Diagnosis in Periodontology: A Further Aid Through Microbiological Tests

D’Ercole, Simonetta; Catamo, Giovanni; Piccolomini, R

doi:10.1080/10408410701693317

Cited by 24 publications

(15 citation statements)

References 50 publications

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“…Nonetheless, population based antibiotic sensitivity tests for planktonic and biofilm growth of the bacteria, supplemented by clinical trials, should be conducted to support this claim. Whenever possible, individual patient culture and antibiotic susceptibility test should be performed as it outweighs any uniform protocol, even population based ones …”

Section: Discussionmentioning

confidence: 99%

Clinical and microbiological characterization of localized aggressive periodontitis: a cohort study

Oettinger‐Barak

Sela

Sprecher

et al. 2014

Australian Dental Journal

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Background: Localized aggressive periodontitis (LAgP) is an infectious periodontal disease which generally affects young people. Recent data suggest the involvement of different bacterial species in different populations. The causative bacterial species in Israel has never been identified despite a high prevalence of LAgP in this population. The objectives of this study were to characterize the bacterial microbiota of periodontal pockets within an Israeli LAgP population who were also clinically assessed. Methods: Twenty-one LAgP patients (test) and 12 chronic periodontitis patients (control) were examined. Bacterial samples were collected from periodontal pockets and analysed by both culture and polymerase chain reaction techniques. Mann-Whitney U test and chi-square test were used to compare results between the groups. Results: Higher levels of Parvimonas micra (>10

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Section: Discussionmentioning

confidence: 99%

Clinical and microbiological characterization of localized aggressive periodontitis: a cohort study

Oettinger‐Barak

Sela

Sprecher

et al. 2014

Australian Dental Journal

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“…The diagnostic and prognostic plaque or GCF markers for periodontitis include: 1) the presence of specific bacteria (including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans [previously Actinobacillus actinomycetemcomitans]); 49 2) bacterial products of metabolism (e.g., volatile sulfur compounds 50 or specific proteases; 51 3) biomarkers involved in the disease process but produced by the host (e.g., matrix metalloproteinases, 52 neutrophil elastase, and alkaline phosphatase); 53,54 4) biomarkers of tissue damage (e.g., hydroxyproline/collagen fragments); 53,55 and 5) other markers of the inflammatory process, such as prostaglandin E 2 and interleukin-1. 53 However, despite the complex nature of periodontal diseases, which involve a multifaceted immune and inflammatory reaction to a polymicrobial flora, and the interindividual variations in inflammatory responses, 56 such potential biomarkers are generally studied individually or, rarely, in small numbers.…”

Section: Diagnosis Of Periodontitis Based On Ir Spectra Of Gcfmentioning

confidence: 99%

An Update on Novel Non‐Invasive Approaches for Periodontal Diagnosis

Xiang

Sowa

Iacopino

et al. 2010

Journal of Periodontology

102

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READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en An Update on Novel Non-Invasive Approaches for Periodontal DiagnosisXiang, Xiaoming; Sowa, Michael G.; Iacopino , Anthony M.; Maev, Roman G.; Hewko, Mark D.; Man, Angela; Liu, Kan-Zhi Abstract For decades there has been an ongoing search for clinically acceptable methods for the accurate, non-invasive diagnosis and prognosis of periodontitis. There are several well-known inherent drawbacks with current clinical procedures. The purpose of this review is to summarize some of the newly emerging diagnostic approaches, namely, infrared spectroscopy, optical coherence tomography (OCT), and ultrasound. The history and attractive features of these new approaches are briefly illustrated, and the interesting and significant inventions related to dental applications are discussed. The particularly attractive aspects for the dental community are that some of these methods are totally non-invasive, do not impose any discomforts to the patients during the procedure, and require no tissue to be extracted. For instance, multiple inflammatory indices withdrawn from near infrared spectra have the potential to identify early signs of inflammation leading to tissue breakdown. Morphologically, some other non-invasive imaging modalities, such as OCT and ultrasound, could be employed to accurately measure probing depths and assess the status of periodontal attachment, the front-line of disease progression. Given that these methods reflect a completely different assessment of periodontal inflammation, if clinically validated, these methods could either replace traditional clinical examinations for the diagnosis of periodontitis or at least serve as attractive complementary diagnostic tools. However, the potential of these techniques should be interpreted more cautiously given the multifactorial character of periodontal disease. In addition to these novel tools in the field of periodontal inflammatory diseases, other alternative modalities like microbiologic and genetic approaches are only briefly mentioned in this review because they have been thoroughly discussed in other comprehensive reviews.Periodontitis is an endemic infectious disease of the tissues surrounding the teeth occurring in 50% of the population and may result in significant debilitation for about half of these persons. 1As the leading cause of edentulism, periodontitis may also have important implications for systemic health including an increased risk for cardiovascular disease and low birth weight/preterm delivery.2 According to a report by the World Health Organization, 3 severe periodontitis leading to tooth loss was found in 5% to 15% of most populations worldwide. Therefore, it is considered one of the most important global oral health burdens.Periodontitis is a multifactorial disease with microbial dental plaque as the in...

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“…In rare situations, such as atypical presentations or in ambiguous clinical circumstances, aid is sought from the laboratory. As such, culture-based identification has been the traditional Ôgold standard' for the detection of most bacterial and fungal infections (D'Ercole et al, 2008). The specimen is grown on a solid, semi-solid or liquid medium under aerobic, microaerophilic or anaerobic conditions.…”

Section: Culture-based Identification Of Microorganismsmentioning

confidence: 99%

“…In monitoring the disease progression or treatment outcomes of periodontal disease, the aforementioned traditional microbiological tests remain important (D'Ercole et al, 2008). However, when routine microbiological tests based on phenotypic characteristics alone do not yield sufficient information on the identification of a cultured isolate, molecular tests based on DNA probes might be of help.…”

Section: Identification Of Presumptively Novel Speciesmentioning

confidence: 99%

Exploring the oral bacterial flora: current status and future directions

et al. 2010

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Oral Diseases (2010) 16, 136–145 Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches.

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Diagnosis in Periodontology: A Further Aid Through Microbiological Tests

Cited by 24 publications

References 50 publications

Clinical and microbiological characterization of localized aggressive periodontitis: a cohort study

Clinical and microbiological characterization of localized aggressive periodontitis: a cohort study

An Update on Novel Non‐Invasive Approaches for Periodontal Diagnosis

Exploring the oral bacterial flora: current status and future directions

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