Influence of cell density of nuclear size in monolayer cells from embryonic mouse brains

Abstract

Studies were carried out on the nuclear size of embryonic mouse brain monolayer cells at various cell densities. The variation in cell density resulted from different initial cell numbers. The nuclear area was measured area-analytically with the Quantimet, the determination of cell population was measured linear-analytically using the Digiscan. Nuclear size remains constant in a cell culture of 10⁴ up to 8.3 × 10⁴ cells ml, which corresponds to a cell density of about 25–160 cells/unit. A significant diminution in mean nuclear size of the cell nucleus occurs in the case of increased cell densities of 215 and 277 cells/unit, corresponding to a cell culture of 2.5 × 10⁵ and 7.3 × 10⁵ cells ml. This diminution may be connected with the accumulation of Gl cell nuclei in cultures with contact-dependent growth inhibition.