Diagnostic Value of the Intradermal Chancroidal Test

Greenblatt, Robert B.; Sanderson, Everett S.

doi:10.1001/archderm.1937.01480030013002

Cited by 9 publications

(3 citation statements)

References 3 publications

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“…Saelhof (116) reported isolation rates in 1924 of 65% and discussed the effects of media, temperature, and moisture on H. ducreyi viability. Because of the continuing difficulty in isolation and subsequent identification of H. ducreyi, extensive effort was directed towards the development of immu-nological diagnostic methods and the production of skin test antigens (40,55,129). Nevertheless, in 1935 the U.S. Public Health Service, like the British Medical Research Committee two decades earlier, found that chancroid "is a local disease of the external generative organs in which a sore develops.…”

Section: Later Descriptions Of the Organismmentioning

confidence: 99%

Biology of Haemophilus ducreyi.

Albritton¹

1989

Microbiological Reviews

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Section: Later Descriptions Of the Organismmentioning

confidence: 99%

Biology of Haemophilus ducreyi.

Albritton¹

1989

Microbiological Reviews

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“…J 1. 32 The complement fixation test gives falsepositive tests due to apparent cross reactivity between Hemophilus ducreyi and some other bacteria.a. 34 Culture is difficult.…”

Section: Diagnosismentioning

confidence: 99%

Chancroid: Alias the Soft Chancre

Gaisin

Heaton

1975

Int J Dermatology

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“…Several diagnostic criteria have been proposed in the past, but most of these have a low accuracy or yield low recovery rates (1,2,6,8). As a consequence, the diagnosis of chancroid is often based on the exclusion of other sexually transmitted diseases causing genital ulcers and on the clinical aspects of the lesions (5,9).…”

mentioning

confidence: 99%

Susceptibility of 40 Haemophilus ducreyi strains to 34 antimicrobial products

Slootmans¹,

Berghe²,

Dyck³

et al. 1983

Antimicrob Agents Chemother

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A study was performed to examine compounds that might improve the selectivity of the primary isolation medium for Haemophilus ducreyi. The susceptibility of 40 H. ducreyi strains to 34 antimicrobial agents, including 10 antibiotics, 3 quaternary ammonium compounds, 3 phenolic derivatives, 3 acridines, and 15 heavy metal compounds, was investigated by using an agar plate dilution technique. Results were compared with the susceptibilities of other gramnegative rods which may be contaminants on isolation media. The minimal inhibitory concentration range for colistin (16 to 128 p.g/ml) indicated that this antibiotic might be of use as a selective agent. H. ducreyi was susceptible to spectinomycin (minimal inhibitory concentration range, 16 to 64 t.g/ml), thiamphenicol (0.12 to 1 pug/ml), chloramphenicol (0.12 to 0.5 ig/ml), and streptomycin (4 to 32 ,g/ml) and moderately susceptible to kanamycin (2 to 8 ,ug/ml). For the heavy metal compounds, a high susceptibility was seen with copper(II) chloride (2 to 8 p,g/ml, corresponding to a concentration of 0.75 to 3 ,ug of Cu2+ ions per ml), sodium selenite (1 to 4 ,ug/ml, or 0.45 to 1.83 p.g of Se-ions per ml), and phenylmercury acetate (0.12 to 0.5 ,ug/ml). The minimal inhibitory concentrations of quaternary ammonium compounds, acridines, and phenolic derivatives were between 1 and 32 pg/ml, 8 and 32 ,ug/ml, and 8 and 250 ,ug/ml, respectively.The isolation of Haemophilus ducreyi from clinical material is often difficult because of a lack of sensitivity and specificity of the primary isolation medium. Several diagnostic criteria have been proposed in the past, but most of these have a low accuracy or yield low recovery rates (1,2,6,8). As a consequence, the diagnosis of chancroid is often based on the exclusion of other sexually transmitted diseases causing genital ulcers and on the clinical aspects of the lesions (5, 9). The isolation medium developed by Hammond et al. (7) and its subsequent modifications (3, 4, 10) contain vancomycin. While effectively inhibiting the gram-positive flora, this medium does not inhibit gram-negative rods which often contaminate isolation plates, particularly when vaginal or cervical specimens are cultured.In this study, the activity of 34 antimicrobial agents, including 10 antibiotics, 3 quaternary ammonium and 15 heavy metal compounds, 3 acridines, and 3 phenolic derivatives was evaluated against 40 strains of H. ducreyi. Eighteen clinical isolates ofEnterobacteriaceae were tested in the same way to define the relative susceptibilities to the same agent. The aim of the study was to select a compound that would enhance the selectivity of the isolation medium. MATERIALS AND METHODS Strains

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Diagnostic Value of the Intradermal Chancroidal Test

Cited by 9 publications

References 3 publications

Biology of Haemophilus ducreyi.

Biology of Haemophilus ducreyi.

Chancroid: Alias the Soft Chancre

Susceptibility of 40 Haemophilus ducreyi strains to 34 antimicrobial products

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