Diagnostic utility of p63/p40 in the histologic differentiation of salivary gland tumors: A systematic review

Sivakumar, N.; Narwal, Anjali; Pandiar, Deepak; Devi, Anju; Bansal, Deepty; Kamboj, Mala

doi:10.1016/j.oooo.2021.07.010

Cited by 13 publications

(12 citation statements)

References 32 publications

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“…p63 regulated via TGF-β/JNK signaling, is a diagnostic marker of salivary gland neoplasia [ 13 , 17 ]. To investigate the roles of p63 expression in human salivary gland duct adenocarcinoma, human salivary gland duct adenocarcinoma cell line A253 was transfected with siRNA-p63 in the presence of a TGF-β receptor type 1 inhibitor (EW-7197) and JNK inhibitor (SP600125), before GeneChip analysis was performed on the cells.…”

Section: Resultsmentioning

confidence: 99%

“…P63/p40 (ΔNp63) is used for diagnostic utility in the histologic differentiation of salivary gland tumors [ 13 ]. We investigated the expression and localization of p63, CGN, ZO-3, and OLFML3 in human salivary duct adenocarcinoma, using paraffin-embedded sections of salivary duct adenocarcinoma tissues.…”

Section: Resultsmentioning

confidence: 99%

“…As the data reported here, p63 plays a crucial role in salivary adenocarcinoma in vitro, whereas p63/p40 (ΔNp63) is used for diagnostic utility in the histologic differentiation of salivary gland tumors [ 13 ]. In the present study, in salivary gland duct adenocarcinoma gland-like structures, the expression of CGN and ZO-3, but not p63, was detected, and OLFML3 was decreased in the cancer cells compared to normal ones, while in the solid type of salivary gland duct adenocarcinoma, CGN, ZO-3, and OLFML3 were not detected, while p63 was detected in some cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…The human TP63 gene is aligned with histone H3 acetylated at lysine 27 (H3K27Ac) [ 12 ]. p63/p40 (ΔNp63) is used for diagnostic utility in the histologic differentiation of salivary gland tumors [ 13 ]. However, the roles and regulation of p63 are unknown in salivary gland tumors.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of HDAC and Signal Transduction Pathways Induces Tight Junctions and Promotes Differentiation in p63-Positive Salivary Duct Adenocarcinoma

Nakano

Ohwada

Shindo

et al. 2022

Cancers

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Background: The p53 family p63 is essential for the proliferation and differentiation of various epithelial basal cells. It is overexpressed in several cancers, including salivary gland neoplasia. Histone deacetylases (HDACs) are thought to play a crucial role in carcinogenesis, and HDAC inhibitors downregulate p63 expression in cancers. Methods: In the present study, to investigate the roles and regulation of p63 in salivary duct adenocarcinoma (SDC), human SDC cell line A253 was transfected with siRNA-p63 or treated with the HDAC inhibitors trichostatin A (TSA) and quisinostat (JNJ-26481585). Results: In a DNA array, the knockdown of p63 markedly induced mRNAs of the tight junction (TJ) proteins cingulin (CGN) and zonula occuludin-3 (ZO-3). The knockdown of p63 resulted in the recruitment of the TJ proteins, the angulin-1/lipolysis-stimulated lipoprotein receptor (LSR), occludin (OCLN), CGN, and ZO-3 at the membranes, preventing cell proliferation, and leading to increased cell metabolism. Treatment with HDAC inhibitors downregulated the expression of p63, induced TJ structures, recruited the TJ proteins, increased the epithelial barrier function, and prevented cell proliferation and migration. Conclusions: p63 is not only a diagnostic marker of salivary gland neoplasia, but it also promotes the malignancy. Inhibition of HDAC and signal transduction pathways is, therefore, useful in therapy for p63-positive SDC cells.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of HDAC and Signal Transduction Pathways Induces Tight Junctions and Promotes Differentiation in p63-Positive Salivary Duct Adenocarcinoma

