Diagnostic markers selected by immunoproteomics and phage display applied for the serodiagnosis of canine leishmaniosis

Machado, Juliana Martins; Costa, Lourena E.; Dias, Daniel Lucas Santos; Ribeiro, Patrícia A.F.; Martins, Vívian T.; Lage, Daniela P.; Carvalho, Gerusa B.; Franklin, Michelle L.; Tavares, Grasiele S.V.; Oliveira‐da‐Silva, João A.; Machado, Amanda S.; Ramos, Luana S.; Nogueira, Lais Moreira; Mariano, Reysla Maria da Silveira; Moura, H.; Silva, Eduardo Sérgio da; Teixeira-Neto, Rafael Gonçalves; Campos-da-Paz, Mariana; Galdino, Alexsandro Sobreira; Coelho, Eduardo Antônio Ferraz

doi:10.1016/j.rvsc.2019.08.010

Cited by 8 publications

(3 citation statements)

References 52 publications

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“…The selected peptides were immunogenic, inducing specific T helper 1 (Th-1) response (Ramos et al, 2017;Carvalho et al, 2019). Phage display is also being used to diagnose canine leishmaniasis and as a marker for human visceral leishmaniasis (Costa et al, 2019;Machado et al, 2019). In T. cruzi, the phage display contributed to elucidate the interaction of glycoproteins from the trans-sialidase/gp85 family with mammalian host cells.…”

Section: Discussionmentioning

confidence: 99%

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The Screen of a Phage Display Library Identifies a Peptide That Binds to the Surface of Trypanosoma cruzi Trypomastigotes and Impairs Their Infection of Mammalian Cells

Paula¹,

Lopes-Torres

Jacobs-Lorena

et al. 2022

Front. Microbiol.

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BackgroundChagas is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi. On the order of seven million people are infected worldwide and current therapies are limited, highlighting the urgent need for new interventions. T. cruzi trypomastigotes can infect a variety of mammalian cells, recognition and adhesion to the host cell being critical for parasite entry. This study focuses on trypomastigote surface ligands involved in cell invasion.MethodsThree selection rounds of a phage peptide display library for isolation of phages that bind to trypomastigotes, resulted in the identification of the N3 dodecapeptide. N3 peptide binding to T. cruzi developmental forms (trypomastigotes, amastigotes and epimastigotes) was evaluated by flow cytometry and immunofluorescence assays. Parasite invasion of Vero cells was assessed by flow cytometry and immunofluorescence assays.ResultsPhage display screening identified the N3 peptide that binds preferentially to the surface of the trypomastigote and amastigote infective forms as opposed to non-infective epimastigotes. Importantly, the N3 peptide, but not a control scrambled peptide, inhibits trypomastigote invasion of Vero cells by 50%.ConclusionThe N3 peptide specifically binds to T. cruzi, and by doing so, inhibits Vero cell infection. Follow-up studies will identify the molecule on the parasite surface to which the N3 peptide binds. This putative T. cruzi ligand may advance chemotherapy design and vaccine development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Screen of a Phage Display Library Identifies a Peptide That Binds to the Surface of Trypanosoma cruzi Trypomastigotes and Impairs Their Infection of Mammalian Cells

Paula¹,

Lopes-Torres

Jacobs-Lorena

et al. 2022

Front. Microbiol.

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“…Additionally, the detection of antigens in asymptomatic dogs could lead to the identification of more assertive vaccine candidates. Machado et al (79) reviewed the advances in CanL antigen identification using immunoproteomics and phage display and explored the results obtained with these technologies and the validation of some of the identified hypothetical proteins. Briefly, to date, immunoproteomic approaches have been performed with protein extracts from the amastigotes and promastigotes of L. infantum and L. chagasi in reactions against sera of asymptomatic and symptomatic VL dogs from different regions of the world (14,42,80,81).…”

Section: Immunoproteomics For Canine Leishmaniasismentioning

confidence: 99%

Immunoproteomics and phage display in the context of leishmaniasis complexity

2023

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Leishmaniasis is defined as a complex of diseases caused by protozoan parasites of the genus Leishmania, which comprises 20 parasite species pathogenic to mammalians, such as humans and dogs. From a clinical point of view, and considering the diversity and biological complexity of the parasites, vectors, and vertebrate hosts, leishmaniasis is classified according to the distinct clinical manifestations, such as tegumentary (involving the cutaneous, mucosal, and cutaneous-diffuse forms) and visceral leishmaniasis. Many issues and challenges remain unaddressed, which could be attributed to the complexity and diversity of the disease. The current demand for the identification of new Leishmania antigenic targets for the development of multicomponent-based vaccines, as well as for the production of specific diagnostic tests, is evident. In recent years, biotechnological tools have allowed the identification of several Leishmania biomarkers that might potentially be used for diagnosis and have an application in vaccine development. In this Mini Review, we discuss the different aspects of this complex disease that have been addressed by technologies such as immunoproteomics and phage display. It is extremely important to be aware of the potential applications of antigens selected in different screening context, so that they can be used appropriately, so understanding their performance, characteristics, and self-limitations.

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Novel multi-epitope protein containing conserved epitopes from different Leishmania species as potential vaccine candidate: Integrated immunoinformatics and molecular dynamics approach

Ropón-Palacios

Zuta

Otazu

et al. 2019

Computational Biology and Chemistry

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Diagnostic markers selected by immunoproteomics and phage display applied for the serodiagnosis of canine leishmaniosis

Cited by 8 publications

References 52 publications

The Screen of a Phage Display Library Identifies a Peptide That Binds to the Surface of Trypanosoma cruzi Trypomastigotes and Impairs Their Infection of Mammalian Cells

The Screen of a Phage Display Library Identifies a Peptide That Binds to the Surface of Trypanosoma cruzi Trypomastigotes and Impairs Their Infection of Mammalian Cells

Immunoproteomics and phage display in the context of leishmaniasis complexity

Novel multi-epitope protein containing conserved epitopes from different Leishmania species as potential vaccine candidate: Integrated immunoinformatics and molecular dynamics approach

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