Specific attachment of Staphylococcus aureus to immobilized fibronectin

Abstract

Staphylococcus aureus cells have been shown to possess surface-associated proteins with affinity for soluble fibronectin. We have investigated the ability of these surface proteins to mediate attachment to immobilized fibronectin and collagen. Attachment was quantified by determination of bacterial ATP in a bioluminescence assay. The ability to attach to fibronectinor collagen-coated plastic surfaces was investigated for four S. aureus strains: Cowan 1, Newman, SA113(83A), and Wood 46. Cells from the different strains varied in their attachment properties, but all cells except those of strain Wood 46 attached readily to substrates coated with fibronectin. Only cells from strain Cowan 1 attached reproducibly to collagen-coated substrates in the absence of fibronectin. The attachment of cells from strain SA113(83A) to fibronectin-coated surfaces was shown to be dependent on time, fibronectin concentration, and bacterial growth phase. Soluble fibronectin or NH2-terminal fibronectin fragment (Mr, 29,000) disturbed the attachment to surfaces coated with fibronectin bound to denatured collagen type I. The attachment process to such substrates was also effectively inhibited by preincubating the substrate with fibronectin-binding proteins isolated from S. aureus Newman and SA113 (83A) and purified with affinity chromatography.

Agr‐independent regulation of fibronectin‐binding protein(s) by the regulatory locus sar in Staphylococcus aureus

“…1) than strain ISP479C, another subculture of 8325-4. Whereas our binding data are consistent with some previous reports (Lindberg et al, 1990;Maxe et al, 1986) showing that strain Newman can easily bind both soluble and solid phase fibronectin, others found this strain to be deficient in binding to fibronectin-coated polymethyl methacrylate coverslips. Because the source of the parental strain Newman used in this work and in Vaudaux et al (1995) is apparently the same, the discrepancy may be due to either spontaneous mutations during subculturing in the different laboratories or differences in the performance of the binding assays.…”

Specific binding of bone sialoprotein to Staphylococcus aureus isolated from patients with osteomyelitis

“…The binding between staphylococcal cells and fibronectin has been extensively characterized. Thus, a cell surface fibronectin-binding protein(s) has been isolated [8,281. These fibronectin-binding proteins are not sensitive to heat nor to treatment with sodium dodecyl sulfate [I, 3,81.…”

Isolation and Characterization of Teichoic Acid‐Like Substance as an Adhesin of Staphylococcus aureus to HeLa Cells

“…Bacterial adherence to a host tissue is generally the initial event of any bacterial disease and it involves specific binding between molecules on the surface of both the host and bacterial cells (5). Staphylococcus aureus has three known adhesins, the laminin receptor (21), fibronectin receptor (13,14,22,24,25), and collagen receptor (17,26), and some putative adhesins, including teichoic acid (2,3,(6)(7)(8), lipoteichoic acid (9), protein A (11), etc. S. aureus may have other adhesins because host cells have many kinds of molecules other than the known extracellular matrices on the surface.…”