On the Latency Period of Sensitization: Inhibition of Passive Cutaneous Anaphylaxis by a Factor in Normal Rat Serum

König, Wolfgang; Henn, Horst-Wolfram

doi:10.1159/000232191

Cited by 13 publications

(19 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inhibition pro duced by purified IgE paralleled that pro duced by scrum IgE when the concentration of both was expressed as ng/ml IgE. We conclude that the inhibitory capacity of al lergic serum is dependent on its content of IgE and that non-IgE serum components have no effect on human mast cell sensitiza tion, again contrasting with rat mast cells [10]. Sensitization of human mast cells by antigen-specific IgE is dependent not only on the extracellular concentration of that species of IgE but is influenced significantly by the relative concentrations of other IgE species.…”

mentioning

confidence: 63%

Inhibition of Human Lung Mast Cell Sensitization by IgE in Allergic Sera

Coleman¹,

Godfrey²

1981

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Passive sensitization of mast cells in human lung tissue by antigen-specific IgE was inhibited by allergic sera containing IgE of other specificities. IgE-free sera and heat-treated sera failed to inhibit. Inhibition by purified myeloma IgE was quantitatively similar to that produced by serum IgE. It is concluded that IgE antibodies of different antigen specificity at concentrations found in allergic sera compete for cell surface receptors on human mast cells.

show abstract

mentioning

confidence: 63%

Inhibition of Human Lung Mast Cell Sensitization by IgE in Allergic Sera

Coleman¹,

Godfrey²

1981

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…We reported the presence of a serum factor in rats, humans and guinea pigs which blocked the PCA reaction of mouse IgE in rat skin [8].…”

Section: Introductionmentioning

confidence: 97%

Characterization of a Factor from Rat Serum with Blocking Activity in the Passive Cutaneous Anaphylactic Reaction

Theobald

König

1983

Int Arch Allergy Immunol

View full text Add to dashboard Cite

A blocking factor (BF) was obtained from normal and rat reaginic serum which inhibited the passive cutaneous anaphylactic (PCA) reaction of mouse and rat IgE in rat skin. Several chromatographic procedures on DEAE- and CM-cellulose at various pH values were compared in an attempt to obtain BF with high activity. BF-containing fractions after DEAE chromatography at pH 5,4 revealed two components on SDS-polyacrylamide gel: one at 50,000–60,000 and the other at 65,000–70,000 daltons. The latter component was also obtained when normal or rat reaginic serum was adsorbed on Sepharose anti rat IgG. The eluate inhibited the PCA reaction and demonstrated two bands on SDS-polyacrylamide gel, one at 150,000 (IgG) and another at 65,000–70,000 daltons. Purified IgG and rat albumin did not inhibit the PCA reaction. Antisera against BF were obtained with the component at 65,000–70,000 daltons. Anti-BF revealed a specificity for two bands in the cathodal position when assayed against normal rat serum; one of which was IgG. Anti-BF recognized one component in the DEAE fraction when analyzed against the DEAE fraction which contained blocking activity. BF is immunologically distinct from IgG. After immunoadsorption of normal or rat reaginic serum on Sepharose anti-BF columns, an eluate was obtained which showed blocking activity in the PCA reaction and two components on SDS-polyacrylamide gel, one at 150,000 and the other at 65,000–70,000 daltons. Evaluation of blocking factor by isoelectric focussing obtained after various procedures revealed an isoelectric point of 4.7. BF shows dual functions: it inhibits and at high dilutions also enhances the PCA reaction. It is suggested that this factor has a modulatory role in the allergic disease process.

show abstract

“…SDS gel electrophoresis was performed as de scribed by König and Henn [10]. Protein coupling to paper discs was carried out according to Cuatrecasas [7].…”

mentioning

confidence: 99%

“…Protein coupling to paper discs was carried out according to Cuatrecasas [7]. Histamine release in vitro from human basophilic leukocytes (LMBs) was measured as described by Kö nig and Henn [ 10].…”

mentioning

confidence: 99%

“…Since the existence of anti gen-specific circulating IgE does not necessarily prove its biological relevance, we analyzed the wheat flour fractions for histamine release from human LMBs in vitro. Basophils from an allergic baker were taken and purified as described by König and Henn [10]. Whole flour extract and the flour precipitates were analyzed in dilutions of 1:5, 1:50, 1:500 and 1:5,000.…”

mentioning

confidence: 99%

See 1 more Smart Citation