enamel is a crucial problem. 17,18 There are methods of THz tomography that make it possible to obtain a tomographic map of teeth. 8-15 Tomographic images, as a rule, are formed by point-by-point recording of the temporal shapes of a THz pulse reflected from the test object. 9 At present, most studies have been carried out on dry sections of teeth; however, there are studies for complete teeth with a water content that corresponds to the moisture level of the oral cavity. 9,10 This paper reports studies of the solid tissues of the tooth, using spectroscopy and tomography in the THz frequency range.
EXPERIMENTAL APPARATUSAn original laboratory prototype of THz reflectometric tomography (Fig. 1) developed earlier 19 was used to investigate the internal structure of the hard tissues of a tooth. In the optical layout of the laboratory prototype, a laser beam from an FL-1 femtosecond laser (FL) by means of beamsplitter plate M 1 is divided into a probe beam and a pump beam. A system of mirrors (M 7 , M 9 , M 10 ) is used to direct the probe beam through half-wave plate λ∕2 an...