Development of vitamin C irrigation saline to promote graft healing in anterior cruciate ligament reconstruction

Fu, Sai‐Chuen; Cheng, Wai Hang; Cheuk, Yau-Chuk; Mok, Tsui Yu; Rolf, Christer; Yung, Patrick Shu‐Hang; Chan, Kai Ming

doi:10.1016/j.jot.2013.06.001

Cited by 18 publications

(33 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Positive treatment effects were observed at 6 weeks post‐operation (after cessation of GHK‐Cu treatment), while no significant improvement was observed at 12 weeks post‐operation. The second limitation of the current study was the discrepancies between rat and human models which may restrict extrapolation of the findings from the experimental model to clinical application, which has been discussed in previous reports . The third limitation was the use of LII when evaluating functional recovery after ACLR in rats, which has previously been discussed .…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Tripeptide-copper complex GHK-Cu (II) transiently improved healing outcome in a rat model of ACL reconstruction

Cheuk

Chiu

et al. 2015

J. Orthop. Res.

Self Cite

View full text Add to dashboard Cite

After anterior cruciate ligament reconstruction (ACLR), the biological healing of the graft is a rate-limiting step which can contribute to graft failure. The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu(II) (GHK-Cu) is a well-known activator of tissue remodeling. We investigated whether GHK-Cu can improve graft healing following ACLR. Seventy-two rats underwent unilateral ACLR were randomized to saline, 0.3 or 3 mg/ml GHK-Cu groups (n ¼ 24). Post-operational intra-articular injections were given from week 2, once a week, for 4 weeks. Gait analysis was performed pre-injury and at harvesting time. At 6 or 12 weeks post-operation, knee specimens were harvested for knee laxity test, graft pull-out test, and histology. At 6 weeks post-ACLR, GHK-Cu groups resulted in a smaller side-to-side difference in knee laxity as compared to the saline group (p ¼ 0.009), but there was no significant difference at 12 weeks post-operation. The graft complex in the 0.3 mg/ml GHK-Cu group had higher stiffness than saline group at 6 weeks postoperation (p ¼ 0.026), but there was no significant difference in ultimate load, gait parameters, and histological scores among treatment groups. All grafts failed mid-substance during pull-out test. Intra-articular supplementation with a bioactive small molecule GHK-Cu improved graft healing following ACLR in rat, but the beneficial effects could not last as treatment discontinued. ß

show abstract

Section: Discussionmentioning

confidence: 91%

“…and Y.C.C.) blinded to treatments according to a developed scoring system, based on the extent of matrix degeneration of the tendon graft, and the healing responses in the graft–tunnel interface. Image analysis of cell density in graft mid‐substance was performed using Image‐Pro Premier 9.1 (Media Cybernetics, Bethesda, MD).…”

Section: Methodsmentioning

confidence: 99%

Tripeptide-copper complex GHK-Cu (II) transiently improved healing outcome in a rat model of ACL reconstruction

Cheuk

Chiu

et al. 2015

J. Orthop. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…To our knowledge, there are relevant studies published in new journals that are not included in the 3 search databases. 17 Because we identified good-quality studies with an arbitrary cutoff score (≥5), it is likely that we may have missed some meaningful findings during the integration of clinical/experimental evidence. If we lowered the cutoff scores to 4, 12 studies would be further included.…”

Section: Discussionmentioning

confidence: 99%

Systematic Review of Biological Modulation of Healing in Anterior Cruciate Ligament Reconstruction

Cheuk

Yung

et al. 2014

Orthopaedic Journal of Sports Medicine

Self Cite

View full text Add to dashboard Cite

Background:Whether biological modulation is effective to promote healing in anterior cruciate ligament (ACL) reconstruction remains unclear.Purpose:To perform a systematic review of both clinical and experimental evidence of preclinical animal studies on biological modulation to promote healing in ACL reconstruction.Study Design:Systematic review; Level of evidence, 2.Methods:A systematic search was performed using the PubMed, Ovid, and Scopus search engines. Inclusion criteria were clinical and animal studies involving subjects with ACL injury with the use of biological modulation to promote healing outcomes. Methodological quality of clinical studies was evaluated using the Critical Appraisal Skill Programme (CASP) appraisal tool, and animal studies were evaluated by a scoring system based on a published checklist of good animal studies.Results:Ten clinical studies and 50 animal studies were included. Twenty-five included studies were regarded as good quality, with a methodological score ≥5. These studies suggested that transforming growth factor–beta (TGF-β), mesenchymal stem cells, osteogenic factors, and modalities that reduce local inflammation may be beneficial to promote graft healing in ACL reconstruction.Conclusion:This systematic review suggests that biological modulation is able to promote healing on top of surgical treatment for ACL injuries. This treatment strategy chiefly works through promotion of healing at the tunnel-graft interface, but the integrity of the intra-articular midsubstance of the graft would be another target for biological modulation.

show abstract

“…In 2013, Fu et al [150] used isotonic irrigation of ascorbic acid derivative during grafting of the anterior tendon of the knee joint. Significant reduction of the inflammatory response in the surgical site due to lack of toxicity, irritation, watery consistency and potent anti-oxidant effect.…”

Section: Vitamin C and Bone Formationmentioning

confidence: 99%

Vitamin C

Yussif¹

2019

Vitamin C - An Update on Current Uses and Functions

View full text Add to dashboard Cite

Vitamin C or ascorbic acid is one of the most common and essential vitamins. Due to its protective role, the supplementation of vitamin C becomes a must especially during the higher pollution levels. A day after day, scientists and researchers discover new functions for vitamin C. It was and still one of the cheapest treatment modalities that could preserve and protect human beings from infections, toxification, autoimmune diseases and cancer development. The role of vitamin C in providing better esthetics exhibits great importance. Its role as anti-aging agent preserves skin color and texture. Although it is not naturally synthesized in our bodies, our food is entirely rich of it.

show abstract

Development of vitamin C irrigation saline to promote graft healing in anterior cruciate ligament reconstruction

Cited by 18 publications

References 32 publications

Tripeptide-copper complex GHK-Cu (II) transiently improved healing outcome in a rat model of ACL reconstruction

Tripeptide-copper complex GHK-Cu (II) transiently improved healing outcome in a rat model of ACL reconstruction

Systematic Review of Biological Modulation of Healing in Anterior Cruciate Ligament Reconstruction

Vitamin C

Contact Info

Product

Resources

About