Diagnosis of Helicobacter pylori infection in dyspeptic patients by stool antigen detection

Calvet, Xavier; Quesada, Mariela; Sanfeliú, Isabel; Salceda, F.; Roselló, M.; Montserrat, A.; Brullet, Enric; Segura, Ferrán

doi:10.1016/j.dld.2004.01.025

Cited by 22 publications

(13 citation statements)

References 20 publications

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“…However, the main differences are in specificity, while the specificity obtained by HpStAR was 70.7%, while HpSA specificity was 80.5%. Similar results were obtained by Calvet et al 25 who reported, in a evalua-tion of the HpSA and the FemtoLab tests using the faeces of dyspeptic patients, that there was a specificity of 76% using FemtoLab and 86% using HpSA. Previously, the same authors 26 had evaluated these kits in cirrhotic patients, obtaining specificities for HpSA and FemtoLab of 93% and 79%, respectively.…”

Section: Discussionsupporting

confidence: 87%

Comparison of a monoclonal with a polyclonal antibody‐based enzyme immunoassay stool test in diagnosing Helicobacter pylori infection before and after eradication therapy

Domínguez

Forné

Blanco

et al. 2006

Aliment Pharmacol Ther

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Section: Discussionsupporting

confidence: 87%

Comparison of a monoclonal with a polyclonal antibody‐based enzyme immunoassay stool test in diagnosing Helicobacter pylori infection before and after eradication therapy

Domínguez

Forné

Blanco

et al. 2006

Aliment Pharmacol Ther

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“…Stool specimens were received within 24 hr after collection and stored at -20 • C until tested for H. pylori antigen by the FemtoLab, Commex, Germany, a sandwich enzyme immunoassay, in accordance with the manufacturer's instructions [19]. Microtiter plate wells were coated with monoclonal antibodies for H. pylori antigen.…”

Section: H Pylori Antigen Detectionmentioning

confidence: 99%

Helicobacter pylori and Clostridium difficile in Cystic Fibrosis Patients

et al. 2006

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We describe the prevalence of H. pylori and toxigenic Clostridium difficile (CD) infection and its relationship with gastrointestinal symptoms and pancreatic sufficiency (PS) or insufficiency (PI) in cystic fibrosis (CF) patients. Stool specimens from 30 consecutive patients with CF, aged 1-44, and from 30 healthy similarly aged subjects were tested for the H. pylori antigen by specific monoclonal antibodies and for CD toxins by Tox A/B assay and Tox A assay. CF patients were assessed clinically and tested for specific H. pylori serum antibodies and for mutations. In CF patients, the prevalence of H. pylori antigen was 16.6% (5/30), compared to 30% (9/30) in controls. Of the 26 CF patients with PI, only 2 (7.6%) were infected by H. pylori, compared with 3 of the 4 (75%) patients with PS (P=0.001). H. pylori infection was diagnosed in 3 of 5 (60%) CF patients carrying mild mutations, compared to 1 of 25 (4%) CF patients carrying severe mutations (P=0.01). Fourteen of 30 (46.6%) stool specimens from CF patients tested positive in the ToxA/B assay, and 3 of 14 tested positive for ToxA. No significant differences in antibiotic use, severity of lung disease, PI, chronic abdominal pain, or genotype were found between the two groups. None of the controls was positive for CD toxins. Prevalence of H. pylori infection in CF patients was lower than in similarly aged non-CF controls. CF patients with PI or a history of distal intestinal obstruction syndrome and those carrying mutations associated with a severe phenotype were protected against H. pylori infection. Almost half of the CF patients were asymptomatic carriers of CD producing mostly toxin B. More studies are needed to confirm our results in a larger group of CF patients.

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“…In this study, the sensitivity of PCR from stool specimens exceeds that of previously reported attempts [13]. Therefore, stool PCR may be considered as better test than HpSA because the HpSA test shows heterogeneity in the sensitivity rates that ranged from 58% to 96% and specificity ranged from 67% to96 % [14][15][16][17][18][19][20][21][22]. This is because of the difficulty in obtaining polyclonal antibodies of constant quality [23].…”

Section: Discussionmentioning

confidence: 52%

Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection

Mishra

Singh

Jain

et al. 2008

J Infect Dev Ctries

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Background: Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. Methodology: A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during preand post-therapy periods for rapid urease test (RUT) and PCR. Similarly stool specimens for PCR and HpSA test were collected during both the periods from all 52 patients. Biopsy, PCR and RUT results together were considered the "gold standard." Results: On the basis of gold standard tests, 40/52 patients were H. pylori positive. The sensitivity and specificity of HpSA test were 80% and 83.3% respectively in untreated patients. On the other hand, the sensitivity and specificity of stool PCR in untreated patients were 72.5% and 100% respectively. After eradication therapy, the results of both RUT and biopsy PCR were negative in 87.5% and positive in 12.5% of the patients. Although post treatment sensitivity of HpSA and stool PCR was equal (60%), specificity of HpSA and stool PCR were 68.6% and 97.1% respectively. Conclusion: The H. pylori stool tests represent a non-invasive concept for diagnosis of infection. Both HpSA and stool PCR seem to be satisfactory tests for pre-eradication as well as assessment of infection. But stool PCR is a better indicator than HpSA test in the post-eradication assessment of infection.

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Diagnosis of Helicobacter pylori infection in dyspeptic patients by stool antigen detection

Cited by 22 publications

References 20 publications

Comparison of a monoclonal with a polyclonal antibody‐based enzyme immunoassay stool test in diagnosing Helicobacter pylori infection before and after eradication therapy

Comparison of a monoclonal with a polyclonal antibody‐based enzyme immunoassay stool test in diagnosing Helicobacter pylori infection before and after eradication therapy

Helicobacter pylori and Clostridium difficile in Cystic Fibrosis Patients

Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection

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