Leukotactic properties of soluble substances inpsoriasis scale

Abstract

In an attempt to elucidate the mechanisms underlying the production of transepidermal migration of leukocytes toward the stratum corneum in psoriatic lesions, the chemotactic properties of soluble substances in psoriasis scales were examined by a modified Boyden's chamber. All crude extracts of horny tissues studied, i.e. callus, scales of exfoliative dermatitis and of psoriasis vulgaris, showed chemotactic activity for human peripheral blood leukocytes. But only the chemotactic activity of the psoriasis scale extract was highly potent. This was greatly reduced after dialysis. Fresh serum was not required to manifest the chemotactic activity. By Sephadex G-200 chromatography, the scale extracts from psoriasis vulgaris and pustular psoriasis had potent activity eluted near the cytochrome C marker. The same fractions of other horny tissue extracts, bacterial filtrate prepared from cultured psoriasis scale fragments, and serum did not show such potent activity. On the basis of analysis by gel filtration and recent findings of immunopathological studies, a postulate was made that a complement derived chemotactic factor, possibly a C5 cleavage product, developed as a result of antigen-antibody reaction in the stratum corneum of psoriatic lesions.

Interleukin-1-Like Activity in Horny Layer Extracts: Decreased Activity in Scale Extracts of Psoriasis and Sterile Pustular Dermatoses

“…Scales were also collected from 1 case of pityriasis rubra pilaris. Eight samples of orthokcratotic homy tis sues were also obtained from noninflammatory skin such as normal skin of an amputated leg (1), normal plantar homy layer ( 1 ), plantar callus (3), ichthyosis vul garis (1), X-linked ichthyosis (1), and hypertrophic scar (1). These samples were stored at -70"C.…”

“…Scale extracts prepared from psoriasis or related dermatoses characterized by the formation of sterile subcorneal pustules such as pustu losis palmaris et plantaris and subcorneal pustular dermatosis, contain various che motactic factors responsible for PMN chemotaxis into the lesional epidermis [1][2][3][4][5][6]. It is just like the stratum of rock that contains various historical remnants.…”

The Pustule in Palmoplantar Psoriasis: Transformed Vesicle or Mature Microabscess?

“…These forces could explain why the vesicles studied were found at the oblique slopes of the rctc ridges and why none were found directly beneath the furrow or the crest. Additionally, in psoriasis leukotactic factors are detectable in the stratum corneum [22][23][24][25], resulting in an immigration of granulocytes. These migrating cells finally form the Munro microabscess.…”

The major internal protein, p27, of a retrovirus-like particle participates in immune complex formation in psoriasis

“…Humoral antibodies have been demonstrated in psoriatic lesions and considerable amounts of IgG and IgA can be extracted from psoriatic scales (17). The concordant formation of immune complexes is indicated by the presence of complement factors (17) and chemotactic factors in psoriatic scales (22,23).…”