Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection

Abstract

Background: Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. Methodology: A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during preand post-therapy periods for rapid urease test (RUT) and PCR. Similarly stool specimens for PCR and HpSA test were collected during both the periods from all 52 patients. Biopsy, PCR and RUT results together were considered the "gold standard." Results: On the basis of gold standard tests, 40/52 patients were H. pylori positive. The sensitivity and specificity of HpSA test were 80% and 83.3% respectively in untreated patients. On the other hand, the sensitivity and specificity of stool PCR in untreated patients were 72.5% and 100% respectively. After eradication therapy, the results of both RUT and biopsy PCR were negative in 87.5% and positive in 12.5% of the patients. Although post treatment sensitivity of HpSA and stool PCR was equal (60%), specificity of HpSA and stool PCR were 68.6% and 97.1% respectively. Conclusion: The H. pylori stool tests represent a non-invasive concept for diagnosis of infection. Both HpSA and stool PCR seem to be satisfactory tests for pre-eradication as well as assessment of infection. But stool PCR is a better indicator than HpSA test in the post-eradication assessment of infection.

Diagnostic Values of Multiplex Loop-Mediated Isothermal Amplification and Multiplex Polymerase Chain Reaction for Detection of Methicillin-Resistant Staphylococcus aureus

“…The routine detection methods of MRSA, like culture and PCR, have several strengths and weaknesses. The disadvantages of these techniques include the high cost of the instruments, and more importantly, the inability to apply as a point of care test (15,16). Thus, the development of a low-cost and fast method with high sensitivity and specificity is of utmost importance (17,18).…”