Diagnosis of shiso mosaic disease using a reverse transcription loop-mediated isothermal amplification assay

Abstract

We developed a technique to detect Perilla mosaic virus (PMoV), a pathogenic virus causing perilla mosaic disease, using the reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Although PMoV is widely distributed in Japan and is known to be composed two major clades on the basis of nucleotide sequences, all of the isolates tested were readily detected, by mixing two kinds of FIP and BIP primers corresponding to each clade. In addition, PMoV could be detected with an accuracy of 92.3% even with a simple template obtained by piercing symptomatic leaves with a needle. Furthermore, even in places where electric power is not available, PMoV could be detected on site by performing an RT-LAMP reaction using an automobile battery.