Sterility testing of germ-free mouse colonies

Dremova, Olga; Mimmler, Maximilian; Paeslack, Nadja; Khuu, My Phung; Gao, Zhenling; Bosmann, Markus; Garo, Lucien P.; Schön, Nathalie; Mechler, Alexa; Beneich, Yunes; Rebling, Vivian; Mann, Amrit; Pontarollo, Giulia; Kiouptsi, Klytaimnistra; Reinhardt, Christoph

doi:10.3389/fimmu.2023.1275109

Cited by 2 publications

(1 citation statement)

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“…In spite of many applications of GF technology, it has not been widely implemented outside the laboratory yet. The main reason is that GF modeling is a labor-intensive technology that requires constant control to manage the state of cleanliness [68] – [70] . However, the existing methodology underlying the production of GF animals has remained essentially unchanged.…”

Section: History Of Gf Animal Modelingmentioning

confidence: 99%

What we need to know about the germ-free animal models

Aghighi,

Salami

2024

AIMSMICRO

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<abstract> <p>The gut microbiota (GM), as a forgotten organ, refers to the microbial community that resides in the gastrointestinal tract and plays a critical role in a variety of physiological activities in different body organs. The GM affects its targets through neurological, metabolic, immune, and endocrine pathways. The GM is a dynamic system for which exogenous and endogenous factors have negative or positive effects on its density and composition. Since the mid-twentieth century, laboratory animals are known as the major tools for preclinical research; however, each model has its own limitations. So far, two main models have been used to explore the effects of the GM under normal and abnormal conditions: the isolated germ-free and antibiotic-treated models. Both methods have strengths and weaknesses. In many fields of host-microbe interactions, research on these animal models are known as appropriate experimental subjects that enable investigators to directly assess the role of the microbiota on all features of physiology. These animal models present biological model systems to either study outcomes of the absence of microbes, or to verify the effects of colonization with specific and known microbial species. This paper reviews these current approaches and gives advantages and disadvantages of both models.</p> </abstract>

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Section: History Of Gf Animal Modelingmentioning

confidence: 99%