Isolation of Candida spp from vaginal microbiota of healthy canine females during estrous cycle

Abstract

Yeasts are commensal organisms found in the skin, genital and gastrointestinal tracts, and other mucosa in mammalians. Candida spp has been frequently isolated from domestic and sylvan animals. Yeast isolation from vaginal mucus is frequent in women, especially of Candida genus. In canine females, however, studies about vaginal fungal microbiota are rare and the connection with estrous cycle is unknown. The purpose of this study was to isolate yeast 5 of Candida genus from healthy canine females and identify the isolated species, establishing their connection with estrous cycle phases. Two hundred twenty-four samples taken from vaginal mucus of 14 canine females were analyzed in this study. Candida spp was observed in 83 (37%) from these samples; nine samples were taken in proestrus, 14 in oestrus, 31 in dioestrus, 24 in anoestrus and five samples were obtained during pregnancy. Candida parapsilosis the most frequently isolated specie (21.7%), followed by C. guillermondii (8.4%), C. kefir (6%) and C. albicans (4.8%). We concluded that Candida spp is a commion component of healthy canine females' vaginal microbiota, and isolation varies according to the estrous cycle phase.

Presença De Fungos Com Potencial Patogênico Em Instrumentos De Tosa

“…Em veterinária, as espécies C. catenulata, C. guilliermondii e C. famata foram isoladas de vacas com mastite no Brasil, Japão, Polônia e Itália (Moretti et al, 1998;Spanamberg et al, 2008). Em cães hígidos, C. parapsilosis foi isolada em amostras de mucosas vaginal, oral, perianal e prepucial (Cleff et al, 2005;Brito et al, 2009). A candidose em animais está relacionada à C. albicans, com relatos em cães, gatos e macacoprego (Ferreiro et al, 2002;Cleff et al, 2008;Ong et al, 2010).…”

MalasseziaversusCandidain Healthy Dogs

“…B r i t o et al [5] and C l e f f et al [11] stated, that Candida genus is considered to be part of the microbiota of dogs. We did not identify Candida yeasts on the skin of our examined dogs.…”

“…Generally, lipophilic M. pachydermatis is the main colonizing yeast in healthy and also in diseased dogs [31]. Only a few times lipid-dependent Malassezia yeasts (M. furfur, M. nana and M. obtusa) were identified [11,12,14,15,36].…”

Clinical and mycological analysis of dog's oral cavity

“…However, Pinter and Noble (1998) mentioned a relation among oral diseases in dogs and this yeast in a stomatitis, pharyngitis and tonsillitis case. M. pachydermatis is the most widely studied yeast species in animals, and it is thought to make part of the microbiota of various anatomical sites, mainly the external ear and skin (Nobre et al , 1998; Bond et al , 2000; Cleff et al , 2005). However, Nobre et al (1998) reported that at an imbalance condition between host and microorganisms occurs, this yeast can turns pathogenic due to a cell number increase referred to a predisposing factor.…”