Diagnosis of Rickettsioses from Eschar Swab Samples, Algeria

Mouffok, Nadjet; Socolovschi, Cristina; Benabdellah, A.; Renvoisé, Aurélie; Parola, Philippe; Raoult, Didier

doi:10.3201/eid1710.110332_article.htm

Cited by 8 publications

(13 citation statements)

References 5 publications

(7 reference statements)

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“…As expected, most health professionals preferred collecting swab samples over biopsy samples for patients and for themselves. Patients also preferred having a swab sample taken over a skin biopsy sample (471). Swabbing of an eschar is easy and painless.…”

Section: New Approaches To Diagnosismentioning

confidence: 99%

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Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

et al. 2013

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SUMMARY Tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus Rickettsia . These zoonoses are among the oldest known vector-borne diseases. However, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. Several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections, and novel Rickettsia species of undetermined pathogenicity continue to be detected in or isolated from ticks around the world. This remarkable expansion of information has been driven largely by the use of molecular techniques that have facilitated the identification of novel and previously recognized rickettsiae in ticks. New approaches, such as swabbing of eschars to obtain material to be tested by PCR, have emerged in recent years and have played a role in describing emerging tick-borne rickettsioses. Here, we present the current knowledge on tick-borne rickettsiae and rickettsioses using a geographic approach toward the epidemiology of these diseases.

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Section: New Approaches To Diagnosismentioning

confidence: 99%

“…Eschar swabbing has also been successfully used when a total of 39 patients were sampled in Oran, Algeria (471). Swabs were then sent to and tested in Marseille, France.…”

Section: New Approaches To Diagnosismentioning

confidence: 99%

Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

et al. 2013

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“…Rickettsial DNA may be present in the crusts and fluids of the eschars, even after the initiation of treatment and in cases for which serum (even convalescent) remains negative against rickettsial antigens. 11 The usefulness of cotton swab PCR testing for such diagnoses has been shown for Queensland tick typhus and African tick bite fever, 11 scrub typhus, 12 Mediterranean spotted fever, and lymphangitisassociated rickettsiosis 13,14 and R. parkeri infection. 15 Here, we present data regarding the identification of bacterial DNA in swabs obtained from eschars from acute febrile patients in Senegal.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Pathogenic Bacteria in Eschars from Acute Febrile Patients, Senegal

Mediannikov

Socolovschi

Million

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Fever caused by Rickettsia felis was recently shown to play an important role in infectious diseases morbidity in sub-Saharan Africa. We collected 68 cotton swabs from fever-associated eschars in four different regions of Senegal. In 5 of 68 eschar samples (7.4%), we have identified DNA from R. felis. In 49 of 68 eschar samples (72.1%), we have identified DNA from Staphylococcus aureus. In 35 of 68 eschar samples (51.5%), we have identified DNA from Streptococcus pyogenes, and in 4 of 68 eschar samples (5.9%), we have identified DNA from Streptococcus pneumoniae. In 34 cases, S. aureus was found together with S. pyogenes. DNA from R. felis was also found in swabs from the skin of the healthy Senegalese villagers (3 of 60; 5%) but not French urbanites. The presence of S. aureus and S. pyogenes was significantly associated with the presence of eschars in febrile patients compared with the healthy skin from the control group. Finally, we confirmed that Senegal is an endemic region for R. felis, which is found in both eschars and healthy skin swabs.

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“…Recently, real-time PCR gain sensitivity and specificity by using new generation primers (Renvoise et al, 2012). Molecular detection was simplified by using directly the eschar swabs samples (Mouffok et al, 2011). ELISA (Fuller Laboratories) methods are also currently available for non-referenced laboratories which are focused on more specific reactivities to the lipopolysaccharide (LPS) antigens (IgG screening) and rOmpB protein antigens (IgG/IgM).…”

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Detection Of Immunogenic Proteins From Rickettsia conorii And Rickettsia africae By Proximity Ligation Assay Based Western Blotting (PLA WB)

N’Djatchi¹,

Bagre²,

Kra³

et al. 2019

Indian Journal of Scientific Research

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Rickettsia conorii, the causative agent of Mediterranean spotted fever (MSF) and Rickettsia africae, the etiologic agent of African tick-bite fever (ATBF), are strict intracellular bacteria transmitted by ticks belonging to the spotted fever group (SFG) Rickettsia. The laboratory diagnosis is based on serology, but remains not enough specific to provide the diagnosis at the species level. Here, we attempted to detecty immunogenic proteins specific from Rickettsia conorii and Rickettsia africae by proximity ligation assay based Western blotting (PLA WB). In general, we obtained better sensitivity and specificity of bands detection in PLA WB in case of proteins probed with IFA R. conorii or R. africae positive sera. In parallel, we have screened 20 rickettsial recombinant proteins by ELISA with sera infected by R. conorii and R. africae, respectively. We identified several potential markers which allowed infection due to R. conorii to be discriminated from those due to R. africae. Never the less, this technique must be optimized for use in laboratory diagnostic routine.

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Diagnosis of Rickettsioses from Eschar Swab Samples, Algeria

Cited by 8 publications

References 5 publications

Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

Molecular Identification of Pathogenic Bacteria in Eschars from Acute Febrile Patients, Senegal

Detection Of Immunogenic Proteins From Rickettsia conorii And Rickettsia africae By Proximity Ligation Assay Based Western Blotting (PLA WB)

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