Diamine oxidase (DAO) activity and intestinal mucosa integrity: influence of suture techniques

Abstract

After various kinds of intestinal mucosal injuries, whether by disease or by experiment, the diamine oxidase activity is reduced. Therefore, we studied the effect of surgical manipulations on the intestinal mucosa and diamine oxidase activity. The reaction of the gut on the insertion of sutures was a transient increase of the enzymic activity followed by reduction as soon as the mucosa started to gain weight. After a standardized pressure injury only a reduction of the diamine oxidase activity together with an enhancement of the mass of the intestinal wall was found. A hypothesis of a feed-back regulation of the diamine oxidase activity connected with mucosal proliferation is proposed.

Paradigms for Mechanical Signal Transduction in the Intestinal Epithelium

“…In the small bowel, a standardized surgical pressure decreases diamine oxidase expression (a brush border enzyme) and increases mucosal mass [60]. Mucosal atrophy in defunctionalized Thery-Vella loops is reversed by luminal water [61], suggesting that mechanical distention supports the mucosa.…”

Significance of the Intestinal Gradient of Diamine Oxidase Activity

“…Such factors include age, vitamin deficiency, endocrine function, pregnancy, diet, malignancy and medications [3,[19][20][21][22], The extent of intes tinal disease and previous intestinal resec tion are obviously important. Whether loca tion of intestinal disease is an important determinant of intestinal and serum DAO activity is another consideration.…”

16. Advocatus diaboli-Kommentar

“…Der Berstungsdruck war nach Staplerapplikation zwar h6her, ebenso wie die Kollagensynthese [18,23], jedoch bei gr66erem Mucosatrauma [15,18]. Der Berstungsdruck war nach Staplerapplikation zwar h6her, ebenso wie die Kollagensynthese [18,23], jedoch bei gr66erem Mucosatrauma [15,18].…”