Diabetic Retinopathy and Laser Therapy in Rats: A Protein-Protein Interaction Network Analysis

Safaei, Akram; Tavirani, Mostafa Rezaei; Azodi, Mona Zamanian; Lashay, Alireza; Mohammadi, Seyed Farzad; Broumand, Mohamad Ghasemi; Peyvandi, Ali Asghar; Okhovatian, Farshad; Peyvandi, Hassan; Rostami‐Nejad, Mohammad

doi:10.15171/jlms.2017.s4

Cited by 18 publications

(17 citation statements)

References 71 publications

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“…7 Molecular investigations including genomics, proteomics, and metabolomics could be helpful to better understand the mechanisms by which the laser could be beneficial in different medical areas. [8][9][10] The effect of laser therapy and non-ionized radiation on gene expression has also been reported for Interleukin 6, vascular endothelial growth factor, MyoD and myogenin expressions in a tissue repairing process. 7,11 Other molecular levels such as proteome evaluations could provide key information regarding the molecular mechanisms related to the biostimulating effect of laser exposure on protein molecules.…”

Section: Introductionmentioning

confidence: 90%

Muscle Recovery Is Highlighted by IR Laser Therapy

et al. 2019

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Introduction: In sports medicine, laser application has been well-established for the recovery of muscles. The mechanisms by which benefits of this kind of therapy can be studied is molecular research approach. Protein-protein interaction network analysis as one of the important complementary studies of proteomics can accelerate this goal by the identification of novel contributing markers. Methods: By the use of Cytoscape V3.7.1 and its applications, a network of differential expressed proteins (DEPs) from IR laser treatment samples were constructed and analyzed. Six hub-bottlenecks were determined, 4 of which were from differentially expressed proteins. Results: ClueGO discovered 4 biological processes related to these hub-bottlenecks that their function could alter due to IR laser therapy. Conclusion: In fact, by the expression changes of hub-bottlenecks including the up-regulation of HSP90s, one of the prominent biological processes in muscle recovery could be activated. This process is called nitric oxide synthase (NOS) activation that could be proposed as one of the underlying mechanisms of IR laser treatments in muscle recovery.

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Section: Introductionmentioning

confidence: 90%

Muscle Recovery Is Highlighted by IR Laser Therapy

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“…[48][49][50] Using protein-protein interaction network analysis, researchers analyzed proteomics and large-scale data after laser irradiation in skin and diabetic retinopathy. 51,52 They reported that 43 biological terms (see Table 2) clustered in five groups were related to the critical genes which were dysregulated in the arm skin after CO 2 laser therapy. Ogita et al reported that the protein expression of human gingival fibroblasts which were exposed to lowlevel Er:YAG laser irradiation one day after exposure changed.…”

Section: Skin and Laser Therapymentioning

confidence: 99%

The Impact of Proteomic Investigations on the Development and Improvement of Skin Laser Therapy: A Review Article

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Introduction: Different molecular approaches have contributed to finding various responses of skin to external and internal tensions such as laser irradiation and many important mediators of skin disease have been identified through these approaches. However, different essential signals of skin biomarker pathways and proteins are partially detected or completely unknown. In the present study, the impact of proteomics on the evaluation of laser therapy for the treatment of skin diseases is investigated. Methods: The keywords of "Proteomics", "Laser therapy", "Skin", and "Skin disease" were searched in Google Scholar, Scopus and PubMed search engines. After screening, 53 documents were included in the study. Results: The global assessments revealed that different proteins in different signaling pathways of skin metabolism in terms of health or illness after laser therapy are expressed differentially. The results indicated that the application of proteomics is a useful method for promoting the results of laser interventions. Conclusion: This kind of research dealt with the practical proteomics of skin diseases and skin laser therapy.

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“…The elements that play a crucial role in the integrity of the constructed network (called central nodes) have been identified and their biological roles will investigate. [9][10][11] In the present study, gene expression changes in an arm skin biopsy after 7 days of laser therapy versus an untreated condition were investigated via network analysis to find significant events.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Cytokine-Mediated Signaling Pathway Dysregulation in Arm Skin After CO2 Laser Therapy

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Introduction: Laser therapy is known as an efficient approach in dermatology surgery. CO2 laser therapy is a gold standard treatment in skin surgery. This study aimed to evaluate the interferons change after CO2 laser surgery Methods: Significant differentially-expressed genes (DEGs) of arm skin after 7 days of treatment by the CO2 laser relative to the controls are downloaded from Gene Expression Omnibus (GEO) and are included in the protein-protein interaction network via a STRING database (an application of Cytoscape software). The central DEGs were identified and enriched via gene ontology by using Clue GO software. Results: A network including 78 DEGs and 100 neighbors was constructed and STAT1, MX1, ISG15, OAS1, IFIT1, IRF8, OASL, OAS2, and RSAD2 as hubs and STAT1, PTPRC, MX1, IRF8, ISG15, IL6, RORC, SAMSN1, and IFIT1 as bottlenecks were introduced. The cytokine-mediated signaling pathway, interferon gamma signaling, hepatitis C, interferon alpha/beta signaling, and the type I interferon signaling pathway were identified as 5 clusters of biological terms which are related to the central nodes. Conclusion: It can be concluded that the cytokine-mediated signaling pathway is the major pathway that is dysregulated after laser application in the treated skin.

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Diabetic Retinopathy and Laser Therapy in Rats: A Protein-Protein Interaction Network Analysis

Cited by 18 publications

References 71 publications

Muscle Recovery Is Highlighted by IR Laser Therapy

Muscle Recovery Is Highlighted by IR Laser Therapy

The Impact of Proteomic Investigations on the Development and Improvement of Skin Laser Therapy: A Review Article

Assessment of Cytokine-Mediated Signaling Pathway Dysregulation in Arm Skin After CO2 Laser Therapy

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