Developmental changes of nutrient digestion in young lambs are influenced by weaning and associated with intestinal microbiota

Li, Chong; Wang, Guoxiu; Zhang, Qian; Huang, Yongliang; Li, Fadi; Wang, Weimin

doi:10.1080/10495398.2022.2025817

Cited by 5 publications

(6 citation statements)

References 69 publications

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“…The apparent digestibility of DM, CP, starch, fat, NDF, and ADF significantly decreased by 21.77%, 23.15%, 23.62%, 19.38%, and 45.24%, respectively, from 7 to 35 days of age. This is consistent with the change rule of nutrient digestibility [ 12 ], which proposes that increased fiber results in a reduction of apparent nutrient digestibility [ 41 , 42 ]. Furthermore, we analyzed the correlation between nutrient digestibility and the fecal microflora, and the correlation between fecal nutrient contents and the fecal microflora, respectively, to explore the interaction and causality between host nutrient digestion and the microflora.…”

Section: Discussionsupporting

confidence: 88%

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Periodical Changes of Feces Microbiota and Its Relationship with Nutrient Digestibility in Early Lambs

Huang

Wang

et al. 2022

Animals

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Early mammal gut microorganism colonization affects host health, the feed conversion rate, and production performance. Herein, we explored how fecal microbiota develops and the interactions between microorganisms and nutrients. The lambs were separated from ewes at 7 days old, artificial feeding with milk replacer completely replaced lactation, and the starter diet was added. At 21 days old, the lambs were fed with complete starter and milk replacer was stopped. At day 7, 21, 35, and 49 after birth, fecal samples were collected. Then 16S rRNA gene sequencing in the fecal samples revealed that the alpha diversity increased significantly with age. Principal coordinates analysis showed clear clustering by age (p < 0.05). At the genus level, the relative abundance of 8 genera declined, 12 genera increased (p < 0.1), and 4 genera changed dramatically with age (p < 0.05). The apparent digestibility of dry matter, protein, fat, neutral detergent fiber, and acid detergent fiber significantly decreased by 21.77%, 23.15%, 23.62%, 19.38%, and 45.24%, respectively, from 7 to 35 days of age (p < 0.05), but not thereafter (p > 0.05). Fecal nutrient contents affected the abundance of bacterial genera (p < 0.05). Enterobacteriaceae_unclassified, Clostridium XlVb, Bifidobacterium, and other genera had no relationship with the fecal nutrient content; however, they were closely related to nutrient intake and digestibility, possibly promoting nutrient digestion. Our results suggested that nutrient digestion of young lambs changed rapidly, which was closely related to intestinal microbial colonization.

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Section: Discussionsupporting

confidence: 88%

“…The development of the nutrient digestibility of lambs is very important for their whole life. The early postnatal and weaning stages are key for lamb digestive tract development [ 12 ]. However, the interaction and causal links between the gastrointestinal microflora and the digestibility of the host are still uncertain.…”

Section: Introductionmentioning

confidence: 99%

Periodical Changes of Feces Microbiota and Its Relationship with Nutrient Digestibility in Early Lambs

Huang

Wang

et al. 2022

Animals

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“…Up to 3 weeks old, the growth performance of lambs was significantly improved by the higher level of MR feeding, although the low-level feeding group had a higher starter feed intake. The intake of solid feed in lambs before 3 weeks old is low, and the digestion and utilization efficiency of plant-based feed was much lower than that of MR (Li et al, 2022). Thus, at this stage, milk or MR provides most of the nutrients needed for growth and development, and the better bioavailability of protein and energy along with minerals, enzymes, and growth factors results in increased weight gains (Lee et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Stoll DNA kit (Omega Bio-Tek, Winooski, VT, United States). The sequencing process was the same as that detailed our previous study ( Li et al, 2022 ). DNA quality and quantity were assessed using a NanoDrop 2000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, United States).…”

