Diagnostic utility of various techniques used in body fluid cytology

Joshi, Dr.Archana; Mahajan,, Dr.Nidhi; Karmarkar, Dr.P.J.; Mahore, Dr Sadhana

doi:10.9790/0853-13151318

Cited by 23 publications

(39 citation statements)

References 12 publications

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“…Thapar et al [14] recorded increased cellularity in the cell block sections that had better morphological and architectural preservation; this could be of great help in achieving the correct diagnosis of the primary site. Grandhi et al [15] and Joshi et al [16] recorded similar findings with increased cellularity in the cell block sections.…”

Section: Discussionmentioning

confidence: 61%

DNA Ploidy Analysis and Cell Block Immunohistochemistry in the Diagnosis of Malignant Effusions

et al. 2019

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Background: Effusion cytology is a major diagnostic tool in medicine and has both therapeutic and prognostic implications. One of the dilemmas encountered is the differentiation between atypical cells and reactive mesothelial cells. The use of ancillary tools can reduce this grey zone and help to achieve a definitive diagnosis. Objectives: The main objective of this study was to evaluate the role of flow cytometry (FCM) and cell block with immunohistochemistry (IHC), along with the clinicoradiological investigations, to achieve a final diagnosis in effusion cytology to the maximum extent possible. Method: A prospective study was conducted. Effusion fluids, showing adequate amount and cellularity, were processed for conventional cytology, ploidy analysis by FCM, and cell block analysis, followed by IHC wherever required. Conventional cytological analysis was done by 2 independent pathologists, to look for interobserver variation, if any. The final result was achieved on the basis of integration of the results of the aforementioned studies, cytological details, clinicoradiological information, tissue biopsy findings, and follow-up. Result: A total of 90 samples were analyzed. On cytological examination, observer I categorized 60% samples as benign and 18.8% (n = 17) as malignant versus 58% categorized as benign and 23.3% (n = 21) as malignant by observer II. Observer I reported 19 (21.1%) equivocal cases and observer II reported 16 (17.7%). When both pathologists were considered together, the number of equivocal cases increased to 20. Sensitivity and specificity of FCM were 96.67 and 100%, respectively, and 100% for the cell block. On combining all techniques, the equivocal cases were resolved and a total of 33 cases were reported as malignant. However, 3 cases could still not be categorized and were labeled inconclusive. Conclusion: Conventional cytology combined with cell block IHC and FCM has the potential to minimize the requirement of tissue biopsy for confirmation. If the first sample is used judiciously for all the techniques, this may reduce the requirement for a second sample and possibly also the time required for a definite diagnosis and the initiation of therapy.

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Section: Discussionmentioning

confidence: 61%

DNA Ploidy Analysis and Cell Block Immunohistochemistry in the Diagnosis of Malignant Effusions

et al. 2019

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“…al. [15] The most common effusion in the present study is peritoneal (42.67%) followed by pleural, csf and pericardial effusion. The same findings were found in the study by Archana et.…”

Section: Discussionmentioning

confidence: 83%

“…al. [15] which showed peritoneal fluid to be maximum amongst the samples studied and number of male patients were higher as compared to female. In our study a significant difference between the results obtained by conventional smears as compared to cytocentrifuge which is in accordance with the study of Archana et.…”

Section: Discussionmentioning

confidence: 99%

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Cytological Diagnosis of Serous Effusions by Using Comparative Approach of Routine Staining and Cytospin Technique

S¹,

Awasthi²,

Dutta³

2017

Ann. Int. Med. Den. Res.

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Background: Cytological assessment of serous fluids is important not only for diagnosis of malignancies but may also give information of various inflammatory conditions like parasitic infestations, infection with bacteria, fungi or viruses and some immunological conditions. It helps in staging, prognosis and management of patients of malignancies. This study undertakes the assessment of the utility of cytospin method in increasing the sensitivity of cytodiagnosis of serous effusions in comparison to conventional staining method & bear its impact mainly in early and correct diagnosis of malignancies and hence patients management and prognosis. Aim: To study different body fluids for the presence or absence of local or systemic pathology of neoplastic, inflammatory, infective and immune mediated lesions, to evaluate the diagnostic efficacy and sensitivity of comparative approach of routine conventional smears and cytospin techniques. Methods: One hundred and fifty serous fluids were received for diagnostic evaluation. Along with conventional smear, fluids were cytocentrifuged. Smears obtained by each of techniques were scored for same four parameters. Statistical analysis with Wilcoxon rank sum test was performed and also Index of Qualitative Variation (IQV) was calculated to compare the results obtained by each of the above two methods. Results: Cellularity and additional yield for malignancy was obtained more accurately by Cytospin method than conventional method. Conclusion: The cytospin method provide high cellularity, better architectural patterns, morphological features and an additional yield of malignant cells, and thereby, increases the sensitivity of the cytodiagnosis when compared to conventional smear method.

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“…Similar findings were observed by various studies. [4][5][6] In our study peritoneal fluid was the commonest 92/185 (49.7%). Out of 185 cases, 175 were non neoplastic and 10 were neoplastic.…”

Section: Discussionmentioning

confidence: 99%

Exfoliative cytology of body fluids: a study from provincial hospital of Jammu region, India

Gupta¹,

Dewan

Raina³

et al. 2016

Int J Res Med Sci

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Diagnostic utility of various techniques used in body fluid cytology

Cited by 23 publications

References 12 publications

DNA Ploidy Analysis and Cell Block Immunohistochemistry in the Diagnosis of Malignant Effusions

DNA Ploidy Analysis and Cell Block Immunohistochemistry in the Diagnosis of Malignant Effusions

Cytological Diagnosis of Serous Effusions by Using Comparative Approach of Routine Staining and Cytospin Technique

Exfoliative cytology of body fluids: a study from provincial hospital of Jammu region, India

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