Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species

Jo, Ick-Hyun; Bang, Kyong-Hwan; Kim, Young Chang; Kim, Jang Uk; Shin, Mi Ran; Moon, Ji Young; Noh, Bong Soo; Hyun, Dong Yun; Kim, Dong Hwi; Woo, Seon Rang; Kim, Hong Sig

doi:10.7783/kjmcs.2013.21.2.91

Cited by 15 publications

(5 citation statements)

References 17 publications

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“…Việc xác định chính xác các loài/thứ của Sâm là rất quan trọng đối với việc xác định giống cây, lựa chọn cha mẹ để nhân giống, sử dụng và bảo tồn nguồn gen của chúng. Các phương pháp truyền thống xác định các loài trong chi Panax chủ yếu dựa trên các quan sát kiểu hình trong khi các đặc điểm hình thái thường bị ảnh hưởng bởi các yếu tố môi trường (Jo et al, 2013). Đặc biệt, trong giai đoạn chưa ra hoa, quả, đặc điểm hình thái của nhiều loài Sâm rất tương đồng, khiến việc phân biệt chúng rất khó khăn.…”

Section: Mở đầUunclassified

Using ITS-rDNA and matK gene nucleotide sequences for identification ginseng species in Panax in Phu Xai Lai Leng, Ky Son, Nghe An

Duy¹,

Thanh²,

Loc³

et al. 2020

Vietnam J. Biotechnol.

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DNA barcoding is a useful tool for species identification using standardized genomic DNA fragments. We used DNA barcodes (ITS-rDNA and matK gene) to explore Panax (32 samples collected from Phu Xai Lai Leng mountain and 19 samples collected from medicinal nursery of TH), and to investigate the phylogenetic taxonomy of Panax. In this study, the PCR success rate for ITS-rDNA and matK region was 100%. The success rate of bidirectional sequencing of PCR product was 100% of ITS-rDNA and matK region with length of 616 bp, 1433 bp, respectively. All 32 samples (Panax TB) of Phu Xai Lai Leng have a close relationship with P. stipuleanatus (MLBS = 99%, BPP = 100%). All 19 samples (Panax TH) of medicinal nursery have a close relationship with P. notoginseng (MLBS = 100%, BPP = 100%). Interspecific genetic distances within and among Panax species was varied from 0.2% to 7.9%, average (4%) (ITS-rDNA gene) and 0.1 to 2.9%, average (1.2%) (matK gene). The genetic relationship of species/gender belonging to the Panax genus showed that they have the same evolutionary origin and discovered that new distributed of P. stipuleanatus in Phu Xai Lai Leng mountain in Vietnam.

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Section: Mở đầUunclassified

Using ITS-rDNA and matK gene nucleotide sequences for identification ginseng species in Panax in Phu Xai Lai Leng, Ky Son, Nghe An

Duy¹,

Thanh²,

Loc³

et al. 2020

Vietnam J. Biotechnol.

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“…Many studies identify plants species and varieties using morphological, cytological, anatomical, physiological, and ecological methods (Woo et al 2004;Yu et al 2009;Jee et al 2014;Bai et al 2015;Kim et al 2015). Using such morphological characteristics to distinguish among varieties can lead to confusion because expression may be the result of a combination of genetics and environment (Jo et al 2013). Furthermore, molecular techniques have added another level of classification through study of DNA (Lim and Choi 1990;Lim et al 1993;Hon et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

Um¹,

Mitsugi²,

Kim³

et al. 2016

Plant Breed. Biotech

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2016 (February) ABSTRACT Panax ginseng has been one of the most important herbal medicines used in Eastern Asia. Recently, various molecular markers have been developed to authenticate Panax species, but these markers cannot differentiate the exact varieties or variants of Korean ginseng cultivars. In this study, six cultivars of Korean ginseng (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng were differentiated by simple sequence repeat (SSR) marker development. Specific primer sets were designed for the 54 candidate sequences containing SSRs that were predicted. Finally, eight polymorphic SSR loci were developed. DNA fragment analysis was performed using fluorescence-labelled primers for the amplicons. Reproducibility tests were carried out using multiple samples of Korean ginseng cultivars and Panax species. Eight primer sets (PgSSR07, PgSSR08, PgSSR09, PgSSR17, PgSSR37, PgSSR40, PgSSR51, and PgSSR53) showing polymorphism were used for phylogenetic relationship analysis. Consequently, six Korean ginseng cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng could be identified using the combination of SSR markers discovered.

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“…RAPD and PCR-RFLP markers show insufficient numbers of suitable loci within the genome and have poor sensitivity, reliability, discriminatory ability, and reproducibility, posing difficulties in terms of discrimination of strains [35] . In contrast, an STS marker with the simplicity of its analytical method allows for easy confirmation of results with high reproducibility and, therefore, can be applied to the analysis of massive genetic resources and to cultivar discrimination [36] , [37] .…”

Section: Molecular Markers For Discrimination In Panax mentioning

confidence: 99%

Applications of molecular markers in the discrimination of Panax species and Korean ginseng cultivars ( Panax ginseng )

Kim

et al. 2017

Journal of Ginseng Research

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The development of molecular markers is one of the most useful methods for molecular breeding and marker-based molecular associated selections. Even though there is less information on the reference genome, molecular markers are indispensable tools for determination of genetic variation and identification of species with high levels of accuracy and reproducibility. The demand for molecular approaches for marker-based breeding and genetic discriminations in Panax species has greatly increased in recent times and has been successfully applied for various purposes. However, owing to the existence of diverse molecular techniques and differences in their principles and applications, there should be careful consideration while selecting appropriate marker types. In this review, we outline the recent status of different molecular marker applications in ginseng research and industrial fields. In addition, we discuss the basic principles, requirements, and advantages and disadvantages of the most widely used molecular markers, including restriction fragment length polymorphism, random amplified polymorphic DNA, sequence tag sites, simple sequence repeats, and single nucleotide polymorphisms.

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Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species

Cited by 15 publications

References 17 publications

Using ITS-rDNA and matK gene nucleotide sequences for identification ginseng species in Panax in Phu Xai Lai Leng, Ky Son, Nghe An

Using ITS-rDNA and matK gene nucleotide sequences for identification ginseng species in Panax in Phu Xai Lai Leng, Ky Son, Nghe An

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

Applications of molecular markers in the discrimination of Panax species and Korean ginseng cultivars ( Panax ginseng )

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