Dialysis culture of microorganisms: design, theory, and results.

Dialysis Culture for the Production of Extracellular Protein A from Staphylococcus aureus A676

“…Since the yields of staphylococci were comparatively low in the TSB, it was of interest to test if dialysis cultivation could give increased bacterial concentrations and higher yields of protein A in this medium. Dialysis cultivation has been shown to facilitate the production of very concentrated cultures of micro-organisms (Schultz & Gerhardt 1969). In most studies this has been ascribed to the provision of nutrients from the reservoir medium to the growing culture.…”

Manufacture of Starters: Production of Frozen Concentrated Cheese Starters by Diffusion Culture

“…The present system of diffusion culture as a process for production of concentrated cheese starter was based on data obtained from earlier prototypes. The first experimental systems were based on data given by Schultz & Gerhardt (1969) where each ml of fermenter culture was in contact with approximately 1 cm2 of membrane area and the fermenter/reservoir working volume ratio was 1 : 10 i.e. with a fermenter volume of 1 1 the membrane area would be 1000 cm2 and reservoir volume 10 1.…”

“…Gerhardt and his co-workers were successful however, in producing concentrated cultures of organisms other than lactic streptococci. A review by Schultz & Gerhardt (1969) gives a comprehensive theoretical treatment of the subject and some basic parameters for equipment design.…”

Effects of Degradative Microbial Enzymes on the Growth and Structure of Young Carrot Roots

“…We thank Dr. PHAFF for a culture of the organism. In addition to enzyme purification by adsorption to its substrate and subsequent elution as devised by PHAFF, we obtained a good yield by growing the organism inside a dialysis bag which was suspended in Yeast Nitrogen Base medium (Difco Lab., Detroit, Midiigan) containing 3% glucose (SCHULTZ and GERHARDT 1969). The medium was stirred continuously.…”