Die Inhaltsstoffe des normalen Stratum corneum und Callus menschlicher Haut

Jacobi, Otto

doi:10.1007/bf00496555

Cited by 12 publications

(3 citation statements)

References 17 publications

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“…We chose peptone, keratin and lipids as nutrients for 1. rubrum, because biochemically similar substrates are available for the fungus during its parasitic growth in human skin [6]. In addition, no relevant amounts of glucose are known to be found in the epidermis [7]. Since enzymes liberated by the invading fungi can be assumed to be particularly prone to interact with host tissue, we decided to perform a screening for enzymes released into the environment under these different nutritional conditions.…”

Section: Discussionmentioning

confidence: 99%

“…This is accentuated even more by the pronounced suppressive effect of glucose feeding on the enzyme release by 7. rubrum. Although not a component of normal skin [7], glucose is an ingredient of many common mycological growth media, including Sabouraud's glucose agar as the standard medium. We suggest that glucose-containing substrates are not suitable for growing dermatophytes for the purpose of enzyme studies with pathogenetic relevance.…”

Section: Discussionmentioning

confidence: 99%

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Enzyme release by Trichophyton rubrum depends on nutritional conditions

Brasch

Martins

Christophers

1991

Mycoses

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Summary. Enzymes liberated by growing der‐matophytes are of pathogenetic importance in tinea. To investigate the influence of nutrients on this enzyme release, Trichophyton rubrum was grown in media containing peptone, keratin and lipids, to which glucose was added in separate assays. The culture supernatants were compared for extracellular enzyme activities by use of the api‐zym® test. Our results clearly show that the extracellular enzyme activity is dependent on the nutrients supplied. Seven different enzymes were released when keratin was supplied, as compared with only five and two respectively when lipids or peptone were available. Among these enzymes alkaline phosphatase and N‐acetyl‐β‐glucosaminidase were detected in all cultures lacking glucose. Enzyme release was inhibited completely when glucose was added to the media, except for N‐acetyl‐β‐glucosaminidase in peptone cultures. This dependency of enzyme release on fungal nutrition can be expected to occur in vivo too. In addition, it has to be considered for in vitro cultural conditions. Alkaline phosphatase and acetylglucosaminidase may be more important in tinea than has been assumed so far. Zusammenfassung. Von Dermatophyten frei‐gesetzte Enzyme sind bei der Entstehung einer Tinea pathogenetisch relevant. Um den Einfluβ von Nährsubstraten auf die Enzymfreisetzung von Trichophyton rubrum zu untersuchen, wurde dieser Erreger in pepton‐, keratin‐ und lipid‐haltigen Nährmedien gezüchtet, denen in zusätz‐lichen Ansätzen Glucose beigefügt wurde. Die Kulturüberstände wurden mit dem api‐zym® ‐Test auf extrazelluläre Enzymaktivität unter‐sucht. Unsere Ergebnisse zeigen eine eindeutige Abhängigkeit dieser Enzymaktivitäten vom Nährstoffangebot. Bei Wachstum auf Keratin wurden sieben verschiedene Enzyme bestimmt, bei Lipid‐ bzw. Peptonangebot dagegen nur fünf bzw. zwei. Alkalische Phosphatase und N‐Acetyl‐β‐Glucosaminidase waren in alien glucosefreien Nährmedien nachweisbar. Durch Glucosezusatz zu den Nährmedien erfolgte eine vollständige Hemmung der Enzymfreisetzung mit Ausnahme von N‐Acetyl‐β‐Glucosaminidase in der Pepton‐kultur. Diese Nährstoffabhangigkeit der Enzymfreisetzung kann auch in vivo vermutet werden und ist bei der Auswahl von Kulturmedien zu berücksichtigen. Alkalische Phosphatase und Acetylglucosaminidase könnten mehr Bedeutung bei Dermatophytosen besitzen als bisher ange‐nommen.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Enzyme release by Trichophyton rubrum depends on nutritional conditions

Brasch

Martins

Christophers

1991

Mycoses

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“…Water-soluble substances were extracted from the following horny tissues: (1) psoriatic scales collected from non-treated lesions of two patients with psoriasis vulgaris, a man aged 46 years and a woman aged 68 years, and exfoliated scales from the bed sheets of an ii-year-old girl with pustular psoriasis at the time of exacerbation; (2) exfoliated scales from the bed sheets of two patients with exfoliative dermatitis of non-psoriatic origin, a 68-year-oId man and a 46-year-old woman; and (3) pooled samples of callus and clavus obtained from healthy volunteers and patients with psoriasis vulgaris. Although the chemical composition of normal stratum corneum differs slightly from that of callus (Fisher, 1965;Jacobi, 1971), in a preliminary study it was noted that the callus extract and an extract of epidermal sheets prepared from hypertrophic scars obtained at surgery showed a similar chemotactic behaviour. Thus callus was used in order to obtain sufficient amounts of orthokeratotic homy layers instead of normal horny layers.…”

Section: Extracts Of Horny Tissuesmentioning

confidence: 99%

Leukotactic properties of soluble substances inpsoriasis scale

1976

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In an attempt to elucidate the mechanisms underlying the production of transepidermal migration of leukocytes toward the stratum corneum in psoriatic lesions, the chemotactic properties of soluble substances in psoriasis scales were examined by a modified Boyden's chamber. All crude extracts of horny tissues studied, i.e. callus, scales of exfoliative dermatitis and of psoriasis vulgaris, showed chemotactic activity for human peripheral blood leukocytes. But only the chemotactic activity of the psoriasis scale extract was highly potent. This was greatly reduced after dialysis. Fresh serum was not required to manifest the chemotactic activity. By Sephadex G-200 chromatography, the scale extracts from psoriasis vulgaris and pustular psoriasis had potent activity eluted near the cytochrome C marker. The same fractions of other horny tissue extracts, bacterial filtrate prepared from cultured psoriasis scale fragments, and serum did not show such potent activity. On the basis of analysis by gel filtration and recent findings of immunopathological studies, a postulate was made that a complement derived chemotactic factor, possibly a C5 cleavage product, developed as a result of antigen-antibody reaction in the stratum corneum of psoriatic lesions.

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Arbeitsphysiologie der Hornschicht in Grundzügen

Klaschka

1979

Normale Und Pathologische Physiologie Der Haut II

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Die Inhaltsstoffe des normalen Stratum corneum und Callus menschlicher Haut

Cited by 12 publications

References 17 publications

Enzyme release by Trichophyton rubrum depends on nutritional conditions

Enzyme release by Trichophyton rubrum depends on nutritional conditions

Leukotactic properties of soluble substances inpsoriasis scale

Arbeitsphysiologie der Hornschicht in Grundzügen

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