Development of SSR Markers for Identification of Korean Ginseng (Panax ginseng C. A. Meyer) Cultivars

Bang, Kyong-Hwan; Chung, Jong‐Wook; Kim, Young-Chang; Lee, Jei-Wan; Jo, Ick-Hyun; Seo, A-Yeon; Kim, Ok-Tae; Hyun, Dong-Yun; Kim, Dong‐Hwi; Cha, Seon‐Woo

doi:10.7783/kjmcs.2011.19.3.185

Cited by 17 publications

(10 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Besides, many researchers have tried to identify ginseng species and varieties using SSR markers Ma et al 2007;Nguyen et al 2010;Bang et al 2011aBang et al , 2011b. SSR markers are important for several types of research, including the assessment of genetic diversity, development of genetic maps, comparative genomics, marker-assisted selection, and other plant breeding fields.…”

Section: Introductionmentioning

confidence: 99%

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

Um¹,

Mitsugi²,

Kim³

et al. 2016

Plant Breed. Biotech

Self Cite

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2016 (February) ABSTRACT Panax ginseng has been one of the most important herbal medicines used in Eastern Asia. Recently, various molecular markers have been developed to authenticate Panax species, but these markers cannot differentiate the exact varieties or variants of Korean ginseng cultivars. In this study, six cultivars of Korean ginseng (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng were differentiated by simple sequence repeat (SSR) marker development. Specific primer sets were designed for the 54 candidate sequences containing SSRs that were predicted. Finally, eight polymorphic SSR loci were developed. DNA fragment analysis was performed using fluorescence-labelled primers for the amplicons. Reproducibility tests were carried out using multiple samples of Korean ginseng cultivars and Panax species. Eight primer sets (PgSSR07, PgSSR08, PgSSR09, PgSSR17, PgSSR37, PgSSR40, PgSSR51, and PgSSR53) showing polymorphism were used for phylogenetic relationship analysis. Consequently, six Korean ginseng cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng could be identified using the combination of SSR markers discovered.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

Um¹,

Mitsugi²,

Kim³

et al. 2016

Plant Breed. Biotech

Self Cite

View full text Add to dashboard Cite

show abstract

“…With respect to GD and PIC, which represent genetic diversity, both were lowest in WCGSSR7 (0.36 and 0.33, respectively) and highest in WCGSSR8 (0.77 and 0.74, respectively) while their means were 0.588 and 0.548, respectively (Table 3). Bang et al (2011a), found that the mean N A found in P. ginseng cultivars using SSR markers is 2.6 and the mean PIC value is 0.480. Moreover, it has been reported that domestic and foreign ginseng accessions and collected species have a mean N A of 4.3 and mean genetic diversity of 0.553 (Bang et al 2011b).…”

Section: Ssr Polymorphismmentioning

confidence: 99%

“…The RAPD (random amplification of polymorphic DNA) method has been used to analyze gene diversity among P. quinquefolius (Bai et al 1997;Lim et al 2007), identify P. quinquefolius and P. ginseng (Boehm et al 1999;Shim et al 2003), and analyze the genetic relationships of native P. ginseng (Seo et al 2003). Analyses using SSR markers, which offer the advantage of being codominant markers with excellent reproducibility, have been used in the identification of major P. ginseng cultivars and analysis of genetic diversity among various domestic and foreign ginseng cultivars (Bang et al 2011a;Bang et al 2011b;Bang et al 2013;Hamada et al 1982;Tautz and Renz, 1984) while sequence-related amplified polymorphism (SRAP) analysis has been used to study genetic diversity in Chinese ginseng cultivated in China (Xu et al 2010). Analyses of genetic diversity and the relationships among crops increase the efficiency of breeding cultivars by expanding genetic mutations based on accurate genetic information and improvements in breeding (Tatineni et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of Chinese ginseng accessions using SSR markers

Park

Hong

et al. 2017

J Plant Biotechnol

Self Cite

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract The need to preserve and use plant genetic resources is widely recognized, and the prospect of dwindling plant genetic diversity, coupled with increased demands on these resources, has made them a topic of global discussion. In the present study, the genetic diversity and population structure of 73 ginseng accessions collected from six regions in China were analyzed using eight simple sequence repeat (SSR) markers. Major allele frequencies ranged between 0.380 .78, with a mean allele frequency value of 0.571. The number of alleles discovered ranged from 3 to 10 per accession, with a mean number of 7; 56 alleles were discovered in total. Gene diversity (GD) and polymorphic information content (PIC) values were similar to each other, and they ranged from 0.36 0.77 (mean 0.588) and 0.33~0.74 (mean 0.548), respectively. Accessions were divided into three clusters based on their phylogenetic relationships and genetic similarities, and although the populations were similar, they were not classified according to the region. Regional genetic diversity was also similar, with slight differences observed based on the number of accessions per region. It is expected that the findings of the present study can provide basic data for future studies on ginseng genetic diversity and for breeding ginseng cultivars.

show abstract

“…미국, 캐나다 등 인삼산업 경쟁국과의 자유무역협정이 발효되 면 더욱 더 그 피해가 심각해질 것으로 전문가들은 예상하고 있다 (Bang et al, 2011a). 또한 홍콩 등 해외시장에서 한국 산 고려인삼에 대한 소비자 인식이 좋아 국내 인삼 제품의 모 조품까지 유통되고 있는 실정이다.…”

unclassified

Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species

Jo¹,

Bang²,

Kim³

et al. 2013

Korean Journal of Medicinal Crop Science

View full text Add to dashboard Cite

: This study describes the identification of Panax species using mitochondrial consensus primers. Initially, a total of thirty primers were tested in ten Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, three primers (cox1, nad1/2-3 and nad2/1-2) generated co-dominant polymorphic banding patterns discriminating Korean ginseng cultivars from P. quinquefolius and P. notoginseng. However, these primers could not generated polymorphisms among the Korean ginseng cultivars, and simply represented species-specific polymorphisms for P. quinquefolius and P. notoginseng. Primers PQ91 and PN418 were designed from the consensus sequence of nad1/2-3 region. Two banding patterns (A or B) were detected in PQ91. Korean ginseng cultivars and P. notoginseng shared the same banding pattern (A type) and P. quinquefolius was identified another banding pattern (B type). In the case of PN418, two banding patterns (A or B) were detected in the Korean ginseng cultivars and two foreign Panax species. Korean ginseng cultivars and P. quinquefolius shared the same banding pattern (A type) and P. notoginseng was identified another banding pattern (B type). The combination banding patterns of three Panax species, Korean ginseng cultivars (Panax ginseng C. A. Mey.), P. quinquefolius and P. notoginseng, was identified as 'AA', 'BA' and 'AB', respectively. Consequently, PQ91 and PN418 primer sets can be used to distinguish among Panax species.

show abstract

Development of SSR Markers for Identification of Korean Ginseng (Panax ginseng C. A. Meyer) Cultivars

Cited by 17 publications

References 13 publications

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

Genetic diversity and population structure of Chinese ginseng accessions using SSR markers

Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species

Contact Info

Product

Resources

About