Diagnosis of the pulmonary tuberculosis by polymerase chain reaction: a comparative study between HIV - positive and - negative individuals

Silva, Rosemeri Maurici da; Machado, Tairone; Bazzo, Maria Luiza

doi:10.1590/s1517-83822012000100030

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…Our findings are in keeping with previously published reports suggesting that traditional microbiologic tests are still effective to diagnose the TB/HIV co-infection (20)(21)(22), which highlights the importance of sample quality and their systematic study.…”

Section: Discussionsupporting

confidence: 92%

“…The median hospital stay was 19 days (IQR: [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28], and the in-hospital mortality was 11.3% (20 of 177). According to registers of the local health information systems, at the end of February of 2015, 61 of the patients included in this study had died (34.3%).…”

Section: Risk Factors Associated With Mortalitymentioning

confidence: 99%

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Current characteristics of tuberculosis and human immunodeficiency virus co-infection in a cohort of hospitalized patients in Medellín, Colombia

et al. 2018

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Introducción. La tuberculosis es una causa importante de morbilidad y mortalidad en pacientes positivos para el HIV. Los métodos de diagnóstico molecular y una mayor disponibilidad del tratamiento antirretroviral en el país pueden haber cambiado las características de la infección concomitante. Objetivo. Describir la epidemiología, las características clínicas, el diagnóstico, los patrones de resistencia, los efectos secundarios de los medicamentos antituberculosos y la mortalidad, en pacientes con las dos infecciones.Materiales y métodos. Se hizo un estudio retrospectivo basado en la revisión de historias clínicas de adultos hospitalizados en un hospital universitario de Medellín, Colombia.Resultados. Se incluyeron 178 pacientes en el estudio. El diagnóstico de tuberculosis e infección por el HIV fue simultáneo en 49,9 %. En el momento del diagnóstico, la mediana de CD4 fue de 61 células/μL (rango de 27 a 145). La tuberculosis pulmonar ocurrió en 28 % de los pacientes, la extrapulmonar en 23% y la mixta en 48,9%. En la tuberculosis extrapulmonar, el compromiso fue principalmente linfático (55,4 %), gastrointestinal (35,9%) y del sistema nervioso central (18,7 %). La tinción de Ziehl-Neelsen fue positiva en 137 pacientes (77 %), en tanto que el cultivo para micobacterias lo fue en 121 (68 %). La reacción en cadena de la polimerasa para detectar la tuberculosis fue positiva en 85 de los pacientes a quienes se les hizo la prueba. Se detectó resistencia a la rifampicina en seis casos (4,9 %). Al iniciar el tratamiento antituberculoso, las transaminasas se elevaron en la mitad de los pacientes, pero solo 10 % cumplieron los criterios de hepatotoxicidad. La mortalidad hospitalaria fue de 11,3 %. El único factor de riesgo asociado con la mortalidad fue un conteo de CD4 menor de 50/μL (RR=3,9; IC95% 1,36-11,37; p=0,01).Conclusiones. Cuando la tuberculosis se presenta de manera oportunista, comúnmente lleva al diagnóstico de enfermedad avanzada por el HIV. Su diagnóstico en estos pacientes puede hacerse con los métodos convencionales. Es necesario vigilar la función hepática durante el tratamiento y excluir la resistencia a los medicamentos.

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Section: Discussionsupporting

confidence: 92%

Section: Risk Factors Associated With Mortalitymentioning

confidence: 99%

Current characteristics of tuberculosis and human immunodeficiency virus co-infection in a cohort of hospitalized patients in Medellín, Colombia

et al. 2018

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“…The supernatant is decanted and transferred to the sediment in 2 ml of phosphate buffer pH 6.8. The sediment is used for ZN staining and DNA extraction [13,14].…”

Section: Methodsmentioning

confidence: 99%

To Analyze the Tb-PCR Positivity Rate Using Real-Time PCR for Early Detection of Tuberculosis

Sawant

Sharma

et al. 2023

Asian J Pharm Clin Res

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Objective: Tuberculosis is one of the leading causes of death worldwide. It is present in all the countries and among all age groups and also seen in both the genders. In investigating the early stage, identification, and rapid detection of tuberculosis, the PCR method (polymerase chain reaction) is one of the fast, safest, and reproducible new approaches. It covers all advantages such as the use of closed systems, reduced risk of carryover contamination, improved sensitivity and reproducibility, reduced turnaround time wide dynamic range of target detection, and feasibility for quantitation making it easy and reliable for the early detection of Mycobacterium tuberculosis. Methods: All samples used for the study are confirmed by conventional microscopic observation using acid-fast staining using ZN STAIN. The pulmonary sputum samples are obtained from clinical and radiological evidence of tuberculosis and these samples are selected for DNA extraction. Discussion: As per the statistical analysis using SPSS 22 version, it is found that the TB positivity rate (is 29.75%). Out of 119 positive patients (Male: 60.5% and Female: 39.5%). The average and standard deviation for CT values are 23.6 and 2.9, respectively. The 95% confidence interval of CT values for specimens is (22.7, 24.5). Average and standard deviation for CT values are equal in male patients and female patients. Age group-wise average and standard deviation for CT values are nearly equal. Results: The percentage of 3+ AFB positive grades having CT values between 20 and 24 is more than all other combinations of AFB positive grades and CT values. The average and standard deviation for CT values are 23.6 and 2.9, respectively. The 95% confidence interval of CT values for specimens is (22.7, 24.5). The average and standard deviation for CT values are equal in male patients and female patients. Conclusion: A significant improvement in specificity with high accuracy was achieved by a real-time PCR assay. Real-time PCR tests prove both a high degree of sensitivity in the upper respiratory sputum samples and for the early detection of the TB infection. The MTB real-time PCR test suitably identified the majority of the AFB positive and bacterial culture confirms TB.

