Highly pathogenic avian influenza (HPAI) A(H5N1) viruses have been circulating since 2003 in Indonesia, with major impacts on poultry health, severe economic losses, and 168 fatal laboratory-confirmed human cases. We performed phylogenetic analysis on 39 full-genome H5N1 virus samples collected during outbreaks among poultry in 2015–2016 in West Java and compared them with recently published sequences from Indonesia. Phylogenetic analysis revealed that the hemagglutinin gene of all samples belonged to 2 genetic groups in clade 2.3.2.1c. We also observed these groups for the neuraminidase, nucleoprotein, polymerase, and polymerase basic 1 genes. Matrix, nonstructural protein, and polymerase basic 2 genes of some HPAI were most closely related to clade 2.1.3 instead of clade 2.3.2.1c, and a polymerase basic 2 gene was most closely related to Eurasian low pathogenicity avian influenza. Our results detected a total of 13 reassortment types among HPAI in Indonesia, mostly in backyard chickens in Indramayu.

Highly pathogenic avian influenza virus (HPAIV) H5N1 has been reported in Asia, including Indonesia since 2003. Although several risk factors related to the HPAIV outbreaks in poultry in Indonesia have been identified, little is known of the contact structure of farms of different poultry production types (backyard chickens, broilers, layers, and ducks). This study aims to quantify the contact rates associated with the movement of people, and movements of live birds and products and equipment that affect the risk of HPAIV H5N1 transmission between poultry farms in Indonesia. On 124 poultry farms in 6 districts in West Java, logbooks were distributed to record the movements of farmers/staff and visitors and their poultry contacts. Most movements in backyard chicken, commercial native chicken, broiler and duck farms were visits to and from other poultry farms, whilst in layer farms visits to and from poultry companies, visits to egg collection houses and visit from other poultry farms were most frequent. Over 75% of persons visiting backyard chicken and duck farms had previously visited other poultry farms on the same day. Visitors of backyard chicken farms had the highest average contact rate, either direct contact with poultry on other farms before the visits (1.35 contact/day) or contact during their visits in the farms (10.03 contact/day). These results suggest that backyard chicken farms are most at risk for transmission of HPAIV compared to farms of the other poultry production types. Since visits of farm-to-farm were high, backyard farms could also a potential source for HPAIV transmission to commercial poultry farms.

Knowledge of outbreaks and associated risk factors is helpful to improve control of the Highly Pathogenic Avian Influenza A(H5N1) virus (HPAI) in Indonesia. This study was conducted to detect outbreaks of HPAI H5N1 in endemically infected regions by enhanced passive surveillance, to describe the clinical manifestation of these outbreaks and identify associated risk factors. From November 2015 to November 2016, HPAI outbreak investigations were conducted in seven districts of West Java. In total 64 outbreaks were confirmed out of 75 reported suspicions and outbreak characteristics were recorded. The highest mortality was reported in backyard chickens (average 59%, CI95%: 49–69%). Dermal apoptosis and lesions (64%, CI95%: 52–76%) and respiratory signs (39%, CI95%: 27–51%) were the clinical signs observed overall most frequently, while neurological signs were most frequently observed in ducks (68%, CI95%: 47–90%). In comparison with 60 non-infected control farms, the rate of visitor contacts onto a farm was associated with the odds of HPAI infection. Moreover, duck farms had higher odds of being infected than backyard farms, and larger farms had lower odds than small farms. Results indicate that better external biosecurity is needed to reduce transmission of HPAI A(H5N1) in Indonesia.

Pet is an animal that lives to partially or completely dependent on humans for a particular purpose. Keep a pet as a companion animal, should pay attention to animal welfare. This study aims to gain insight about the relationship between education level and economic status of pet owners on knowledge andimplementation of the animal welfare principles. The data were collecting by direct interview or a face to face interview to the animal clinic or hospital visitors on site (accidental technique). The data were calculated by statistical methods chi square to examined the relationship or the impact of the two nominal variables and measure the strengthness of the relationships between variables with each other nominal variables.Data showed a significantly correlation between the economic status of n North Jakarta respondentsbehavior (p value is 0.040), with the economic status of the Central Jakarta respondents’ attitudes (p value is 0.020), level of education and attitude of South Jakarta respondents (p value is 0.000). There were no difference significantly between the level of education and economic status of the respondents’ knowledgewas observed in all study sites. However, based on current information and on site observation, North Jakarta and West Jakarta respondents were considered to be able to demonstrate more sufficient knowledge of the animal welfare principles.

Snakehead fish is currently used by the public as a health treatment because it has a higher content of albumin protein than others. The Research aimed was determined the protein quality and comparison levels in two species of snakehead fish, Channa striata and Channa micropeltes, derived from Bogor Regency. Protein concentration level of C. micropeltes and C. striata after drying for five minutes as much as 0.830 mg/mL and 0.803 mg/mL and these concentration were higher than dried for 10 and 15 minutes. Protein concentration levels of the two species were not significantly different. The molecular weight of albumin protein from both species were 65.56 kDa to dried for five minutes, 65.08 kDa to dried for 10 minutes, and 64.91 kDa to dried for 15 minutes. The Results concluded that there were no differences in protein levels in the fish meat and skin of the two species. Drying treatment affects the molecular weight of snakehead fish albumin protein.