Molecular Identification of Pathogenic Bacteria in Eschars from Acute Febrile Patients, Senegal

Mediannikov, Oleg; Socolovschi, Cristina; Million, Matthieu; Sokhna, Cheikh; Basséne, Hubert; Diatta, Georges; Fénollar, Florence; Raoult, Didier

doi:10.4269/ajtmh.13-0629

Cited by 27 publications

(21 citation statements)

References 27 publications

(27 reference statements)

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“…3,4,12,16 However, as R. felis DNA has also been detected on the skin of healthy Senegalese villagers and in the feces of several arthropod vectors such as Ctenocephalides felis or Liposcelis bostrychophila, it is still unclear to which extent these findings may be influenced by skin contamination. 5,12,17 In conclusion, our study is the first report on the detection of R. felis in febrile children in Ghana, and thus adds to the growing evidence for a widespread occurrence of R. felis in SSA. To better understand the pathogenesis of R. felis infections, future studies should longitudinally monitor the presence of R. felis DNA and specific antibodies following infection, and use control groups to determine the clinical significance of R. felis, both in the absence and presence of malaria coinfection as well as in asymptomatic patients.…”

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confidence: 62%

Rickettsia felis Infection in Febrile Children, Ghana

et al. 2017

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Abstract. Rickettsial infections are an underrecognized cause of febrile illness in sub-Saharan Africa. To evaluate the epidemiology and clinical features of rickettsial disease in pediatric patients in Ghana, we screened blood samples from febrile children aged less than 15 years presenting to an outpatient department in Ghana's Ashanti Region for the presence of rickettsial DNA. We detected Rickettsia felis in 7/470 (1.5%) blood samples, using two independent real-time polymerase chain reactions. No other Rickettsia species were found. R. felis was detected repeatedly in one patient, and coinfection with Plasmodium falciparum was found in 3/7 samples. Symptoms apart from fever included cough (6/7) and vomiting (4/7). None of the R. felis-positive patients reported a rash. This study is the first report on R. felis in Ghana and adds to the growing evidence for its widespread occurrence with and without malaria coinfection in sub-Saharan Africa.

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confidence: 62%

Rickettsia felis Infection in Febrile Children, Ghana

et al. 2017

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“…(more particularly Rickettsia felis and Rickettsia africae) are frequent causes of tropical fever. They can be detected by appropriate reported but noncommercialized RT-PCR assays (4,(33)(34)(35). A nanogold particle lateral flow assay was recently reported for POC diagnosis of dengue virus, yellow fever virus, and Ebola virus infections (23).…”

Section: Tropical Fevermentioning

confidence: 99%

The Point-of-Care Laboratory in Clinical Microbiology

et al. 2016

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International audiencePoint-of-care (POC) laboratories that deliver rapid diagnoses of ă infectious diseases were invented to balance the centralization of core ă laboratories. POC laboratories operate 24 h a day and 7 days a week to ă provide diagnoses within 2 h, largely based on immunochromatography and ă real-time PCR tests. In our experience, these tests are conveniently ă combined into syndrome-based kits that facilitate sampling, including ă self-sampling and test operations, as POC laboratories can be operated ă by trained operators who are not necessarily biologists. POC ă laboratories are a way of easily providing clinical microbiology testing ă for populations distant from laboratories in developing and developed ă countries and on ships. Modern Internet connections enable support from ă core laboratories. The cost-effectiveness of POC laboratories has been ă established for the rapid diagnosis of tuberculosis and sexually ă transmitted infections in both developed and developing countries

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“…Apparent difference in the prevalence of R. felis by study site may be related to number of cats/dogs associated with population and its density in individual divisions, although a definite reservoir of this Rickettsia has not yet been established. Recently, detection of R. felis DNA was reported in the Anopheles gambiae mosquito [4] , and also in blood and skin of healthy individuals [6] , [11] , [12] , suggesting a capability of R. felis to colonize or infect various living organisms. Therefore, further studies are necessary to determine definite pathogenic significance of R. felis DNA in humans, and also to explore the potential reservoir and vector of this bacterium, in a putative endemic area such as Bangladesh.…”

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confidence: 99%