Diagnosis of EGFR exon21 L858R point mutation as lung cancer biomarker by electrochemical DNA biosensor based on reduced graphene oxide /functionalized ordered mesoporous carbon/Ni-oxytetracycline metallopolymer nanoparticles modified pencil graphite electrode

Shoja, Yalda; Kermanpur, A.; Karimzadeh, F.

doi:10.1016/j.bios.2018.04.013

Cited by 62 publications

(18 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensitivity is compared with a recently reported work. It is found that the genosensor sensitivity is 16 fold higher compared to the proposed electrochemical DNA biosensor in EGFR mutant detection 44 . Therefore, it can be concluded that the proposed genosensor exhibits significant sensitivity due to the excellent physiochemical characteristics of aluminosilicates, further followed by precise surface chemistry in the probe immobilization which creates a huge density of DNA hybridization on genosensor.…”

Section: Resultsmentioning

confidence: 91%

Aluminosilicate Nanocomposite on Genosensor: A Prospective Voltammetry Platform for Epidermal Growth Factor Receptor Mutant Analysis in Non-small Cell Lung Cancer

Ramanathan

Arshad

Poopalan

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Lung cancer is one of the most serious threats to human where 85% of lethal death caused by non-small cell lung cancer (NSCLC) induced by epidermal growth factor receptor (EGFR) mutation. The present research focuses in the development of efficient and effortless EGFR mutant detection strategy through high-performance and sensitive genosensor. The current amplified through 250 µm sized fingers between 100 µm aluminium electrodes indicates the voltammetry signal generated by means of the mutant DNA sequence hybridization. To enhance the DNA immobilization and hybridization, ∼25 nm sized aluminosilicate nanocomposite synthesized from the disposed joss fly ash was deposited on the gaps between aluminium electrodes. The probe, mutant (complementary), and wild (single-base pair mismatch) targets were designed precisely from the genomic sequences denote the detection of EGFR mutation. Fourier-transform Infrared Spectroscopy analysis was performed at every step of surface functionalization evidences the relevant chemical bonding of biomolecules on the genosensor as duplex DNA with peak response at 1150 cm−1 to 1650 cm−1. Genosensor depicts a sensitive EGFR mutation as it is able to detect apparently at 100 aM mutant against 1 µM DNA probe. The insignificant voltammetry signal generated with wild type strand emphasizes the specificity of genosensor in the detection of single base pair mismatch. The inefficiency of genosensor in detecting EGFR mutation in the absence of aluminosilicate nanocomposite implies the insensitivity of genosensing DNA hybridization and accentuates the significance of aluminosilicate. Based on the slope of the calibration curve, the attained sensitivity of aluminosilicate modified genosensor was 3.02E-4 A M−1. The detection limit of genosensor computed based on 3σ calculation, relative to the change of current proportional to the logarithm of mutant concentration is at 100 aM.

show abstract

Section: Resultsmentioning

confidence: 91%

Aluminosilicate Nanocomposite on Genosensor: A Prospective Voltammetry Platform for Epidermal Growth Factor Receptor Mutant Analysis in Non-small Cell Lung Cancer

Ramanathan

Arshad

Poopalan

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Moreover, the aqueous environment may damage nanomaterials, which leads to biosensors' error or disability. Finally, some of NPs or 2D materials in the composite material inhibit or interact with the biomarker, which affects the activity of biomarker or specific binding, resulting in a discrepancy of actual concentration [ 28 , 34 , 35 , 39 , 119 , 187 , 190 ].…”

Section: Discussionmentioning

confidence: 99%

“…Besides 2D materials, some researchers with biological background have brought biochemical materials into biomarker detection which have exhibited tremendous potential in the field of clinical medicine. Shoja et al fabricated a DNA biosensor with high electrical conductivity and large area of an effective surface based on rGO/functionalized mesoporous carbon/Ni-oxytetracycline metallopolymer nanoparticle-modified pencil graphite, immobilized onto the surface of electrodes [ 34 ]. Combined with chemical vapor deposition (CVD), graphene has been synthesized on Cu film and immobilized anti-CEA which linked PBSE.…”

Section: Electrochemical Biomarker Sensorsmentioning

confidence: 99%

Recent Progress of Biomarker Detection Sensors

Liu

Cui

2020

Research

View full text Add to dashboard Cite

Early cancer diagnosis and treatment are crucial research fields of human health. One method that has proven efficient is biomarker detection which can provide real-time and accurate biological information for early diagnosis. This review presents several biomarker sensors based on electrochemistry, surface plasmon resonance (SPR), nanowires, other nanostructures, and, most recently, metamaterials which have also shown their mechanisms and prospects in application in recent years. Compared with previous reviews, electrochemistry-based biomarker sensors have been classified into three strategies according to their optimizing methods in this review. This makes it more convenient for researchers to find a specific fabrication method to improve the performance of their sensors. Besides that, as microfabrication technologies have improved and novel materials are explored, some novel biomarker sensors—such as nanowire-based and metamaterial-based biomarker sensors—have also been investigated and summarized in this review, which can exhibit ultrahigh resolution, sensitivity, and limit of detection (LoD) in a more complex detection environment. The purpose of this review is to understand the present by reviewing the past. Researchers can break through bottlenecks of existing biomarker sensors by reviewing previous works and finally meet the various complex detection needs for the early diagnosis of human cancer.

show abstract

“…In electronic biosensors the probe is fixed on the surface of the electrode, and the target sequence is captured on the surface of the electrode by a specific action ( Zhang et al, 2008 ). DNA can be hybridized according to the redox characteristics of the base, adding electroactive hybridization indicator to modify the electrode or labeling enzymes ( Liu et al, 2020 ), electroactive groups and nanoparticles ( Shoja et al, 2018 ) as reporters of electrochemical activity. Hybridization is used to detect the changes in electrical signal.…”

Section: Biosensors For Point Mutation Detectionmentioning

confidence: 99%