Diagnosis of dermatophytosis: an evaluation of direct examination using MycetColor® and MycetFluo®

Pihet, Marc; Clément, Nathalie; Kauffmann‐Lacroix, C.; Nail-Billaud, Sandrine; Marot, Agnès; Pilon, Françoise; Robert, Raymond

doi:10.1016/j.diagmicrobio.2015.06.022

Cited by 14 publications

(8 citation statements)

References 18 publications

(19 reference statements)

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“…A common view among laboratory staff is that fluorescence microscopy is easier, faster and safer than bright‐field examination. This opinion is in line with a few comparative studies originating from departments of microbiology or dermatopathology . The potential advantages, however, in using fluorescence microscopy instead of light microscopy when screening for dermatophyte infections at a clinical level remains to be elucidated.…”

Section: Introductionsupporting

confidence: 71%

Dermatophytosis: fluorostaining enhances speed and sensitivity in direct microscopy of skin, nail and hair specimens from dermatology outpatients

Ovrén

Berglund

Nordlind

et al. 2016

Mycoses

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Direct microscopy of keratinised specimens is a standard screening procedure that assists clinicians to differentiate true superficial mycoses from non-fungal disorders of the skin, nail and hair. Most clinical dermatologists use bright-field microscopy when searching for dermatophyte fungi in clinical samples while laboratory-based mycologists increasingly favour fluorescence microscopy in order to optimise visualisation of fungal elements. This study compared the validity and speediness of fluorescence microscopy vs. conventional light microscopy when screening for fungi in 206 dermatological samples from dermatology outpatients. Both senior dermatologist and a less experienced investigator (medical student) attained high and comparable levels of specificity (91.7-93.8%), positive predictive value (77.1-81.4%) and negative predictive value (83.7-89.9%) using either method. Fluorostaining with Blankophor prior to fluorescence microscopy increased the sensitivity by 22 ± 1% as compared to light microscopy of unstained samples. For both investigators, the time required to identify fungal elements by the fluorescence-based technique was reduced by at least 50%, thus improving the performance of direct microscopy in the clinical setting.

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Section: Introductionsupporting

confidence: 71%

Dermatophytosis: fluorostaining enhances speed and sensitivity in direct microscopy of skin, nail and hair specimens from dermatology outpatients

Ovrén

Berglund

Nordlind

et al. 2016

Mycoses

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“…Macroconidia suspension was taken from the grounded petri dish colony and centrifuged in peptol water suspension, which was resuspended with distilled water to obtain an even clearer suspension. Macroconidia was analyzed by Olympus optical CO microscope (400x magnification), Ltda BX40 (Tokyo, Japan) [17,18]…”

Section: Preparation and Standardization Of Dermatophytesmentioning

confidence: 99%

Mechanisms of Dermatophyte Invasion on New Zealand Rabbit Skin Models

et al. 2020

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Dermatophytosis is a dermatophyte fungi infection most commonly found in animals and humans. The first step of infection is started from the attachment of arthroconidium into stratum corneum. The attachment of fungi to the host cell is mediated by fungi adhesin and its interaction with the host receptor. The objective of this research was to develop the infection model of Microsporum gypseum and Microsporum canis by inoculating the macroconidia intradermally on the rabbit model. The macroconidia collection method from culture media was re-visualized as fungi ultrastructure using scanning electron microscopy (SEM). Skin lesion analysis was measured from clinical changes of the skin based on primary dermatophytosis signs such as circular alopecia with erythema and squamosal. Clinical confirmation test was done via skin sampling followed by histopathological examination using Methenamine Silver—Grocott’s (GMS) staining. As a result of this research, the in vivo infection model through direct infection of macroconidia applied intradermally was very effective in improving the direct infection to the invasion phase on the skin. This model confirmed the epidermal differentiation process and skin permeability showed primary lesion within 2 hours and aggravated up to 6 hours after inoculation. In conclusion, macroconidium is a potential source of infection to induce the dermatophytosis model and the severity of primary injection correlated with duration and the scale of clinical symptoms exhibited. This is a promising model for further research on the mechanism involved in dermatophyte infection.

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“…[1][2][3] The fluorescent method using CFW (calcofluor white) is a simple, rapid, highly efficient direct microscopy method that has a higher positive detection rate compared with KOH in various superficial fungal infectious diseases. 4,5 Several studies have compared CFW and other methods to diagnose suspected onychomycosis, but they did not show much better results with CFW. 6,7 Besides, the biggest disadvantages of CFW are that it diminishes quickly and its relatively bright back- brightener 85), a popular and widely used fluorescent brightener in wood, washing and dyeing industry in the world, in the detection of fungi in superficial fungal infectious diseases and proved that it had a higher positive rate than the KOH method with statistically significant differences (data not published).…”

Section: Introductionmentioning

confidence: 99%

“…Several reports have shown a relatively higher diagnostic positive rate using PAS (periodic acid‐Schiff), but it has seldom been used in clinical practice due to its time‐consuming nature compared with the KOH method . The fluorescent method using CFW (calcofluor white) is a simple, rapid, highly efficient direct microscopy method that has a higher positive detection rate compared with KOH in various superficial fungal infectious diseases . Several studies have compared CFW and other methods to diagnose suspected onychomycosis, but they did not show much better results with CFW .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a new fluorescent reagent, fluorescent brightener 85, for the diagnosis of suspected onychomycosis compared with potassium hydroxide

Yue

Wang

et al. 2017

Mycoses

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Onychomycosis is a common fungal infection in dermatology clinics. The commonly used diagnostic method, potassium hydroxide (KOH) direct microscopy, does not have a high-positive detection rate. Therefore, a new diagnostic method is needed to confirm onychomycosis. The objective of this research was to use fluorescent brightener 85 (FB 85), a new fluorescent reagent for fungi detection, and to evaluate this new method based on the diagnostic positive rate, accuracy and efficiency in suspected onychomycosis compared with the KOH method. Subungual debris was collected from clinically suspected onychomycosis cases and divided into two parts. The parts were examined by FB 85 and 10% KOH solution, respectively. In total, 108 patients with suspected onychomycosis were recruited. The positive rates of FB 85 and KOH were 88.9% and 55.6%, respectively. The patients showed significant statistical differences (P < .001). Compared with the KOH method, the FB 85 fluorescent method was an accurate and efficient method with a higher positive rate. This method could increase the diagnostic accuracy and efficiency of onychomycosis detection.

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Diagnosis of dermatophytosis: an evaluation of direct examination using MycetColor® and MycetFluo®

Cited by 14 publications

References 18 publications

Dermatophytosis: fluorostaining enhances speed and sensitivity in direct microscopy of skin, nail and hair specimens from dermatology outpatients

Dermatophytosis: fluorostaining enhances speed and sensitivity in direct microscopy of skin, nail and hair specimens from dermatology outpatients

Mechanisms of Dermatophyte Invasion on New Zealand Rabbit Skin Models

Evaluation of a new fluorescent reagent, fluorescent brightener 85, for the diagnosis of suspected onychomycosis compared with potassium hydroxide

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