Die DC- and HPLC-Analyse der Eleutherococcus Droge

Abstract

A rapid and reproducible TLC- and HPLC-analysis of root-extracts and preparations of ELEUTHEROCOCCUS SENTICOSUS on the basis of the characteristic lignans and other phenylpropan derivatives has been developed.

Flavonoids fromInula viscosa

“…On acetylation 15 and 16 gave the same tetracetyl derivative, so 16 was identified as 3-O-acetylpadmatin, which was for the first time isolated as a natural compound. From the same extract two sesquiterpene lactones, identified as inuviscolide and 2-deacetoxycanthinin (5), ferulaldehyde (32) and 2-methylphloroacetophenone (33) were also isolated. …”

The chromatographic co‐elution of dihydrodehydrodiconiferyl alcohol monopyranose with eleutheroside E in Eleutherococcus senticosus: implications for eleutheroside E assays

“…The aim of the isolation procedure was to isolate eleutheroside B and E, presumed to correspond to peaks 2 and 3 ( Fig. 3) on analytical HPLC on the basis of published chromatograms (Wagner et al, 1982;Slacanin Analytical HPLC chromatogram of a root extract of Eleutherococcus senticosus (1.811 mg/mL). Key to peak identity: 1, internal standard (3,5-dihydroxybenzyl alcohol) (0.00491 mg/mL); 2, eleutheroside B; 3, dihydrodehydrodiconiferyl alcohol monopyranose.…”

“…1 H-NMR spectra for eleutheroside E (Addipharma, Hamburg, Germany) were obtained in dimethylsulphoxide et al, 1991). After performing solvent extraction and flash chromatographic procedures based on those used by Wagner et al (1982), the butanolic phase was subjected to preparative HPLC using three preparative Nova-Pak ® (Waters, Milford, MA, USA) reverse-phase C 18 columns (100 × 40 mm i.d. ; 125 Å; 15-20 µm) connected in series, and an acetonitrile:water mobile phase maintained at 10% acetonitrile for 10 min after sample injection and then rising to 27% acetonitrile over the next 30 min.…”

The isolation of eleutherococcus senticosus constituents by centrifugal partition chromatography and their quantitative determination by high performance liquid chromatography

“…fraxidin, (-)-syringaresinol, caffeic acid, caffeic acid ethyl ester, coniferylaldehyde, sinapyl alcohol, psitosterol, oleanolic acid (Wagner et al, 1982;Farnsworth et al, 1985). It is claimed that eleutheroside E has the most pronounced stimulant and antistress effects of all the E. senticosus glycosides tested (Breckhman and Dardymov, 1969).…”

“…senticosus constituents, some work has appeared on the spectrophotometric or fluorimetric determination of mixtures of the glycosidic components (Lapchik et al, 1969;Solov'eva et al, 1989) or of the individual glycosides after scraping bands off thin layer chromatographic (TLC) plates (Baevskii et al, 1982). Preliminary investigations of TLC and high performance liquid chromatographic (HPLC) conditions for the qualitative and quantitative determination of certain extracts of different origin were carried out (Wagner and Wurmbock, 1977;Wagner et al, 1982;Vanhaelen and Vanhaelen-Fastre, 1984) and very recently a more detailed TLC and HPLC investigation of E . senticosus and other Acanthopanax species has been reported (Bladt et af., 1990).…”