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“…Однако, согласно другим исследованиям, мо-лекулярно-генетические методы детекции МБТ у больных ВИЧ-инфекцией обладают меньшей ин-формативностью [9].…”

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Detection of Tuberculous Mycobacteria in Sputum and Intensity of Bacillary Excretion in Tuberculosis Patients With Various Hiv Status

Zimina¹,

Mikova²,

Varetskaya³

et al. 2017

TaLD

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1ФГАОУ ВО «Российский университет дружбы народов», Москва, Россия 2 ГКУЗ ПК «Пермский краевой центр по профилактике и борьбе со СПИД и инфекционными заболеваниями», г. Пермь, Россия 3 ГБОУ ВПО «Пермский государственный медицинский университет им. акад. Е. А. Вагнера», г. Пермь, Россия Цель исследования: изучить информативность выявления микобактерий туберкулеза (МБТ) различными методами и провести анализ массивности бактериовыделения у больных туберкулезом в зависимости от ВИЧ-статуса.Материалы и методы. Обследовано 360 больных ВИЧ-инфекцией с подозрением на туберкулез. Диагностический алгоритм включал двукрат-ное исследование мокроты на кислотоустойчивые микобактерии (КУМ)/МБТ люминесцентной микроскопией, молекулярно-генетическим методом и посевом на жидкие и плотные питательные среды. Туберкулез установлен 178 (49,4%) больным. Группу сравнения составили 354 пациента с туберкулезом без ВИЧ-инфекции, в том числе 113 пациентов, обследование которых включало полный диагностический алгоритм, как у больных коинфекцией.Результаты исследования. У больных туберкулезом без ВИЧ-инфекции чаще удавалось выявить КУМ методом люминесцентной микроско-пии, чем у пациентов с коинфекцией (32,8 и 24,2% соответственно), при этом чувствительность остальных методов не различалась. У больных коинфекцией достоверно чаще регистрировали скудное и реже -обильное бактериовыделение методом посева на плотные среды, чем у больных туберкулезом без ВИЧ-инфекции. Полный диагностический алгоритм продемонстрировал лучшую чувствительность у больных коинфекцией в сравнении с больными туберкулезом без ВИЧ-инфекции (87,1 и 71,2%). results of the study. AFB were more often detected by fluorescent microscopy in HIV negative tuberculosis patients compared to those with TB/HIV co-infection (32.8 and 24.2% respectively), and the sensitivity of the other testing methods did not differ. Scarce bacillary excretion was confidently more often registered in those suffering from TB/HIV co-infection and less frequently they had massive bacillary excretion detected by culture on solid media compared to HIV negative tuberculosis patients. The complete diagnostic procedure demonstrated better sensitivity in those with TB/HIV co-infection compared to tuberculosis patients with negative HIV status (87.1 and 71.2%). Key words: tuberculosis, co-infection, HIV infection, intensity of bacillary excretion, informativeness of tuberculous mycobacteria detectionFor citations: Zimina V.N., Mikova O.E., Varetskaya T.А., Degtyareva S.Yu., Sergevnin V.I. Detection of tuberculous mycobacteria in sputum and intensity of bacillary excretion in tuberculosis patients with various HIV status. Tuberculosis and Lung Diseases, 2017, Vol. 95, no. 7, P. 17-23. (In Russ.) DOI: 10.21292/2075-1230-2017 Всемирная организация здравоохранения и Рос-сийское общество фтизиатров рекомендуют обсле-довать на туберкулез людей, живущих с ВИЧ, при выявлении как минимум одного из четырех клини-ческих симптомов: кашля; лихорадки; ночной по-тливости; потери массы тела. Считается, что при отсутствии всех обо...

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Diagnosis of the pulmonary tuberculosis by polymerase chain reaction: a comparative study between HIV - positive and - negative individuals

Cited by 6 publications

References 7 publications

Current characteristics of tuberculosis and human immunodeficiency virus co-infection in a cohort of hospitalized patients in Medellín, Colombia

Current characteristics of tuberculosis and human immunodeficiency virus co-infection in a cohort of hospitalized patients in Medellín, Colombia

To Analyze the Tb-PCR Positivity Rate Using Real-Time PCR for Early Detection of Tuberculosis

Detection of Tuberculous Mycobacteria in Sputum and Intensity of Bacillary Excretion in Tuberculosis Patients With Various Hiv Status

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