Developmental Changes of Lymphoid Tissue in the Harderian Gland of Laying Hens

Bejdić, Pamela; Avdić, Rizah; Amidžić, Ljiljana; Ćutahija, Velida; Tandir, Faruk; Hadžiomerović, Nedžad

doi:10.14432/j.macvetrev.2014.02.009

Cited by 10 publications

(11 citation statements)

References 13 publications

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“…In our previous studies we observed the enlarged lymphoid tissue in the harderian gland of vaccinated chickens compared to the non-vaccinated ones . Our results confi rm the data, stating that lymphoid nodules of the harderian gland are not observed in the chickens up to the age of 40 days (Bejdic P et al, 2014.…”

Section: Discussionsupporting

confidence: 91%

Immune response of the harderian gland in chickens to infectious bronchitis coronavirus

et al. 2021

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Aim. To determine the difference in immune responses of the harderian gland in clinically healthy chickens and the ones with infectious bronchitis based on the content, localization and morphometric estimation of the surface markers of Т- and В-lymphocytes and to determine the differentiation index as an indicator of assessing body defenses. Methods. Histological, immunohistochemical, optical, morphometric and statistical. Results. The histological study of the harderian gland of chickens with infectious bronchitis determined the swelling and proliferation of the connective tissue as well as infiltration of secretory lobules by lymphoid cells. It was found that the immunity of chickens with infectious bronchitis, in which the harderian gland plays a relevant role, depends considerably on the differentiation index of immunocompetent cells. There was a reliable 1.77- and 1.36-fold decrease in this indicator for 40- and 90-day-old chickens, respectively, in case of nephroso-nephritic form of infectious bronchitis which demonstrated a weaker function of the defense cells of this organ. According to the cytomorphometric analysis, the number of cells, expressing CD4+, CD8+, CD20+, CD45RA+ markers in the harderian gland of sick 20-, 40-, and 90-day-old chickens with respiratory and nephroso-nephritic forms of infectious bronchitis was reliably (P < 0.05) increasing compared to the clinically healthy chickens. For instance, the number of mature В-lymphocytes increased in sick 20-day-old chickens – 2.44 times, 40-day-old chickens – 1.88 times, and 90-day-old ones – 2.62 times compared to clinically healthy chickens. Conclusions. The data were obtained about the changes in quantitative and qualitative composition of lymphocytes with surface markers CD4+, CD8+, CD20+, CD45RA+ in the harderian gland of chickens with infectious bronchitis. Our results will supplement current knowledge about the feasibility of immunohistochemical methods in the diagnostics of avian infectious bronchitis.

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Section: Discussionsupporting

confidence: 91%

Immune response of the harderian gland in chickens to infectious bronchitis coronavirus

et al. 2021

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“…The findings of the present study regarding presence of intense PAS positive activity in the plasma cells and Russell bodies in the interstitial connective tissue of the Harderian gland at 17 and 24 days of age ( Fig 5) coincides with the observations reported by W ight et al (1971) in domestic fowl and Bejdic et al (2014) in laying hens.…”

Section: Histochemical Changes During Development Of Harderian Gland supporting

confidence: 93%

Histochemical changes during development of Harderian gland in chicken

Rana¹,

Banubakode²,

Nandeshwar³

et al. 2019

IJAR

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The present study was aimed to examine histochemical changes in the Harderian gland of pre and post hatched chicks from 11 day of incubation to 24 days of age. In pre hatched groups, the gland was in the developing stage. There was no well defined parenchyma, septa and capsule in early embryonic stage. Histochemical observation revealed that weak or negligible activity of PAS, AB/PAS and Sudan black B was seen in epithelial lining of Harderian gland on 18th and 20th day of incubation. The gland was compound tubule-acinar type at 20th day of incubation, consisted of capsule, parenchyma and stroma. PAS activity was seen in the capsule, septa, epithelial lining of acini and tubule and interstitial connective tissue cells. PAS activity was aggregated towards the apical part of the tubular epithelium as well as at the free luminal surface epithelium of the acini. PAS activity increases with the development of connective tissue component of Harderian gland with the advancement of age. The Harderian gland was predominantly mucus and lipid secreting gland. All epithelial cells of the glandular unit of Harderian gland contain both neutral and acidic mucin. Intense AB-positive activity was seen at the apex of tubule with the advancement of age. Sudan black B activity was recorded in the cytoplasm of epithelial lining of tubule and acini of the Harderian gland and their presence increases with age.

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“…The fowl HG has a large agedependent populations of plasma cells and is capable to produce antibodies both to systemically and locally applied antigens (Burns, 1992). The plasma cells of HG produce or participate in the transmission of four classes of immunoglobulins: IgA, IgG, IgM and IgY (Ohshima & Hiramatsu, 2002;Bejdic et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants

Klećkowska‐Nawrot

Goździewska‐Harłajczuk

Kowalczyk

et al. 2016

Rev. Bras. Cienc. Avic.

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Harderian gland, histology, histochemistry, common pheasant, hybrid of Italian amber and common pheasant. ABStRACtThe aim of the present study was to demonstrate the histological and histochemical structure of the Harderian gland in wild and hybrid of wild and domestic birds. The samples were stained with haematoxylin-eosin, methyl green-pyronin Y, periodic acid-Schiff, alcian blue pH 2.5, aldehyde fuchsin and Hale's dialyzed iron staining's. In both species, the glands had multilobar tubuloacinar structure type I. The Harderian gland was located in the orbit near the inter-orbital septum, between the medial rectus muscle, the pyramidal muscle of the third eyelid, and the ventral oblique muscle. In the common pheasant, the gland was wider in the proximal and distal part. The common pheasant had more elongated lobes of the Harderian gland than in the hybrid. In the common pheasant, the glandular cells presented darkly-stained serous secretion and lightly-stained mucous secretion. In the hybrid, the glandular cells presented seromucous secretion. The central lobar space, interacinar space, and apical parts of the acini of the Harderian glands were filled with many lymphocytes and plasma cells, particularly in the common pheasant, where centers of all large lobes were abundantly filled with plasma cells. The plasma cells dominated in common pheasant's Harderian gland, while in the hybrid, lymphocytes and plasma cells were present at similar quantities. The cells positive for periodic acid of Schiff staining were dominant in hybrid. Periodic acid-Schiff, Hale's dialyzed iron and alcian blue pH 2.5 stainings demonstrated acid-carboxylated mucopolysaccharides in the glandular cells cytoplasm of the examined birds.

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Developmental Changes of Lymphoid Tissue in the Harderian Gland of Laying Hens

Cited by 10 publications

References 13 publications

Immune response of the harderian gland in chickens to infectious bronchitis coronavirus

Immune response of the harderian gland in chickens to infectious bronchitis coronavirus

Histochemical changes during development of Harderian gland in chicken

Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants

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