Section: Methodsmentioning

confidence: 99%

Effects of milk replacer feeding level on growth performance, rumen development and the ruminal bacterial community in lambs

et al. 2023

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Feeding with a suitable level of milk replacer (MR) can improve the survival rate and stimulate the growth potential of early lambs. However, feeding excessive MR might be detrimental to rumen development and microbial colonization. Herein, we investigated the effects of feeding different levels of MR on rumen digestive function and ruminal microorganisms. Fourteen healthy male Hu lambs with similar birth weights and detailed pedigree records were divided into two groups to receive low (2% of average body weight per day) and high (4% of average body weight per day) levels of MR. We analyzed the effects of the MR feeding level on growth performance, fiber degradation rates, rumen fermentation parameters, enzyme activities and rumen histomorphology. We found that feeding with a high level of MR improved the average daily gain of early lambs, but decreased the starter intake, rumen weight and papillae length. We also analyzed the effects of the MR feeding level on the rumen microbiota using 16S-rRNA amplicon sequencing data. The results showed that high a MR feeding level increased the rumen microbial diversity but decreased the abundance of many carbohydrate degrading bacteria. Several bacterial genera with significant differences correlated positively with rumen cellulase activity and the acid detergent fiber degradation rate. Our results suggested that a high level of MR could improve the growth performance of early lambs in the short term; however, in the long term, it would be detrimental to rumen development and have adverse effects on the adaptation process of the microbiota to solid feed.

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“…The results showed that the acetic acid and total acid contents of Tianhua mutton sheep were higher than those of Gansu alpine fine wool sheep. It has been reported that excessive intake of roughage with a high fiber content will increase the acetic acid content in the rumen [30,31]. A large amount of acetic acid produced by rumen fermentation in sheep is oxidized through the tricarboxylic acid cycle for energy supply or used for fatty acid synthesis to maintain energy intake in winter [32,33].…”

Section: Differences In Volatile Fatty Acids In the Rumen Contents Of...mentioning

confidence: 99%

Rumen Development of Tianhua Mutton Sheep Was Better than That of Gansu Alpine Fine Wool Sheep under Grazing Conditions

Li,

Liu,

Duan

et al. 2024

Animals

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The purpose of this experiment was to investigate the differences in rumen tissue morphology, volatile fatty acid content, and rumen microflora between Tianhua mutton sheep and Gansu alpine fine wool sheep under the same grazing conditions. Twelve 30-day-old lambs were randomly selected from two different flocks in Duolong Village and grazed together for a period of 150 days. The rumen tissue was fixed with 4% paraformaldehyde and brought back to the laboratory for H&E staining, the volatile fatty acid content of the rumen contents was detected by gas chromatography, and the rumen flora structure was sequenced by full-length sequencing of the bacterial 16S rRNA gene using the PacBio sequencing platform. The acetic acid and total acid contents of the rumen contents of Tianhua mutton sheep were significantly higher than those of Gansu alpine fine wool sheep (p < 0.05). The rumen papillae height of Tianhua mutton sheep was significantly higher than that of Gansu alpine fine wool sheep (p < 0.05). The diversity and richness of the rumen flora of Tianhua mutton sheep were higher than those of Gansu alpine fine wool sheep, and Beta analysis showed that the microflora structure of the two fine wool sheep was significantly different. At the phylum level, Firmicutes and Bacteroidetes dominated the rumen flora of Tianhua mutton sheep and Gansu alpine fine wool sheep. At the genus level, the dominant strains were Christensenellaceae_R_7_group and Rikenellaceae_RC9_gut_group. LEfSe analysis showed that Prevotella was a highly abundant differential species in Tianhua mutton sheep and lachnospiraccac was a highly abundant differential species in Gansu alpine fine wool sheep. Finally, both the KEGG and COG databases showed that the enrichment of biometabolic pathways, such as replication and repair and translation, were significantly higher in Tianhua mutton sheep than in Gansu alpine fine wool sheep (p < 0.05). In general, there were some similarities between Tianhua mutton sheep and Gansu alpine fine wool sheep in the rumen tissue morphology, rumen fermentation ability, and rumen flora structure. However, Tianhua mutton sheep had a better performance in the rumen acetic acid content, rumen papillae height, and beneficial bacteria content. These differences may be one of the reasons why Tianhua mutton sheep are more suitable for growing in alpine pastoral areas than Gansu alpine fine wool sheep.

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Developmental changes of nutrient digestion in young lambs are influenced by weaning and associated with intestinal microbiota

Cited by 5 publications

References 69 publications

Periodical Changes of Feces Microbiota and Its Relationship with Nutrient Digestibility in Early Lambs

Periodical Changes of Feces Microbiota and Its Relationship with Nutrient Digestibility in Early Lambs

Effects of milk replacer feeding level on growth performance, rumen development and the ruminal bacterial community in lambs

Rumen Development of Tianhua Mutton Sheep Was Better than That of Gansu Alpine Fine Wool Sheep under Grazing Conditions

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