Die Gewinnung, Haltung und Aufzucht keimfreier Tiere und ihre Bedeutung für die Erforschung natürlicher Lebensvorgänge

Küster,

doi:10.1007/978-3-662-26235-1

Cited by 3 publications

(3 citation statements)

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“…Sheep and goats have been reared as first generation germ-free animals to approximately 4 months of age (299), following an older report (164).…”

Section: The Animal Host: Germ-free Ormentioning

confidence: 99%

“…However, the recognition of the need to work with pure systems in biological experimentation, from which Pasteur appears to have drawn his analogy [quoted by Ducluzeau (71)], can be traced further back to the experiments of Boussingault (33) on nitrogen fixation of plants grown in a "sterile" soil which were carried out during the first half of the past century. Various reviews on gnotobiotic experimentation (e.g., 164,178) have commented in detail on Pasteur's keynotes of our subject. It is less well known that Schottelius, a pioneer in the area of gnotobiotics who in his younger years spent some time in Pasteur's laboratory, has in one of his papers (290) expanded the master's scanty published remarks.…”

Section: Introductionmentioning

confidence: 99%

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The gnotobiotic animal as a tool in the study of host microbial relationships.

Gordon¹,

Pesti²

1971

Bacteriological Reviews

270

121

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“…Sheep and goats have been reared as first generation germ-free animals to approximately 4 months of age (299), following an older report (164).…”

Section: The Animal Host: Germ-free Ormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The gnotobiotic animal as a tool in the study of host microbial relationships.

Gordon¹,

Pesti²

1971

Bacteriological Reviews

270

121

View full text Add to dashboard Cite

“…1850s watchmakers were the first to implement techniques to prevent dirt in their factory (Holbrook 2010) and in 1885, workers interested in germ-free life developed many types of mechanical barrier or isolation systems (Briggs Phillips 1965). In 1915 the first controlled environment was documented (Kuster 1915). It describes an area, with entrance air locks and air filters, that operates under positive pressure for the breading of germ-free goats.…”

mentioning

confidence: 99%

Cleanrooms and tissue banking how happy I could be with either GMP or GTP?

et al. 2013

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The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guidelines for tissue banking. Based on the experience of the participating centers, the results of the monitoring program were evaluated to determine the feasibility of a cleanroom in tissue banking and the monitoring program. Also the microbial efficacy of a laminar airflow cabinet and an incubator in a cleanroom environment was evaluated. This study indicated that a monitoring program of a cleanroom at rest in combination with (final) product testing is a feasible approach. Although no statistical significance (0.90 \ p \ 0.95) was found there is a strong indication that a Grade D environment is not the ideal background environment for a Grade A obtained through a laminar airflow cabinet. The microbial contamination of an incubator in a cleanroom is limited but requires closed containers for tissue and cell products.

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Sterility testing of germ-free mouse colonies

Dremova,

Mimmler,

Paeslack

et al. 2023

Front. Immunol.

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In biomedical research, germ-free and gnotobiotic mouse models enable the mechanistic investigation of microbiota-host interactions and their role on (patho)physiology. Throughout any gnotobiotic experiment, standardized and periodic microbiological testing of defined gnotobiotic housing conditions is a key requirement. Here, we review basic principles of germ-free isolator technology, the suitability of various sterilization methods, and the use of sterility testing methods to monitor germ-free mouse colonies. We also discuss their effectiveness and limitations, and share the experience with protocols used in our facility. In addition, possible sources of isolator contamination are discussed and an overview of reported contaminants is provided.

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Die Gewinnung, Haltung und Aufzucht keimfreier Tiere und ihre Bedeutung für die Erforschung natürlicher Lebensvorgänge

Cited by 3 publications

References 0 publications

The gnotobiotic animal as a tool in the study of host microbial relationships.

The gnotobiotic animal as a tool in the study of host microbial relationships.

Cleanrooms and tissue banking how happy I could be with either GMP or GTP?

Sterility testing of germ-free mouse colonies

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