Diagnostic biosensors for coronaviruses and recent developments

Eissa, Shimaa

doi:10.1016/b978-0-12-824494-4.00008-4

Cited by 4 publications

(4 citation statements)

References 73 publications

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“…However, designing the sensors in the portable form for the point-of-care application is still challenging. On the other hand, miniaturized electrochemical biosensors are performing well in terms of sensitivity, accuracy, and selectivity [ 131 ]. Development of wireless micro-/nanoelectrochemical biosensors would be an ideal solution for detection COVID-19 infection.…”

Section: Challenges and Future Prospectivementioning

confidence: 99%

Advances in Biosensing Technologies for Diagnosis of COVID-19

et al. 2022

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The COVID-19 pandemic has severely impacted normal human life worldwide. Due to its rapid community spread and high mortality statistics, the development of prompt diagnostic tests for a massive number of samples is essential. Currently used traditional methods are often expensive, time-consuming, laboratory-based, and unable to handle a large number of specimens in resource-limited settings. Because of its high contagiousness, efficient identification of SARS-CoV-2 carriers is crucial. As the advantages of adopting biosensors for efficient diagnosis of COVID-19 increase, this narrative review summarizes the recent advances and the respective reasons to consider applying biosensors. Biosensors are the most sensitive, specific, rapid, user-friendly tools having the potential to deliver point-of-care diagnostics beyond traditional standards. This review provides a brief introduction to conventional methods used for COVID-19 diagnosis and summarizes their advantages and disadvantages. It also discusses the pathogenesis of COVID-19, potential diagnostic biomarkers, and rapid diagnosis using biosensor technology. The current advancements in biosensing technologies, from academic research to commercial achievements, have been emphasized in recent publications. We covered a wide range of topics, including biomarker detection, viral genomes, viral proteins, immune responses to infection, and other potential proinflammatory biomolecules. Major challenges and prospects for future application in point-of-care settings are also highlighted.

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Section: Challenges and Future Prospectivementioning

confidence: 99%

Advances in Biosensing Technologies for Diagnosis of COVID-19

et al. 2022

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“…However, there are still some limitations associated with electrochemical immunosensors regarding their sensitivity and selectivity. In order to overcome these drawbacks, new recognition sites, e.g., peptides designed to specifically bind to different types of viruses and some carbon and metal nanomaterials, have been integrated into immunosensor devices [ [3] , [4] , [5] ]. Designed peptides have stood out in the development of diagnostic platforms due to their specificity, selectivity, and versatility in the interaction with various solid surfaces and other molecules [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Gold-binding peptide as a selective layer for electrochemical detection of SARS-CoV-2 antibodies

Braz

Santiani

Martins

et al. 2023

Talanta

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“…Biomaterial-mediated reactions usually show key-lock selectivity, which is a huge advantage over other types of sensors. Therefore, these reactions have been applied as an analytical tool for many applications, e.g., biomedical, environmental, food safety, drugs, and diagnostic applications for infectious disease, among others [ 1 , 2 ]. Biosensors that use immunological reactions as a mechanism for recognizing a target, either biological or not, are classified as immunosensors.…”

Section: Introductionmentioning

confidence: 99%

“…Among them, reduced graphene oxide has drawn attention over electrochemical biosensing due to its remarkable conducting properties, large surface area, and easy modification [ 16 , 17 ]. Additionally, new recognition receptors such as aptamers and specific peptides have been extensively developed for binding to specific epitopes and antibodies [ 1 , 18 , 19 ]. Peptides have excellent recognition properties, showing their potential for a variety of diagnostic purposes, as mentioned before.…”

Section: Introductionmentioning

confidence: 99%

Graphene-Binding Peptide in Fusion with SARS-CoV-2 Antigen for Electrochemical Immunosensor Construction

et al. 2022

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The development of immunosensors to detect antibodies or antigens has stood out in the face of traditional methods for diagnosing emerging diseases such as the one caused by the SARS-CoV-2 virus. The present study reports the construction of a simplified electrochemical immunosensor using a graphene-binding peptide applied as a recognition site to detect SARS-CoV-2 antibodies. A screen-printed electrode was used for sensor preparation by adding a solution of peptide and reduced graphene oxide (rGO). The peptide-rGO suspension was characterized by scanning electron microscopy (SEM), Raman spectroscopy, and Fourier transform infrared spectroscopy (FT-IR). The electrochemical characterization (electrochemical impedance spectroscopy—EIS, cyclic voltammetry—CV and differential pulse voltammetry—DPV) was performed on the modified electrode. The immunosensor response is based on the decrease in the faradaic signal of an electrochemical probe resulting from immunocomplex formation. Using the best set of experimental conditions, the analytic curve obtained showed a good linear regression (r2 = 0.913) and a limit of detection (LOD) of 0.77 μg mL−1 for antibody detection. The CV and EIS results proved the efficiency of device assembly. The high selectivity of the platform, which can be attributed to the peptide, was demonstrated by the decrease in the current percentage for samples with antibody against the SARS-CoV-2 S protein and the increase in the other antibodies tested. Additionally, the DPV measurements showed a clearly distinguishable response in assays against human serum samples, with sera with a response above 95% being considered negative, whereas responses below this value were considered positive. The diagnostic platform developed with specific peptides is promising and has the potential for application in the diagnosis of other infections that lead to high antibody titers.

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Diagnostic biosensors for coronaviruses and recent developments

Cited by 4 publications

References 73 publications

Advances in Biosensing Technologies for Diagnosis of COVID-19

Advances in Biosensing Technologies for Diagnosis of COVID-19

Gold-binding peptide as a selective layer for electrochemical detection of SARS-CoV-2 antibodies

Graphene-Binding Peptide in Fusion with SARS-CoV-2 Antigen for Electrochemical Immunosensor Construction

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