Characterization of a Factor from Rat Serum with Blocking Activity in the Passive Cutaneous Anaphylactic Reaction

Theobald, K.; König, Wolfgang

doi:10.1159/000233265

Cited by 8 publications

(18 citation statements)

References 5 publications

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“…In agreement with Theobald and König [14] our experiments showed that, whatever the procedure of purification used, the yield of the biological activity was low. The inhibitory activity was found only when the constant presence of a band of 76,000 daltons was always associated either to 2 bands (55,000 and 26,000 daltons in fraction C of P.50) or to a band corre sponding to the serum albumin (fractions SF5, SF6, SF7 of P.70).…”

Section: Discussionsupporting

confidence: 91%

“…About the purification of the BF, Theobald and König [14] showed that their BF found in normal rat and reaginic serum was characterized as a 70,000-dalton molecule that migrates or comigrates with the al bumin fraction. They have clearly shown that inhibi tion was neither due to albumin nor IgG-related.…”

Section: Discussionmentioning

confidence: 99%

“…König and Henn [13] and Theobald and König [14] characterized a blocking factor (BF) obtained from normal and rat reaginic serum which inhibited the PCA reaction of mouse and rat IgE in rat skin. They suggested that BF acts on the secretory phase of medi ator release and has a modulatory role in the allergic disease process.…”

Section: Introductionmentioning

confidence: 99%

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Induction of a Passive Cutaneous Anaphylaxis Inhibitory Factor in Mice

Mécheri¹,

Peltre²,

David³

1985

Int Arch Allergy Immunol

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The administration of a purified protein from Dactylis glomerata pollen, named Dactylis inhibitory protein (DIP), to Balb/C mice results in the production of a heat-resistant seric factor that blocks the passive cutaneous anaphylaxis (PCA) reaction in rat skin. This PCA inhibitory factor (PCA-IF) was purified following different purification procedures and analyzed in sodium dodecyl sulfate polyacrylamide gel. Amongst the array of bands detected it was possible to ascribe the inhibitory activity to a protein band of 76,000 daltons but only when it was associated either with another protein band of 69,000 daltons (obtained after a one-step purification procedure) or two other protein bands of 55,000 and 26,000 daltons (obtained after a three-step purification procedure). The inhibitory activity of the PCA-IF is present at a low level in normal Balb/C mice sera and is enhanced after DIP treatment of the mice.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Induction of a Passive Cutaneous Anaphylaxis Inhibitory Factor in Mice

Mécheri¹,

Peltre²,

David³

1985

Int Arch Allergy Immunol

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“…It has been established in the past that sera of var ious mammalian species can inhibit the P CA reaction and the histamine release from rat mast cells and hu man basophil leukocytes in vitro and in vivo [1][2][3][4], Recently, our studies were directed towards the isola tion o f this molecule from human serum. It appears that at least two factors can mediate this reaction.…”

Section: Discussionmentioning

confidence: 99%

“…During the last decade, several studies were re ported with regard to serum factors with modulatory activity for histamine release in vivo and in vitro [1][2][3][4], For rat serum the active molecule was identified as a protein o f approximately 70,000 daltons and an IEP at pH 4.8 [2]. In human serum using the same pu rification procedure a protein with very similar char acteristics was obtained.…”

Section: Introductionmentioning

confidence: 99%

Purification of Serum Proteins with Inhibitory Activity on the Histamine Release in vitro and/or in vivo

Theobald¹,

Groß-Weege²,

Keymling³

et al. 1987

Int Arch Allergy Immunol

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Recently, we described a factor in rat and human serum with inhibitory activity on the 2-hour passive cutaneous anaphylactic (PCA) reaction in rat skin. The Ca-ionophore-induced histamine release from rat peritoneal mast cells was inhibited as well. In human serum, inhibitory activity for the in vitro histamine release from rat mast cells was shown to be present within two serum fractions. One molecule was purified by 50–70% ammonium sulfate precipitation and subsequent DEAE chromatography at pH 5.4. The main component showed complete identity with transferrin. Commercially available transferrin was active in vitro as well. The dose-response plot revealed two inhibition maxima at transferrin concentrations of 0.05–5 ng/ml and at more than 1 mg/ml, respectively. Subsequently it was shown that the degree of iron saturation was critical for the decrease in histamine release. The mediator release in vivo (PCA) could not be inhibited by the iron transport protein. Inhibitory activity in vivo was however mediated by the second serum fraction, which was characterized by a molecular weight of approximately 150,000 daltons and an IEP within the alkaline range. These data suggest that exogenous factors may be potent modulators of inflammatory reactions.

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Interaction of monoclonal antibodies with the IgE-receptor on rat mast cells and rat basophilic leukemia (RBL) cells

et al. 1986

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The close relation between rat mast cells and rat basophilic leukemia (RBL) cells with regard to the presence of receptors for IgE and Fc gamma led us to generate monoclonal antibodies directed against cell surface antigens. Hybridomas were obtained by the fusion of NS1 mouse myeloma cells with murine spleen and lymph node cells. The culture supernatants were assayed by two ELISA techniques: a) for the production of mouse immunoglobulin in general and b) for antibodies directed against surface antigens of RBL cells. For this purpose RBL cells were attached to polyvinyl chloride microtitre plates. Eight hybrids produced antibodies directed against surface antigens on RBL cells. Hybrids were cloned and characterized with regard to their isotype and light chains. All eight clones secreted IgM with K light chains. Immunofluorescence studies performed with RBL cells revealed that all eight antibodies were able to show a specific fluorescence. Furthermore, four of these eight antibodies also showed a specific fluorescence with purified rat mast cells. These four antibodies were analyzed as to their ability of interacting with the IgE-receptor on RBL cells and purified rat mast cells. They reduced the binding rate of radiolabelled rat IgE to RBL and rat mast cells. A mutual inhibition of the passive cutaneous anaphylaxis (PCA) reaction in the rat by either mixing mouse reaginic serum directed against 2,4-dinitrophenol bovine serum albumin (DNP-BSA) or by mixing monoclonal mouse anti-DNP IgE with the monoclonal mouse anti-cell surface (rat basophilic leukemia, rat mast cell) IgM was determined.(ABSTRACT TRUNCATED AT 250 WORDS)

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Characterization of a Factor from Rat Serum with Blocking Activity in the Passive Cutaneous Anaphylactic Reaction

Cited by 8 publications

References 5 publications

Induction of a Passive Cutaneous Anaphylaxis Inhibitory Factor in Mice

Induction of a Passive Cutaneous Anaphylaxis Inhibitory Factor in Mice

Purification of Serum Proteins with Inhibitory Activity on the Histamine Release in vitro and/or in vivo

Interaction of monoclonal antibodies with the IgE-receptor on rat mast cells and rat basophilic leukemia (RBL) cells

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