Dialysis Culture for the Production of Extracellular Protein A from Staphylococcus aureus A676

Abstract

Different nutrient media have been tested for the production of extracellular protein A from Staphylococcus aureus A676. High yields were obtained in trypticase soy broth with glucose as the main energy source. In aerated fermenter cultures the amount of protein A obtained was 0·2–0·3 gl−1, as compared to reported yields of 0·03 gl−1. Yields were further increased in dialysis cultures, where protein A concentrations of 2 gl−1. were achieved.

Specific attachment of Staphylococcus aureus to immobilized fibronectin

“…Cells from these strains were defined concerning their interactions with soluble fibronectin (40) and IgG (41a). Bacteria were kept on deep agar at -70°C, cultured on blood agar, and subsequently grown in tryptic soy broth (16). The tryptic soy broth medium was supplemented (4 ml/liter) with a solution containing calcium pantothenate (2 mg/ml), thiamine (1 mg/ml), D-biotin (0.01 mg/ml), and nicotinamide (1.2 mg/ml), all dissolved in PBS-D (0.14 M NaCl, 2.7 mM KCl, 1 mM CaCl2, 0.5 mM MgCl2, 8 mM Na2HPO4, 1.5 mM NaH2PO4, pH 7.4).…”

Dialysis culture for the production of Pseudomonas saccharophila α-amylase

“…However, toxicity of the resin itself may be a problem (Abbot and Gerhardt 1970). Application of dialysis culture in the production of diffusible and cell-associated products has been reported (Abbot and Gerhardt 1970;Landwall 1978). The method is particularly useful for the production of high molecular weight products secreted by growing cells as these tend to concentrate in the small volume of the culture vessel.…”