Die Anaphylaxie

Doerr, Robert

doi:10.1007/978-3-662-26366-2

Cited by 13 publications

(4 citation statements)

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“…In the very early studies on sensitization it was re ported that the intravenous infusion of normal serum into sensitized animals protected the recipient from anaphylaxis when the homologous antigen was in jected [3]. We reported the presence of a serum factor in rats, humans and guinea pigs which blocked the PCA reaction of mouse IgE in rat skin [8].…”

Section: Introductionmentioning

confidence: 98%

Characterization of a Factor from Rat Serum with Blocking Activity in the Passive Cutaneous Anaphylactic Reaction

Theobald

König

1983

Int Arch Allergy Immunol

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A blocking factor (BF) was obtained from normal and rat reaginic serum which inhibited the passive cutaneous anaphylactic (PCA) reaction of mouse and rat IgE in rat skin. Several chromatographic procedures on DEAE- and CM-cellulose at various pH values were compared in an attempt to obtain BF with high activity. BF-containing fractions after DEAE chromatography at pH 5,4 revealed two components on SDS-polyacrylamide gel: one at 50,000–60,000 and the other at 65,000–70,000 daltons. The latter component was also obtained when normal or rat reaginic serum was adsorbed on Sepharose anti rat IgG. The eluate inhibited the PCA reaction and demonstrated two bands on SDS-polyacrylamide gel, one at 150,000 (IgG) and another at 65,000–70,000 daltons. Purified IgG and rat albumin did not inhibit the PCA reaction. Antisera against BF were obtained with the component at 65,000–70,000 daltons. Anti-BF revealed a specificity for two bands in the cathodal position when assayed against normal rat serum; one of which was IgG. Anti-BF recognized one component in the DEAE fraction when analyzed against the DEAE fraction which contained blocking activity. BF is immunologically distinct from IgG. After immunoadsorption of normal or rat reaginic serum on Sepharose anti-BF columns, an eluate was obtained which showed blocking activity in the PCA reaction and two components on SDS-polyacrylamide gel, one at 150,000 and the other at 65,000–70,000 daltons. Evaluation of blocking factor by isoelectric focussing obtained after various procedures revealed an isoelectric point of 4.7. BF shows dual functions: it inhibits and at high dilutions also enhances the PCA reaction. It is suggested that this factor has a modulatory role in the allergic disease process.

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Section: Introductionmentioning

confidence: 98%

Characterization of a Factor from Rat Serum with Blocking Activity in the Passive Cutaneous Anaphylactic Reaction

Theobald

König

1983

Int Arch Allergy Immunol

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“…In the very early reports on sensitization of tissue with 'reagins', it was noted that the intravenous infusion of normal serum into a sensitized animal protected the recipient from anaphylaxis when the appropriate an tigen was given [4]. In our report, experi ments are presented which show, that addi tion of normal serum to reaginic antibody blocks the PCA reaction.…”

Section: Introductionmentioning

confidence: 54%

On the Latency Period of Sensitization: Inhibition of Passive Cutaneous Anaphylaxis by a Factor in Normal Rat Serum

König¹,

Henn²

1979

Int Arch Allergy Immunol

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Addition of normal rat serum to mouse or rat IgE antibody blocks the passive cutaneous sensitization of rats with either IgE. The inhibition can be obtained with isologous and autologous rat serum. The blocking factor was purified and characterized as a molecule with a MW of 69 000 daltons, a sedimentation rate of 4.5 s and an isoelectric point of pH 4.7. The factor blocks the PCA reaction also at previously sensitized skin sites and seems to act on the secretory phase of mediator release.

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“…Die Grundvoraussetzung für eine derartige Deutung, Propor tionalität zwischen Antigendosis -Menge reagierender Antikörper -Symptomintensität, scheint genügend gesichert (19,20,(29)(30)(31)(32)(33).…”

Section: Anteil Einer Langsamen Spasmolyse Allerdings Nicht Abzugrenzunclassified

Versuche über den Anteil von Histamin am anaphylaktischen Asthma sowie Beobachtungen über Auslösung und Verhalten der Asthma-und Kreislaufsymptoine anaphylaktischer Schockfragmente des Meerschweinchens (Part 2 of 2)

Alberty¹

1959

Int Arch Allergy Immunol

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Die Anaphylaxie

Cited by 13 publications

References 0 publications

Characterization of a Factor from Rat Serum with Blocking Activity in the Passive Cutaneous Anaphylactic Reaction

Characterization of a Factor from Rat Serum with Blocking Activity in the Passive Cutaneous Anaphylactic Reaction

On the Latency Period of Sensitization: Inhibition of Passive Cutaneous Anaphylaxis by a Factor in Normal Rat Serum

Versuche über den Anteil von Histamin am anaphylaktischen Asthma sowie Beobachtungen über Auslösung und Verhalten der Asthma-und Kreislaufsymptoine anaphylaktischer Schockfragmente des Meerschweinchens (Part 2 of 2)

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