Diagnóstico laboratorial. Avaliação de métodos de diagnóstico das principais doenças infecciosas, parasitárias e auto-imunes. Correlação clínico-laboratorial

Ferreira, Antônio Walter; Ávila, Sandra do Lago Moraes de

doi:10.1590/s0036-46651996000400017

Cited by 34 publications

(37 citation statements)

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“…Diagnostic performance of ELISA using different peptides. The diagnostic parameters were defined in terms of sensitivity, specificity and efficiency, according to FERREIRA & AVILA (2001) 8 . The sensitivity was determined independently for groups I9 (25), II (25) and III(100): (ELISA positive)/(Positive by conventional reference data).…”

Section: Elisa With Individual Peptides (Sag-1 Gra-1 and Gra-7)mentioning

confidence: 99%

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

Araújo

Ferreira

2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe main serological marker for the diagnosis of recent toxoplasmosis is the specific IgM antibody, along with IgG antibodies of low avidity. However, in some patients these antibodies may persist long after the acute/recent phase, contributing to misdiagnosis in suspected cases of toxoplasmosis. In the present study, the diagnostic efficiency of ELISA was evaluated, with the use of peptides derived from T. gondii ESA antigens, named SAG-1, GRA-1 and GRA-7. In the assay referred to, we studied each of these peptides individually, as well as in four different combinations, as Multiple Antigen Peptides (MAP), aiming to establish a reliable profile for the acute/recent toxoplasmosis with only one patient serum sample. The diagnostic performance of the assay using MAP1, with the combination of SAG-1, GRA-1 and GRA-7 peptides, demonstrated better discrimination of the acute/recent phase from non acute/ recent phase of toxoplasmosis. Our results show that IgM antibodies to MAP1 may be useful as a serological marker, enhancing the diagnostic efficiency of the assay for acute/recent phase of toxoplasmosis.

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Section: Elisa With Individual Peptides (Sag-1 Gra-1 and Gra-7)mentioning

confidence: 99%

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

Araújo

Ferreira

2010

Rev. Inst. Med. trop. S. Paulo

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“…These yeasts are widely distributed in the environment and frequently colonize skin and mucous membranes such as oral cavity and genital and gastrointestinal tracts of mammalians (9,12,20,21,32). In dogs, yeasts belonging to Candida genus prefer constantly humid areas, which favor tissue maceration, as occurs in mucous membranes, mucocutaneous junctions, intertriginous areas, nail substructure *Corresponding Author.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Candida spp from vaginal microbiota of healthy canine females during estrous cycle

Cleff¹,

Lima²,

Faria³

et al. 2005

Braz. J. Microbiol.

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Yeasts are commensal organisms found in the skin, genital and gastrointestinal tracts, and other mucosa in mammalians. Candida spp has been frequently isolated from domestic and sylvan animals. Yeast isolation from vaginal mucus is frequent in women, especially of Candida genus. In canine females, however, studies about vaginal fungal microbiota are rare and the connection with estrous cycle is unknown. The purpose of this study was to isolate yeast 5 of Candida genus from healthy canine females and identify the isolated species, establishing their connection with estrous cycle phases. Two hundred twenty-four samples taken from vaginal mucus of 14 canine females were analyzed in this study. Candida spp was observed in 83 (37%) from these samples; nine samples were taken in proestrus, 14 in oestrus, 31 in dioestrus, 24 in anoestrus and five samples were obtained during pregnancy. Candida parapsilosis the most frequently isolated specie (21.7%), followed by C. guillermondii (8.4%), C. kefir (6%) and C. albicans (4.8%). We concluded that Candida spp is a commion component of healthy canine females' vaginal microbiota, and isolation varies according to the estrous cycle phase.

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“…The occurrence of Malassezia is verified in both sex (6,7,33) but the prevalence of pityriasis versicolor is observed more frequently in male (21,27), however other authors indicated that the incidence is higher in women (31). The role of sex in propensity to development of pityriasis versicolor is still unclear (37).…”

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confidence: 99%

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

Neves¹,

Magalhães²,

Silva³

et al. 2005

Braz. J. Microbiol.

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This study investigated the ocurrence of Malassezia species in clinically healthy students and with macules with a slight fawn discoloration and characterized the isolates as to the pathogenicity factors such as growth at 37ºC, lipase, phospholipase and protease detection. Clinical samples were collected from different body sites of one hundred students of different ages and both sexes. The samples, obtained by scrapping the skin surface and the scalp, were treated with potassa and cultured. Cultures were obtained in Petri dishes containing Sabouraud agar medium added of olive oil, incubated at room temperature and at 37ºC. Culture identifications were based in their morphological and physiological properties. Lipase, phospholipase and protease detection was performed in specific media on Petri dishes for formation of a zone. Globose, spherical yeast cells and hypha were investigated by direct microscopy of clinical materials. Malassezia furfur was detected in seven samples and M. sympodialis in four. All Malassezia cultures presented lipase activity, but none was phospholipase positive. Protease activity was observed in two M. furfur and two M. simpodialis isolates. Key words: Malassezia furfur, M. simpodialis, pathogenicityMalassezia is a lipophilic yeast frequently found in normal human skin. Current evidence indicates a high rate of skin colonization in healthy adults, in contrast with the low rate of colonization in prepubertal children (20).Gueho et al. (11) (14,17,35,38). With the exception of M. pachidermatis, the remaining eight species have an absolute in vitro requirement for suplementation of long-chain fatty acids. Based on studies conducted within the last 5 years, it appears that the most frequently organism associated with pityriasis versicolor may not be M. furfur and possible candidates include M. globosa (2,27) and M. sympodialis (15). The genus Malassezia was recently revised, but the clinical significance of each of these species is not clearly understood (15).Pityriasis versicolor (tinea versicolor) is a superficial fungal infection of the skin, appearing most commonly on the upper trunk, as well as on the upper arms, neck and face. As suggested by its name, pityriasis versicolor may be manifested in different colours, ranging from pink or to dark brown or even black. The lesions have a characteristic flaking appearance, although in larger lesions this can be evident only at the border of the macule, and can be hypopigmented or hyperpigmented. Generally, the lesions take the form of round or oval macules or papules, although in advanced cases the lesions may become confluent (13,21,33).

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Diagnóstico laboratorial. Avaliação de métodos de diagnóstico das principais doenças infecciosas, parasitárias e auto-imunes. Correlação clínico-laboratorial

Cited by 34 publications

References 0 publications

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

High diagnostic efficiency of IgM-ELISA with the use of multiple antigen peptides (MAP1) from T. gondii ESA (SAG-1, GRA-1 and GRA-7), in acute toxoplasmosis

Isolation of Candida spp from vaginal microbiota of healthy canine females during estrous cycle

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

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