Die Bedeutung der Hydrolyse-Art in der Feulgen-Cytophotometrie von Kernen mit unterschiedlichen Ploidiegraden

Vahs, Wilfried

doi:10.1007/bf00306263

Cited by 12 publications

(1 citation statement)

References 10 publications

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“…Medium renewal after 1 day. After a total incubation time of 3 days, the cultures were fixed in mcthanol/formol/glacial acetic acid (85/10/5) [Sprenger and Böhm, 1971], The hydrolysis was performed for I It in 5 n HCI [Vahs, 1973] followed by selective nuclear staining with pararosaniline (Fculgcn reaction) according to G raumann [1953], For the measurement of nuclear area with the electronic image analyzer Quantimct (Imaneo, Metals Research) we used photographs of the preparations (enlargement 350:1). This was portrayed on the monitor with a Vidikon TV tube (objective 32 mm).…”

Section: Methodsmentioning

confidence: 99%

Influence of cell density of nuclear size in monolayer cells from embryonic mouse brains

Zimmermann¹,

Adhami²

1976

Cells Tissues Organs

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Studies were carried out on the nuclear size of embryonic mouse brain monolayer cells at various cell densities. The variation in cell density resulted from different initial cell numbers. The nuclear area was measured area-analytically with the Quantimet, the determination of cell population was measured linear-analytically using the Digiscan. Nuclear size remains constant in a cell culture of 10⁴ up to 8.3 × 10⁴ cells ml, which corresponds to a cell density of about 25–160 cells/unit. A significant diminution in mean nuclear size of the cell nucleus occurs in the case of increased cell densities of 215 and 277 cells/unit, corresponding to a cell culture of 2.5 × 10⁵ and 7.3 × 10⁵ cells ml. This diminution may be connected with the accumulation of Gl cell nuclei in cultures with contact-dependent growth inhibition.

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Section: Methodsmentioning

confidence: 99%

Influence of cell density of nuclear size in monolayer cells from embryonic mouse brains

Zimmermann¹,

Adhami²

1976

Cells Tissues Organs

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Air drying as a preparatory factor in cytology: Investigation of its influence on dye uptake and dye binding

Schulte

1986

Diagnostic Cytopathology

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The effect of air-drying on cytological material is investigated in this article. Smears of rat liver were fixed completely wet and, after air drying, postfixed in ethanol, methanol/formaldehyde/acetic acid (MFA), and formaldehyde. Staining was performed with the thionin-Feulgen procedure, a standard Romanowsky-Giemsa stain with azure B-eosin Y and a Papanicolaou staining variant. The image analysis system IBAS 2000 was applied to evaluate objective criteria of the changes caused by air drying the chromatin texture. Nuclear absorption was measured with a Vickers M 85a Microdensitometer. Air-drying had striking effects on size and shape of cell nuclei (spreading), on the structure of the nuclear chromatin (chromatin condensation), and on the chromaticity coordinates (hue, saturation, and intensity of nuclear staining). The variations of the chromatin texture and dye-substrate affinity are attributed to alterations of the tertiary structure of the nuclear proteins.

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Condensed Chromatin: Species-Specificity, Tissue-Specificity, and Cell Cycle-Specificity, as Monitored by Scanning Cytometry

Nagl

1982

Cell Growth

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Die Bedeutung der Hydrolyse-Art in der Feulgen-Cytophotometrie von Kernen mit unterschiedlichen Ploidiegraden

Cited by 12 publications

References 10 publications

Influence of cell density of nuclear size in monolayer cells from embryonic mouse brains

Influence of cell density of nuclear size in monolayer cells from embryonic mouse brains

Air drying as a preparatory factor in cytology: Investigation of its influence on dye uptake and dye binding

Condensed Chromatin: Species-Specificity, Tissue-Specificity, and Cell Cycle-Specificity, as Monitored by Scanning Cytometry

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