Diabetes and mass spectrometry

Lapolla, Annunziata; Fedele, Domenico; Traldi, Pietro

doi:10.1002/dmrr.189

Cited by 32 publications

(11 citation statements)

References 87 publications

(78 reference statements)

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“…In the second step, the glycated and non-glycated N-terminal hexapeptides of the obtained beta-chain are separated and quantified by HPLC and electrospray ionization mass spectrometry. Other mass spectrometrical methods are summarized by Lapolla et al [83].…”

Section: Post Mortem Analysesmentioning

confidence: 99%

Disorders of glucose metabolism–post mortem analyses in forensic cases: part I

2010

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In developed countries, diabetes is one of the ten most common causes of death. Post mortem diagnosis of glucose metabolism disorders can be difficult and vague because of the lack of characteristic morphological findings. Reviews of the literature are presented concerning biochemical problems in cases of unclear hyper- or hypoglycemia. After repetition of causes, frequency, and mortality of diabetic metabolism disorders, we give hints for the detection of diabetic ketoacidosis, hyperosmolar coma, insulinoma, and insulin- or oral diabetic-induced hypoglycemia. The first part discusses the analytes glucose and lactate, glycated proteins and oral antidiabetics, with special regard to their matrices post mortem, to reference concentrations, stability data and to analytic procedures that should be used in clinical or toxicological laboratories to detect diabetic metabolism disorders after death.

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Section: Post Mortem Analysesmentioning

confidence: 99%

Disorders of glucose metabolism–post mortem analyses in forensic cases: part I

2010

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“…This topic, which concerns the nonenzymatic attachment of carbohydrates to proteins, has been reviewed with emphasis on detection of glycated proteins by mass spectrometric techniques (Lapolla, Fedele, & Traldi, 2001b;Yeboah & Yaylayan, 2001). The sites glycosylated in human serum albumin are mainly surface-located lysine residues (Zoellner et al, 2001).…”

Section: Glycated Proteinsmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Harvey¹

2008

Mass Spectrometry Reviews

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This review is the second update of the original review on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates that was published in 1999. It covers fundamental aspects of the technique as applied to carbohydrates, fragmentation of carbohydrates, studies of specific carbohydrate types such as those from plant cell walls and those attached to proteins and lipids, studies of glycosyl-transferases and glycosidases, and studies where MALDI has been used to monitor products of chemical synthesis. Use of the technique shows a steady annual increase at the expense of older techniques such as FAB. There is an increasing emphasis on its use for examination of biological systems rather than on studies of fundamental aspects and method development and this is reflected by much of the work on applications appearing in tabular form.

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“…The more specific methods, based on HPLC, radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), subsequently used to study glycoxidation markers [17], have their limitations too, however, since they are unable to identify glycoxidation products structurally and they sometimes generate misleading results, as in the case of the first glycoxidation product identified—the FFI compound [18]. …”

Section: Markers Of Glycoxidationmentioning

confidence: 99%

Glyco-oxidation and cardiovascular complications in type 2 diabetes: a clinical update

2012

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Diabetes is associated with a greatly increased risk of cardiovascular disease (CVD), which cannot be explained only by known risk factors, such as smoking, hypertension, and atherogenic dyslipidemia, so other factors, such as advanced glycation end-products (AGEs) and oxidative stress, may be involved. In this frame, hyperglycemia and an increased oxidative stress (AGE formation, increased polyol and hexosamine pathway flux, and protein kinase C activation) lead to tissue damage, thus contributing to the onset of cardiovascular complications. Several studies have identified in various cell systems, such as monocytes/macrophages and endothelial cells, specific cellular receptors (RAGE) that bind AGE proteins. The binding of AGEs on RAGE induces the production of cytokines and intracellular oxidative stress, thus leading to vascular damage. Soluble RAGE levels have been identified as hypothetical markers of CVD, but, in this regard, there are sparse and conflicting data in the literature. The purpose of this review was to examine all the available information on this issue with a view to clarifying or at least highlighting the points that are still weak, especially from the point of clinical view.

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Diabetes and mass spectrometry

Cited by 32 publications

References 87 publications

Disorders of glucose metabolism–post mortem analyses in forensic cases: part I

Disorders of glucose metabolism–post mortem analyses in forensic cases: part I

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Glyco-oxidation and cardiovascular complications in type 2 diabetes: a clinical update

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