Nakano

Ohwada

Shindo

et al. 2022

Cancers

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“…As a result, immunohistochemistry for squamous immunohistochemical markers like p40, p63, and CK5/6 was often relied on to confirm definitional squamous differentiation in MEC. [7][8][9][10][11][12] The vast majority of MEC has also been found to harbour CRCT1::MAML2 or CRCT3:: MAML2 fusions, which are regarded as specific to this entity in the salivary glands and are desirable criteria in the WHO Classification. 6,13,14 Not only can MAML2 testing confirm a challenging diagnosis, but identification of MAML2 fusions as a genetic gold standard has allowed for recognition of multiple variant forms of MEC, including oncocytic, ciliated, sclerosing, Warthin-like, and mucoacinar types.…”

Section: Introductionmentioning

confidence: 99%

Mucoepidermoid carcinoma may be devoid of squamoid cells by immunohistochemistry: expanding the histologic and immunohistochemical spectrum of MAML2‐ rearranged salivary gland tumours

et al. 2022

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Mucoepidermoid carcinoma (MEC) is historically defined by a mix of squamoid, intermediate, and mucous cells, but we have recently encountered several cases lacking immunoreactivity for squamous markers p40, p63, and CK5/6 despite MAML2 fusions. This study will characterise these unique tumours. Ten MEC were collected arising from the parotid gland (n = 4), submandibular gland (n = 2), nasopharynx (n = 1), base of tongue (n = 1), bronchus (n = 1), and trachea (n = 1). Six tumours were low‐grade, two intermediate‐grade, one high‐grade, and one demonstrated low‐grade areas with high‐grade transformation. Four cases were oncocytic, four had clear‐cell features, two had spindle cell features, and one high‐grade MEC had prominent solid, cord‐like, and micropapillary features. The tumours were negative for p40 (10/10), p63 (10/10), and CK5/6 (9/9). Targeted RNA sequencing demonstrated CRTC1::MAML2 in five cases, CRTC3::MAML2 in two, and a novel MAML2::CEP126 in the unusual high‐grade case. In two cases with insufficient RNA, MAML2 fluorescence in situ hybridisation (FISH) showed rearrangement. Genetically‐confirmed MEC may lack overt squamous differentiation by histology and immunohistochemistry. While most cases harboured canonical fusions and fit within the spectra of MEC variants with oncocytic, clear cell, and/or spindle cell features, one had a novel MAML2::CEP126 fusion and unusual morphology. In MEC without squamoid cells, the use of immunohistochemistry may hinder, rather than aid, the correct diagnosis. In such cases, MAML2 analysis is most useful. The historical definition of MEC as a carcinoma with squamoid, intermediate and mucous cells should be revisited.

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Diagnostic Utility of Expression Pattern of S100/Mammaglobin/SOX10/DOG 1 Immunohistochemistry in Differentiation of Secretory and Acinic Cell Carcinoma: A Systematic Review and Meta-Analysis

Sharma

Kamboj

Narwal

et al. 2023

Indian J Otolaryngol Head Neck Surg

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Diagnostic utility of p63/p40 in the histologic differentiation of salivary gland tumors: A systematic review

Cited by 13 publications

References 32 publications

Inhibition of HDAC and Signal Transduction Pathways Induces Tight Junctions and Promotes Differentiation in p63-Positive Salivary Duct Adenocarcinoma

Inhibition of HDAC and Signal Transduction Pathways Induces Tight Junctions and Promotes Differentiation in p63-Positive Salivary Duct Adenocarcinoma

Mucoepidermoid carcinoma may be devoid of squamoid cells by immunohistochemistry: expanding the histologic and immunohistochemical spectrum of MAML2‐ rearranged salivary gland tumours

Diagnostic Utility of Expression Pattern of S100/Mammaglobin/SOX10/DOG 1 Immunohistochemistry in Differentiation of Secretory and Acinic Cell Carcinoma: A Systematic Review and Meta-Analysis

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