Development, Structure, and Keratin Expression in <i>C57BL/6J</i> Mouse Eccrine Glands

Taylor, Douglas K; Bubier, Jason A.; Silva, Kathleen A.; Sundberg, John P.

doi:10.1177/0300985811430511

Cited by 29 publications

(34 citation statements)

References 38 publications

(95 reference statements)

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“…From day 21, the inner layer of the spheroid-like structures successively loses K5 and K14 expression, and the outer layer expresses alpha-SMA, suggesting myoepithelial differentiation. In this study, we have not examined the specific ductal markers of K1b, K10, and K11, but the loss of K5, K14, and K15 expression might reflect ductal differentiation (Moll and Moll 1992;Taylor et al 2012;Langbein et al 2013). These results show the complex development processes of 3D-reconstituted eccrine sweat glands and their cellular differentiation.…”

Section: Discussionmentioning

confidence: 87%

Changes in keratins and alpha-smooth muscle actin during three-dimensional reconstitution of eccrine sweat glands

Zhang

et al. 2016

Cell Tissue Res

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We have examined the changes of keratins and alpha-SMA at various time points in order to investigate the development and differentiation of eccrine sweat gland cells during the course of three-dimensional (3D) reconstitution. Mixtures of eccrine sweat gland cells and Matrigel were injected subcutaneously into the inguinal regions of nude mice. At 1, 2, 4, 6, 8, 14, 21, 28, 35, and 42 days post-implantation, Matrigel plugs were removed and immunostained. We found that during 3D reconstitution, keratin and alpha-SMA expression changed in a time-dependent manner. At day 1, all cells stained positively for keratin isoforms K5, K14, and K15, with the staining intensity of K15 being weak and K5 and K14 being strong, but none of the cells displayed K7, K8, or alpha-SMA. As time progressed, spheroid-like structures formed with the inner layer acquiring K7 and K8, but losing K5 and K14 expression, and the outer layer acquiring alpha-SMA expression, but losing K15 expression. K8 expression was first noted at day 14, and K7 and alpha-SMA at day 21. The loss of K15 expression was first noted at day 14, K14 at day 21, and K5 at day 28. At 28, 35, and 42 days, the spheroid-like structures could be distinguished, by immunohistochemistry, as having secretory coil-like and coiled duct-like structures. We conclude that the changes in expression of keratins and alpha-SMA in 3D-reconstituted eccrine sweat glands are similar to those of native eccrine sweat glands, indicating that the 3D reconstitution of sweat glands provides an excellent model for studying the development, cytodifferentiation, and regulation of eccrine sweat glands.

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Section: Discussionmentioning

confidence: 87%

Changes in keratins and alpha-smooth muscle actin during three-dimensional reconstitution of eccrine sweat glands

Zhang

et al. 2016

Cell Tissue Res

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“…For example, many vertebrates display regional colour variation, with dorsal hair being lighter than hair found in the ventral surface . In mammals, mammary and eccrine glands tend to be restricted to the ventral surface of the body and to the palmoplantar skin of the feet, respectively, and in birds, there are regions devoid of feathers and marked differences in the scales of the dorsal and ventral surfaces of the feet . A number of studies using mouse and chicken as models have elucidated some of the genes controlling these and other local spatial differences found in the skin, revealing key insights into the molecular mechanisms by which regionalization is established during embryogenesis (reviewed in ref ).…”

Section: Background: Skin Biologymentioning

confidence: 99%

Periodic patterns in Rodentia: Development and evolution

Johnson

Barsh

Mallarino

2019

Experimental Dermatology

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Mammalian periodic pigment patterns, such as spots and stripes, have long interested mathematicians and biologists because they arise from non-random developmental processes that are programmed to be spatially constrained, and can therefore be used as a model to understand how organized morphological structures develop. Despite such interest, the developmental and molecular processes underlying their formation remain poorly understood. Here, we argue that Arvicanthines, a clade of African rodents that naturally evolved a remarkable array of coat patterns, represent a tractable model system in which to dissect the mechanistic basis of pigment pattern formation. Indeed, we review recent insights into the process of stripe formation that were obtained using an Arvicanthine species, the African striped mouse (Rhabdomys pumilio), and discuss how these rodents can be used to probe deeply into our understanding of the factors that specify and implement positional information in the skin. By combining naturally evolved pigment pattern variation in rodents with classic and novel experimental approaches, we can substantially advance our understanding of the processes by which spatial patterns of cell differentiation are established during embryogenesis, a fundamental question in developmental biology.

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“…The number of obstructed acrosyringia was pronouncedly higher in Flg mutant mice compared with wild-type mice (Figure 2a and b). To evaluate the nature of the obstructed material seen in the sections, we performed immunofluorescence staining using an antibody against mouse keratin 6 that is expressed only in the sweat ducts and not in the eccrine glands (Taylor et al, 2012). Interestingly, the amorphous substances were stained positively with the anti-mouse keratin 6 ( Figure 2c).…”

Section: To the Editormentioning

confidence: 99%