Development of surface plasmon resonance (SPR) biosensors for use in the diagnostics of malignant and infectious diseases

Abstract

Surface plasmon resonance (SPR) has become an important optical biosensing technology due to its real-time, label-free, and noninvasive nature. These techniques allow for rapid and ultra-sensitive detection of biological analytes, with applications in medical diagnostics, environmental monitoring, and agriculture. SPR is widely used in the detection of biomolecular interactions, and improvements are required for both sensitivity and in vivo uses for practical applications. In this study, we developed an SPR biosensor to provide a highly sensitive and specific approach to early-stage detection of viral and malignant diseases, such as cancer tumors, for which biomarker detection is very important. A cancer cell line (HeLa cells) with biomarker Rodamine 6G was experimentally analyzed in vitro with our constructed SPR biosensor. It was observed that the biosensor can offer a potentially powerful solution for tumor screening with dominant angular shift. The angular shift for both regents is dominant with a time curve at a wavelength of 632.8 nm of a He–Ne laser. We have successfully captured and detected a biomarker in vitro for cancer diagnostics using the developed instrument.

Point-of-care diagnostics for infectious diseases: From methods to devices

“…SPR, which measures the changes in the refractive index caused by molecular interactions on the metal surface via a surface plasmon wave, has become one of the most powerful detection methods for the POC diagnostic applications due to its merits of real-time, label-free, high cost-effective, noninvasive nature, excellent reutilization performance and outstanding reproducibility [229] , [230] , [231] , [232] , [233] . And as a result, various SPR-based biosensors have been developed for POC detection of infectious diseases [82] , [168] , [234] , [235] , [236] , [237] , [238] , among which some have already been adapted into POC devices [239] , [240] , [241] .…”

Fiber-Optic Localized Surface Plasmon Resonance Sensors Based on Nanomaterials

“…Among the various signal detection and information acquisition modalities in biological sensors, optical approaches have been actively studied as they can offer high specificity, high sensitivity, feasibility of direct/real-time measurements, and scope for miniaturization [ 70 , 71 ]. In particular, SPR-based optical biological sensors have been researched [ 72 , 73 , 74 , 75 ] and commercialized in several enterprises [ 76 , 77 , 78 ] as they can measure fine variations in refractive indices with high sensitivity. Studies of biological sensors using nanomaterial-based LSPR to further improve sensitivity have also been actively pursued [ 34 , 79 , 80 , 81 , 82 , 83 ].…”

The Principle of Nanomaterials Based Surface Plasmon Resonance Biosensors and Its Potential for Dopamine Detection

“…It was demonstrated that SPR sensor has the ability to detect pregnancy associated plasma protein A2, which is a metalloproteinase that plays multiple roles in fetal development and postnatal growth [35]. Several studies were reported on using SPR sensor as a new strategy to detect influenza nucleoprotein [36], avian influenza A H7N9 virus [37], maize chlorotic mottle virus [38], herpes simplex virus (HSV) [39], cancer cell line (HeLa cells) with biomarker Rhodamine 6G related to cancer tumors [40], nonstructural protein 1 of Zika virus [41], non-human pathogen feline calicivirus (FCV) [42], and dengue virus (DENV) [43][44][45][46].…”